Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Molecular Imprinting

Published in Association with Society for Molecular Imprinting

1 Issue per year


Emerging Science

Open Access
Online
ISSN
2084-8803
See all formats and pricing
More options …

Molecularly Imprinted Polymer Receptors for Nicotine Recognition in Biological systems

Reddithota J. Krupadam / Avinash Venkatesh
  • Department of Chemical Engineering, National Institute of Technology Karnataka Suratkal, Mangalore, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sergy A. Piletsky
Published Online: 2013-01-16 | DOI: https://doi.org/10.2478/molim-2012-0004

Abstract

Molecular imprinting of nicotine and related carcinogenic chemicals in itaconic acid – ethylene glycol dimethacrylate copolymer is described. Molecularly imprinted polymers (MIPs) are made to contain binding sites capable of recognizing nicotine; thus the fingerprint of the nicotine created in the polymer allows it to serve as an ideal molecular recognition element. We demonstrate that the imprinted polymers show high selective binding affinity in biological buffers. This is a previously un-described initiative for molecular imprinting, since the binding occurs under conditions relevant to biological systems. Due to effect of molecular imprinting nanocavities with size 24±5 nm were formed and these nicotine receptor sites were distributed homogeneously in the nicotine imprinted polymer. The nicotine receptors showed highly selective to nicotine with Kd values as low as 10-5M, and the levels of selectivity similar to those of natural molecules - acetylcholine esterase (AChE). The recognition properties of the polymer receptor were analyzed using ultra-violet spectroscopy, computer simulations and adsorption assay. Importantly, the receptors were effective in wide pH range (6.8-8.2) while the natural nicotine receptors showed high binding only at pH 7.6. The high specificity and stability of artificial receptors rendered them promising alternatives to enzymes, antibodies, and other natural receptors useful in biomedical assays, sensors and drug development.

This article offers supplementary material which is provided at the end of the article.

Keywords: Molecular imprinting; Molecular modeling; Nicotine receptors; Polymer receptors; Selective binding

  • G. MacBeath, Nat. Genetics 32, 526 (2002) Google Scholar

  • a) A. Giannis, T. Kolter, Angew. Chem. Int. Ed. 32, 1244 (1993); b) V.J. Hruby, P.M. Balse, Current Med. Chem. 7, 945 (2000) Google Scholar

  • a) L.I. Andersson, R. Müller, G. Vlatakis, K. Mosbach, Proc. Natl. Acad. Sci. USA, 92, 4788 (1995); b) S.C. Zimmerman, I. Zharov, M.S. Wendland, N.A. Rakow, K.S. Suslick, J. Am. Chem. Soc. 125, 13504 (2003) Google Scholar

  • a) G. Vlatakis, L.I. Andersson, R. Mueller, K. Mosbach, Nature 361, 645 (1993); b) B.R. Hart, K.J. Shea, J. Am. Chem. Soc. 123, 2072 (2001); c) B. Sellergren, Angew. Chem. Int. Ed. 39, 1031 (2000) Google Scholar

  • C.J. Tan, Y.W. Tong, Langmuir 23, 2722 (2007) Google Scholar

  • J. Matsui, T.A. Takeuchi, Anal. Commun. 34, 199 (1997) Google Scholar

  • M.W. Mullett, E.P.C. Lai, B. Sellergren, Anal. Commun. 36, 217 (1999) Google Scholar

  • J. Svenson, J.G. Karlsson, I.A. Nicholls, J. Chromatogr. A 1024, 39 (2004) Google Scholar

  • M. Sibrian-Vazquez, D.A. Spivak, J. Am. Chem. Soc. 126, 7827 (2004) Google Scholar

  • a) R.J. Umpleby II, S.C. Baxter, Y. Chen, R.N. Shah, K.D. Shimizu, Anal. Chem. 73, 4584 (2001); b) G.T. Rushton, C.L. Karns, K.D. Shimizu, Anal. Chim. Acta 528, 107 (2005) Google Scholar

  • A. Zander, P. Findlay, T. Renner, B. Sellergren, Anal. Chem. 70, 3304 (1998)PubMedGoogle Scholar

About the article


Received: 2012-07-25

Accepted: 2012-12-16

Published Online: 2013-01-16


Citation Information: Molecular Imprinting, ISSN (Online) 2084-8803, DOI: https://doi.org/10.2478/molim-2012-0004.

Export Citation

©2012 Versita Sp. z o.o.. This content is open access.

Supplementary Article Materials

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Erkut Yılmaz, Bora Garipcan, Hirak Patra, and Lokman Uzun
Sensors, 2017, Volume 17, Number 4, Page 691
[2]
Haruka NOGAMI, Yuma NAKAHORI, Takashi MURASHIMA, and Jun MATSUI
CHROMATOGRAPHY, 2017, Volume 38, Number 1, Page 15
[3]
Wei Chen, Yue Ma, Jianmin Pan, Zihui Meng, Guoqing Pan, and Börje Sellergren
Polymers, 2015, Volume 7, Number 9, Page 1689

Comments (0)

Please log in or register to comment.
Log in