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Molecular Imprinting

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MIP-based immunoassays: State of the Art, limitations and Perspectives

Claudio Baggiani
  • Cardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University, King Edward VII Avenue, Cardiff, CF10 3NB, United Kingdom
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/ Laura Anfossi
  • Cardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University, King Edward VII Avenue, Cardiff, CF10 3NB, United Kingdom
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/ Cristina Giovannoli
  • Cardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University, King Edward VII Avenue, Cardiff, CF10 3NB, United Kingdom
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Published Online: 2013-08-13 | DOI: https://doi.org/10.2478/molim-2013-0002

Abstract

Immunoassay is one of the most popular analytical methods with widespread applications. However, it presents several drawbacks because of the proteic nature of the antibodies. Molecular imprinting technology has shown a growing ability to prepare artificial molecular recognition systems, with binding properties very similar to those of natural antibodies. This review deals with the application of molecular imprinting technology to immunoassay, with an attention for the state of the art, the current limitations and the possible solutions to these issues.

Keywords: Molecularly imprinted polymers; Plastibodies; Molecularly imprinted sorbent assay; Immunoassay; Radioimmunoassay; Enzyme immunoassay; Fluoroimmunoassay

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About the article


Received: 2013-04-09

Accepted: 2013-06-12

Published Online: 2013-08-13


Citation Information: Molecular Imprinting, Volume 1, Pages 41–54, ISSN (Online) 2084-8803, DOI: https://doi.org/10.2478/molim-2013-0002.

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©2014 Claudio Baggiani et al.. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial No-Derivatives License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. BY-NC-ND 3.0

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