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Volume 78, Issue 2


Non-invasive genetics can help find rare species: a case study with Rhinolophus mehelyi and R. euryale (Rhinolophidae: Chiroptera) in Western Europe

Sébastien J. Puechmaille
  • Corresponding author
  • UCD School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland
  • Sensory Ecology Group, Max Planck Institute for Ornithology, 82319 Seewiesen, Germany; and Groupe Chiroptères de Midi-Pyrénées (CREN-GCMP), Toulouse, France
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Emma C. Teeling
  • UCD School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2013-09-04 | DOI: https://doi.org/10.1515/mammalia-2013-0040


The reliability of species identification is of primary importance as much of biodiversity studies, ecology, legislation, and conservation are based on this taxonomic level. Species identification problems can obscure the conservation status, especially for rare and endangered species, which are of special concern for conservation. This problem is especially significant for some taxonomic groups such as chiropterans, as many monitoring programs are run during the hibernation season when animals should not be disturbed, hence not handled. In the present study, we used Rhinolophus mehelyi as a case study to develop and propose a new monitoring strategy via the use of non-invasive genetics to reliably identify individuals to species.

Keywords: Chiroptera; monitoring; non-invasive genetics; species identification; threatened species


  • Berthier, P., L. Excoffier and M. Ruedi. 2006. Recurrent replacement of mtDNA and cryptic hybridization between two sibling bat species Myotis myotis and Myotis blythii. Proc. R. Soc. Lond. B 273: 3101–3109.Google Scholar

  • Boston, E.S.M., D.J. Buckley, M. Bekaert, Y. Gager, M.G. Lundy, D.D. Scott, P.A. Prodohl, W.I. Montgomery, F. Marnell and E.C. Teeling. 2010. The status of the cryptic bat species, Myotis mystacinus and Myotis brandtii in Ireland. Acta Chiropt. 12: 457–461.CrossrefWeb of ScienceGoogle Scholar

  • Boston, E.S.M., S.J. Puechmaille, D.D. Scott, D.J. Buckley, M.G. Lundy, W.I. Montgomery, P.A. Prodohl and E.C. Teeling. 2012. Empirical assessment of non-invasive population genetics in bats: comparison of DNA quality from faecal and tissue samples. Acta Chiropt. 14: 45–52.CrossrefGoogle Scholar

  • Broquet, T., N. Ménard and E. Petit. 2007. Noninvasive population genetics: a review of sample source, diet, fragment length and microsatellite motif effects on amplification success and genotyping error rates. Conserv. Genet. 8: 249–260.Google Scholar

  • Dool, S., S.J. Puechmaille, C. Dietz, J. Juste, C. Ibáñez, P. Hulva, S.G. Roué, E.J. Petit, G. Jones, D. Russo, R. Toffoli, A. Viglino, A. Martinoli, S.J. Rossiter and E.C. Teeling. 2013. Phylogeography and postglacial recolonisation of Europe by Rhinolophus hipposideros: evidence from multiple genetic markers. Mol. Ecol. 22: 4055–4070.CrossrefGoogle Scholar

  • Dragu, A. and I. Borissov. 2011. Low genetic variability of Rhinolophus mehelyi (Mehely’s horseshoe bat) in Romania. Acta Theriol. 56: 383–387.Web of ScienceCrossrefGoogle Scholar

  • Eggert, L.S., J.E. Maldonado and R.C. Fleischer. 2005. Nucleic acid isolation from ecological samples – animal scat and other associated materials. Methods Enzymol. 395: 73–87.Web of ScienceGoogle Scholar

  • Flaquer, C., X. Puig, E. Fàbregas, D. Guixé, I. Torre, R.G. Ràfolos, F. Páramo, J. Camprodon, J.M. Cumplido, R. Ruiz-Jarillo, A.L. Baucells, L. Freixas and A. Arrizabalaga. 2010. Revisión y aportación de datos sobre quirópteros de Catalunya: propuesta de lista roja. Galemys 22: 29–61.Google Scholar

  • Gallocher, P. 1958. Contribution à l’étude des chiroptères en Basse-Provence, 1954–1958. Bull. Mens. Gr. Spéléol. Sect. Prov. C.A.F. 31: 1–18.Google Scholar

  • Goodman, S.M., S.J. Puechmaille, N. Friedli-Weyeneth, J. Gerlach, M. Ruedi, M.C. Schoeman, W.T. Stanley and E.C. Teeling. 2012. Phylogeny of the Emballonurini (Emballonuridae) with descriptions of a new genus and species from Madagascar. J. Mammal. 93: 1440–1455.Web of ScienceCrossrefGoogle Scholar

  • Gory, G. and R. Jeantet. 1999. Eléments de biométrie de huit espèces de chiroptères capturées en région méditerranéenne. Bul. Soc. Et. Sci. Nat. Nîmes Gard 62: 75–79.Google Scholar

  • Hugues, A. 1914. Les chiroptères du département du Gard. Zoologie systématique, Moeurs des Animaux. In: 9ème Congrés International de Zoologie, Monaco. Sect. 3, pp. 477–483.Google Scholar

  • Hugues, A. 1936. Passage et baguage des chauves-souris. La Nature 64: 33–34.Google Scholar

  • Jeantet, R. and G. Gory. 1997. Le baguage de chiroptères en région méditerranéenne de 1958 à 1970. Bul. Soc. Et. Sci. Nat. Nîmes Gard 61: 85–88.Google Scholar

  • Kahmann, H. and P. Goerner. 1956. Les Chiroptères de Corse. Mammalia 20: 333–389.Google Scholar

  • Kohn, M.H. and R.K. Wayne. 1997. Facts from feces revisited. Trends Ecol. Evol. 12: 223–227.Google Scholar

  • König, C. and I. König. 1961. Zur Ökologie und systematik südfranzösischer fledermäuse. Bonn. Zool. Beitr. 12: 189–230.Google Scholar

  • Luikart, G., N. Ryman, D.A. Tallmon, M.K. Schwartz and F.W. Allendorf. 2010. Estimation of census and effective population sizes: the increasing usefulness of DNA-based approaches. Conserv. Genet. 11: 355–373.Web of ScienceCrossrefGoogle Scholar

  • Masson, D. and J.-P. Besson. 1988. Rhinolophus mehelyi Matschie, 1901 (Chiroptera: Rhinolophidae) dans le sud-ouest de la France. Mammalia 52: 275–278.Google Scholar

  • Miller, G.S. 1912. Catalogue of the mammals of Western Europe (Europe exclusive of Russia). British Museum (Natural History), London. pp. 1019.Google Scholar

  • Mottaz, C. 1908. Quelques intéressantes captures de petits Mammifères. Bull. Soc. Zool. Genève 1: 178–179.Google Scholar

  • Posada, D. and K.A. Crandall. 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817–818.CrossrefPubMedGoogle Scholar

  • Puechmaille, S.J. and E.J. Petit. 2007. Empirical evaluation of non-invasive capture-mark-recapture estimates of population size based on a single sampling session. J. Appl. Ecol. 44: 843–852.Web of ScienceCrossrefGoogle Scholar

  • Puechmaille, S.J., G. Mathy and E. Petit. 2005. Characterization of 14 polymorphic microsatellite loci for the lesser horseshoe bat, Rhinolophus hipposideros (Rhinolophidae, Chiroptera). Mol. Ecol. Notes 5: 941–944.Google Scholar

  • Puechmaille, S.J., G. Mathy and E.J. Petit. 2007. Good DNA from bat droppings. Acta Chiropt. 9: 269–276.CrossrefGoogle Scholar

  • Puechmaille, S.J., M. Ar Gouilh, P. Piyapan, M. Yokubol, Khin Mie Mie, P.J.J. Bates, C. Satasook, Tin Nwe, Si Si Hla Bu, I.J. Mackie, E.J. Petit and E.C. Teeling. 2011. The evolution of sensory divergence in the context of limited gene flow in the bumblebee bat. Nat. Commun. 2: 573.CrossrefGoogle Scholar

  • Puechmaille, S.J., W.M. Hizem, B. Allegrini and A. Abiadh. 2012. Bat fauna of Tunisia: review of records and new records, morphometrics and echolocation data. Vespertilio 16: 211–239.Google Scholar

  • Rode, P. 1939. Les Mammifères de la collection Mottaz (suite) II. Les Chiroptères. Bull. Mus. Hist. Nat., Paris 11: 274–278.Google Scholar

  • Rodrigues, A.S.L., J.D. Pilgrim, J.F. Lamoureux, M. Hoffmann and T.M. Brooks. 2006. The value of the IUCN Red List for conservation. Trends Ecol. Evol. 21: 71–76.Google Scholar

  • Rombaut, D. and A. Haquart. 2002. Les Chiroptères de la directive Habitat: le Rhinolophe de Mehely Rhinolophus mehelyi Matschie, 1901. Arvicola 14: 18–20.Google Scholar

  • Ruedi, M., N. Friedli-Weyeneth, E.C. Teeling, S.J. Puechmaille and S.M. Goodman. 2012. Biogeography of Old World emballonurine bats (Chiroptera: Emballonuridae) inferred with mitochondrial and nuclear DNA. Mol. Phylogenet. Evol. 64: 204–211.CrossrefPubMedWeb of ScienceGoogle Scholar

  • Ruffray, V. 2011. Les gîtes importants pour la conservation des chiroptères de l’annexe II en Languedoc-Roussillon. Vespère 2: 124–180.Google Scholar

  • Salsamendi, E., I. Garin, D. Almenar, U. Goiti, M. Napal and J.R. Aihartza. 2008. Diet and prey selection in Mehelyi’s horseshoe bat Rhinolophus mehelyi (Chiroptera, Rhinolophidae) in the south-western Iberian Peninsula. Acta Chiropt. 10: 279–286.CrossrefWeb of ScienceGoogle Scholar

  • Strinati, P. and V. Aellen. 1958. Confirmation de la présence de Rhinolophus mehelyi Matschie dans le sud de la France. Mammalia 22: 527–536.Google Scholar

  • Swofford, D.L. 2003. PAUP*. Phylogenetic Analysis Using Parsimony (* and Other Methods). Sinauer Associates, Sunderland, MA.Google Scholar

  • Taberlet, P., L.P. Waits and G. Luikart. 1999. Noninvasive genetic sampling: look before you leap. Trends Ecol. Evol. 14: 323–327.Google Scholar

  • Thong, V.D., S.J. Puechmaille, A. Denzinger, P.J.J. Bates, C. Dietz, G. Csorba, P. Soisook, E.C. Teeling, S. Matsumura, N. Furey and H.U. Schnitzler. 2012. Systematics of the Hipposideros turpis complex and a description of a new subspecies from Vietnam. Mammal Rev. 42: 166–192.Web of ScienceCrossrefGoogle Scholar

  • Vege, S. and G.F. McCracken. 2001. Microsatellite genotypes of big brown bats (Eptesicus fuscus: Vespertilionidae, Chiroptera) obtained from their faeces. Acta Chiropt. 3: 237–244.Google Scholar

  • Višňovská, Z., J. Zelinka and K. Strug. 2006. Spatial distribution of hibernating bats (Chiroptera) in relation to climatic conditions in the Demänovská ice cave (Slovakia). In: (J. Zelinka, ed.) Proceedings of the 2nd International Workshop on ice caves, Demänovská Dolina. Slovak Caves Administration, Liptovský Mikuláš, Slovakia. pp. 87–97.Google Scholar

  • Webb, P.I., J.R. Speakman and P.A. Racey. 1996. How hot is a hibernaculum? A review of the temperatures at which bats hibernate. Can. J. Zool. 74: 761–765.CrossrefGoogle Scholar

  • Zinck, J., D.A. Duffield and P.C. Ormsbee. 2004. Primers for identification and polymorphism assessment of Vespertilionid bats in the Pacific Northwest. Mol. Ecol. Notes 4: 239–242.CrossrefGoogle Scholar

About the article

Corresponding author: Sébastien J. Puechmaille, UCD School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland, e-mail: ; and Sensory Ecology Group, Max Planck Institute for Ornithology, 82319 Seewiesen, Germany; and Groupe Chiroptères de Midi-Pyrénées (CREN-GCMP), Toulouse, France

aPresent address: Zoological Institute and Museum, Greifswald University, 17489 Greifswald, Germany.

Received: 2013-03-08

Accepted: 2013-08-06

Published Online: 2013-09-04

Published in Print: 2014-05-01

Citation Information: Mammalia, Volume 78, Issue 2, Pages 251–255, ISSN (Online) 1864-1547, ISSN (Print) 0025-1461, DOI: https://doi.org/10.1515/mammalia-2013-0040.

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