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Non-coding RNAs in Endocrinology

Ed. by Hardikar, Anandwardhan

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Emerging Science

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Combined Immuno-FISH confirms islet-specific miRNAs

Wilson Wong / Malati R. Umrani / Mugdha V. Joglekar
  • Corresponding author
  • Diabetes and Islet Biology Group, NHMRC Clinical Trials Centre, The University of Sydney, Level 6, Medical Foundation Building, 92-94 Parramatta Road, Camperdown, NSW 2050, Australia
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Published Online: 2017-11-11 | DOI: https://doi.org/10.1515/micrnado-2017-0002


Combined immuno-FISH offers an important tool for understanding the localization of mature microRNAs with reference to other proteins within the same cell. This procedure has been previously demonstrated by our team and the method has been described earlier [1, 2].

Keywords: microRNA-375; human islets; diabetes


  • [1] Gershengorn, M.C. , Hardikar, A.A. , Wei, C. , Geras-Raaka, E. , Marcus-Samuels, B. , and Raaka, B.M., Epithelial-tomesenchymal transition generates proliferative human islet precursor cells. Science, 2004. 306(5705): p. 2261-4. Google Scholar

  • [2] Joglekar, M.V. , Joglekar, V.M. , and Hardikar, A.A., Expression of islet-specific microRNAs during human pancreatic development. Gene Expr Patterns, 2009. 9(2): p. 109-13.CrossrefGoogle Scholar

About the article

Received: 2016-05-18

Accepted: 2016-05-18

Published Online: 2017-11-11

Published in Print: 2017-11-11

Citation Information: Non-coding RNAs in Endocrinology, Volume 2, Issue 1, Pages 4–5, ISSN (Online) 2300-4258, DOI: https://doi.org/10.1515/micrnado-2017-0002.

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© 2017. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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