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Nova Biotechnologica et Chimica

The Journal of University of SS. Cyril and Methodius

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CiteScore 2016: 0.42

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1338-6905
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Optimization of Barley Mature Embryo Regeneration and Comparison with Immature Embryos of Local Cultivars

Marcela Gubišová
  • Plant Production Research Institute Piešťany, Plant Production Research Center Piešťany, Bratislavská cesta 122, SK-921 68 Piešťany, Slovak Republic
  • Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nábrežie mládeže 91, SK-949 74 Nitra, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Daniel Mihálik
  • Plant Production Research Institute Piešťany, Plant Production Research Center Piešťany, Bratislavská cesta 122, SK-921 68 Piešťany, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jozef Gubiš
  • Plant Production Research Institute Piešťany, Plant Production Research Center Piešťany, Bratislavská cesta 122, SK-921 68 Piešťany, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2012-06-28 | DOI: https://doi.org/10.2478/v10296-012-0006-z

Optimization of Barley Mature Embryo Regeneration and Comparison with Immature Embryos of Local Cultivars

Regeneration ability of plant cells or tissues in explant culture is one of the key factors affecting success of genetic transformation. In experiments, the effect of explant type (whole embryo, scutellum embryonic axis, meristematic/central zone of embryonic axis) and plant growth regulators (BAP or TDZ) on mature embryo regeneration was determined. Explant type significantly affected regeneration efficiency. While no regenerants were observed using mature scutella, whole embryos or embryonic axes produced the highest number of regenerants. Using embryonic axes with discarded apical and basal parts, regeneration efficiency dramatically decreased. No statistical differences in regeneration were observed between BAP and TDZ added to the regeneration medium in concentration 0.1 or 1 mg l-1. At last, regeneration ability of mature embryos of nine Slovak spring barley cultivars (Donaris, Ezer, Levan, Ludan, Nitran, Pribina Sladar, Orbit, Pax) and Golden Promise as a model cultivar was examined and compared with regeneration ability of immature embryos which have been usually used for genetic transformation of barley. Although the regeneration from mature embryos was very weak, the same cultivars Golden Promise, Pribina and Levan showed the best regeneration ability by using both, immature and mature embryos. On the other hand cultivars Ezer and Pax belonged to the weakest ones in both experiments.

Keywords: BAP; barley regeneration; explant type; immature embryo; mature embryo; thidiazuron (TDZ)

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About the article


Published Online: 2012-06-28

Published in Print: 2012-06-01


Citation Information: Nova Biotechnologica et Chimica, ISSN (Print) 1338-6905, DOI: https://doi.org/10.2478/v10296-012-0006-z.

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