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Open Medicine

formerly Central European Journal of Medicine

Editor-in-Chief: Darzynkiewicz, Zbigniew


IMPACT FACTOR 2018: 1.221

CiteScore 2018: 1.01

SCImago Journal Rank (SJR) 2018: 0.329
Source Normalized Impact per Paper (SNIP) 2018: 0.479

ICV 2018: 156.09

Open Access
Online
ISSN
2391-5463
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Volume 8, Issue 3

Issues

Volume 10 (2015)

Vimentin cleavage in end-stage renal disease is not related to apoptosis

Felix Liebscher / Tobias Arnold / Ying Liang / Thomas Reiter / Georg Böhmig / Rudolf Oehler
Published Online: 2013-04-17 | DOI: https://doi.org/10.2478/s11536-012-0131-8

Abstract

Anti-vimentin auto-antibodies contribute to chronic allograft nephropathy. They exist in sera of end-stage renal disease patients on hemodialysis (ESRD) already before renal transplantation. We found recently that a 49 kDa vimentin fragment is increased in lymphocytes of ESRD patients which is presented on the cell surface. In vitro studies showed that such a fragment is formed during apoptosis by active caspase-3. We hypothesized that vimentin degradation in leukocytes of ESRD patients correlates to caspase-3 activation in vivo. Lymphocytes and monocytes were isolated from ESRD patients and from healthy volunteers and analyzed for vimentin expression and caspase-3 activation. In addition, apoptosis was induced in vitro and quantified by flow cytometry. ESRD monocytes have shown only the full length 60 kDa vimentin isoform. ESRD lymphocytes, however, showed in addition a strongly increased expression of the 49 kDa vimentin in all samples. Caspase-3 activation was found in 60% of ESRD lymphocytes and 66% of ESRD monocytes but not in healthy volunteers. UV-mediated induction of apoptosis was not associated with vimentin degradation. These experiments could confirm increased vimentin degradation in ESRD lymphocytes. However, we could not validate any correlation to apoptosis.

Keywords: Vimentin; Apoptosis; Caspase-3; End-stage renal disease

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About the article

Published Online: 2013-04-17

Published in Print: 2013-06-01


Citation Information: Open Medicine, Volume 8, Issue 3, Pages 297–301, ISSN (Online) 2391-5463, DOI: https://doi.org/10.2478/s11536-012-0131-8.

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© 2013 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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