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Open Medicine

formerly Central European Journal of Medicine

Editor-in-Chief: Darzynkiewicz, Zbigniew


IMPACT FACTOR 2018: 1.221

CiteScore 2018: 1.01

SCImago Journal Rank (SJR) 2018: 0.329
Source Normalized Impact per Paper (SNIP) 2018: 0.479

ICV 2017: 152.94

Open Access
Online
ISSN
2391-5463
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Volume 10, Issue 1

Issues

Volume 10 (2015)

Quick and effective method of bone marrow mesenchymal stem cell extraction

Zivile Gudleviciene / Gabrielis Kundrotas
  • National Cancer Institute, Biobank Santariskiu 1, 08660 Vilnius, Lithuania Vilnius University, Faculty of Natural Sciences M.K. Ciurlionio 21/27, 03101 Vilnius
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Regina Liudkeviciene / Jelena Rascon
  • Children‘s Hospital, Affiliate of Vilnius Unversity Hospital Santariskiu Klinikos, Children Heamatology and Transplantation Center Santariskiu 7, 08406, Vilnius, Lithuania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Marcin Jurga
  • Cryo-Save Labs NV, Attn. The Cell Factory (R&D department) Galileilaan 19, B-2845 Niel, Belgium
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-10-08 | DOI: https://doi.org/10.1515/med-2015-0008

Abstract

Background. Mesenchymal stem cells (MSCs) are currently exploited in numerous clinical trials to investigate their potential in immune regulation, hematopoesis or tissue regeneration. The most common source of MSCs for clinical use is human bone marrow. To generate sufficient numbers of cells relevant to clinical use in most cases the high volumes (20-50 ml) of bone marrow aspirates are taken. Methods. In this pilot study, 8 healthy bone marrow donors were included. Two different MSC extraction methods were evaluated: MSCs extraction from 60 ml of bone marrow using density gradient and MSCs extraction from 6 ml using red blood cell (RBC) lysis. Results. Our results showed that after RBC lysis the efficient amount of human MSCs can be isolated from 10 times less bone marrow volume (6 ml). Moreover, using small volume of bone marrow the adequate therapeutical dose of MSCs could be achieved during similar period of time (3-4 weeks). In conclusion, we have shown that MSCs isolation using RBC lysis is an effective and more advantageous method in comparison to standard MSCs isolation using density-gradient. Using RBC lysis from small volume of bone marrow the same amount of MSCs were obtained as usually using large volume and density-gradient.

Keywords : Bone marrow; Cell therapy; Ficoll gradient; Mesenchymal stem cells; Red blood cell lysis

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About the article

Received: 2014-03-21

Accepted: 2014-06-29

Published Online: 2014-10-08

Published in Print: 2015-01-01


Citation Information: Open Medicine, Volume 10, Issue 1, ISSN (Online) 2391-5463, DOI: https://doi.org/10.1515/med-2015-0008.

Export Citation

© 2015 Z. Gudleviciene et al,. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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