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Molecular Imprinting

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Application of a Custom-Synthesized Molecularly Imprinted Polymer for the Selective Isolation of Total Glucose and Fructose from 100% Fruit Juice Samples Prior to Instrumental Analysis

Hawa W. Mukami
  • Corresponding author
  • Botswana International University of Science and Technology, P.O Box private bag 16 Palapye, Botswana
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Bareki S. Batlokwa
  • Botswana International University of Science and Technology, P.O Box private bag 16 Palapye, Botswana
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2018-09-19 | DOI: https://doi.org/10.1515/molim-2018-0001


This article presents a novel sample preparation strategy that employed a custom-synthesized glucose - fructose (G-F) specific molecularly imprinted polymer (MIP) powder as an adsorbent for the simultaneous and selective extraction, isolation and pre-concentration of total glucose and fructose from the complex and `dirty` sample matrix of ‘100%’ fruit juices purchased from retail shops in Palapye, Botswana. The prepared G-F MIP powder demonstrated high selectivity, effective extraction and isolation for glucose and fructose from real samples of `100%` fruit juice samples as evidenced by the calculated high extraction efficiencies (EEs) of over 90%, with low percentage relative standard deviations (%RSD) of below 7% for n=6, for both glucose and fructose when compared to the low EEs of below 25% by the non-imprinted polymer (NIP), regarded as the control. Furthermore, the G-F MIP showed lower selectivity towards the analogous molecules; maltose and lactose as supported by the low EEs of below 31%. With the high affinity for glucose and fructose, the selective sample preparation strategy proposed herein presented itself as a potential procedure to be employed to improve the accurate analysis of adulterated artificial sugar sweeteners that are usually illegally added to the so-called `100%` fruit juices by producers to improve their taste.

Keywords: `100%` fruit juice; food adulteration; selective isolation; selective pre-concentration; imprinted polymers; selective sample preparation


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About the article

Received: 2018-07-11

Accepted: 2018-07-12

Published Online: 2018-09-19

Published in Print: 2018-09-01

Citation Information: Molecular Imprinting, Volume 5, Issue 1, Pages 16–24, ISSN (Online) 2084-8803, DOI: https://doi.org/10.1515/molim-2018-0001.

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© by Hawa W. Mukami and Bareki S. Batlokwa, published by De Gruyter.Get Permission

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