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Nova Biotechnologica et Chimica

The Journal of University of SS. Cyril and Methodius

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CiteScore 2016: 0.42

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Application of Isotopic Dilution and Single-Step Extractions for Labile Soil Zinc Determination

Vladimír Frišták
  • Corresponding author
  • Department of Ecochemistry and Radioecology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Martin Pipíška
  • Department of Ecochemistry and Radioecology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Tatiana Gablovičová
  • Department of Ecochemistry and Radioecology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic
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  • De Gruyter OnlineGoogle Scholar
/ Juraj Lesný
  • Department of Ecochemistry and Radioecology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2013-09-06 | DOI: https://doi.org/10.2478/nbec-2013-0005


Concentration of available zinc from soils is the primary concern in assessment of its toxicity or essentiality for plants. This study evaluates the changes in chemical extractable Zn from three Slovak typical soils with simultaneous extractions as tools of zinc bioavailability. We found out that extractability of binding zinc decreased in order Na2EDTA, Mehlich 3, Mehlich 2, NH4NO3 and CaCl2 for all soil samples. Using flow-through stripping chronopotentiometry (SCP) and atomic absorption spectrometry (GFAAS) we found out that maximum of soil zinc was removed by organic ligands. Lability of Zn determined by isotopic dilution method using 65Zn and γ-spectrometry showed the significant decrease of isotopic exchangeable zinc fraction (E-value) with decrease of soil reaction. Obtained E-values of uppermost soil horizons showed the zinc lability ranged from 20 to 39%. Our research confirmed the effect of soil reaction, composition and physico-chemical characteristics to Zn lability. For further assessment of zinc bioavailability is needed to find the correlation and effects of structural changes and aging in studied soils

Keywords : soil; Zn; bioavailability; extraction; E-value

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About the article

Published Online: 2013-09-06

Published in Print: 2013-06-01

Citation Information: Nova Biotechnologica et Chimica, Volume 12, Issue 1, Pages 46–55, ISSN (Print) 1338-6905, DOI: https://doi.org/10.2478/nbec-2013-0005.

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