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Molecular Imprinting

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ATP-binding peptide-hydrogel composite synthesized by molecular imprinting on beads

Ayana Takata
  • Department of Nanobiochemistry, FIRST, Konan University, 7-1-20 Minatojima-minami-machi, Chuo-ku, Kobe 650-0047, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kenji Usui
  • Department of Nanobiochemistry, FIRST, Konan University, 7-1-20 Minatojima-minami-machi, Chuo-ku, Kobe 650-0047, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jun Matsui
  • Department of Nanobiochemistry, FIRST, Konan University, 7-1-20 Minatojima-minami-machi, Chuo-ku, Kobe 650-0047, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-12-31 | DOI: https://doi.org/10.1515/molim-2015-0008

Abstract

Molecular imprinting has been recognized as a useful technique to produce synthetic mimics of functional proteins, such as antibodies and enzymes. However, only a few studies have examined peptides as starting materials for synthesizing molecularly imprinted polymers in spite of the expectation that peptides would be suitable materials for realizing water-compatibility and proteinlike functions. In this study, molecular imprinting was performed using a vinyl-end-capped on-beads-peptide as functional monomer to produce an on-beads-peptide hydrogel composite selective for ATP; the on-beadspeptide peptide, of which sequence was designed to possess both an adenine-recognition site and phosphate recognition site, was co-polymerized with NIPAM and BIS in the presence of ATP as a template species. The resultant ATP-imprinted composite showed 14-times higher affinity and an enhanced selectivity towards ATP, suggesting that the peptide conformation, i.e. a mutual orientation of the two binding sites, was pre-organized and immobilized in a manner where the ATP binding is more favored.

Keywords: peptide; hydrogel; ATP

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About the article

Received: 2015-08-03

Accepted: 2015-12-28

Published Online: 2015-12-31


Citation Information: Molecular Imprinting, Volume 3, Issue 1, Pages 65–70, ISSN (Online) 2084-8803, DOI: https://doi.org/10.1515/molim-2015-0008.

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© 2016 Ayana Takata et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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