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Molecular Imprinting

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Pressurized hot water extraction coupled to molecularly imprinted polymers for simultaneous extraction and clean-up of pesticides residues in edible and medicinal plants of the Okavango Delta, Botswana

Janes Mokgadi / Shima Batlokwa
  • Department of Earth & Environmental Science (Chemistry Unit), Botswana International University of Science & Technology, P.O. Box 041, Bontleng, Gaborone, Botswana
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Keta Mosepele / Veronica Obuseng / Nelson Torto
Published Online: 2013-10-08 | DOI: https://doi.org/10.2478/molim-2013-0003

Abstract

In this study, an in-cell extraction and clean-up approach, employing pressurized hot water extraction (PHWE) coupled to a molecularly imprinted polymer (MIP) is proposed. The selectivity of PHWE was improved through the use of a chlorophyll MIP (PHWE-MIP) for the determination of organochlorine pesticides residue levels in various edible and medicinal plants of the Okavango Delta, Botswana. The PHWE-MIP method achieved simultaneous extraction and clean-up. PHWE employed an optimal temperature of 260 °C, pressure of 90 bar and flow rate of 1 mL min-1 in 10 min for the extraction of the pesticides from plants while the MIP selectively overcame the interfering chlorophyll prior to analysis with gas chromatograph coupled to electron capture detector or mass spectrometer (GC-ECD/MS). The results obtained were compared to the QuEChERS Official Method 2007:01 for pesticides residue analysis. The proposed method seems to be nearly fully automated, environmental friendly, selective, simple and quick. Moreover, the recoveries of planar pesticides were improved (93-95%) with relative standard deviations (%RSD) of less than 10%.

Keywords: Subcritical water extraction; Molecular imprinted polymers; In-cell clean up; Sample preparation; Environmentally friendly

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About the article


Received: 2013-03-15

Accepted: 2013-08-28

Published Online: 2013-10-08


Citation Information: Molecular Imprinting, Volume 1, Pages 55–64, ISSN (Online) 2084-8803, DOI: https://doi.org/10.2478/molim-2013-0003.

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©2014 Janes Mokgadi et al.. This article is distributed under the terms of the Creative Commons Attribution Non-Commercial No-Derivatives License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. BY-NC-ND 3.0

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