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Nova Biotechnologica et Chimica

The Journal of University of SS. Cyril and Methodius

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CiteScore 2016: 0.42

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1338-6905
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Actinomyces Ruminicola G10 - The Rumen Bacterium Recovered from Glycerol Enriched Cultivation Media

Anna Vandžurová
  • Corresponding author
  • Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, SK-040 01 Košice, Slovak Republic
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  • Other articles by this author:
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/ Gabriel Bódy
  • Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, SK-040 01 Košice, Slovak Republic
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  • De Gruyter OnlineGoogle Scholar
/ Peter Javorský
  • Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, SK-040 01 Košice, Slovak Republic
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/ Peter Pristaš
  • Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, SK-040 01 Košice, Slovak Republic
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Published Online: 2013-09-06 | DOI: https://doi.org/10.2478/nbec-2013-0004

Abstract

Gradual increasing of glycerol concentration up to 10% using sheep ruminal fluid as an inoculum for in vitro cultivation was accompanied by significant changes in bacterial population as documented by DGGE analysis. The resulting bacterial consortium was composed of three dominant bacteria with Actinomyces related bacterium to be predominant. Upon cultivation on media with glycerol as a sole carbon source a single bacterium was cultivated from this consortium. Isolate G10 was found to be anaerobic, Gram-positive rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequences showed that G10 isolate is related to the Actinomyces ruminicola species (97.7% of similarity). The role of rumen actinobacteria is largely unknown and their participation in glycerol utilization (tolerance) has not been described yet. The G10 bacterium and related consortium could be possibly used to improve glycerol tolerance and uptake by ruminants

Keywords : rumen bacteria; sheep; glycerol; Actinomyces ruminicola

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About the article

Published Online: 2013-09-06

Published in Print: 2013-06-01


Citation Information: Nova Biotechnologica et Chimica, ISSN (Print) 1338-6905, DOI: https://doi.org/10.2478/nbec-2013-0004.

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