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Nova Biotechnologica et Chimica

The Journal of University of SS. Cyril and Methodius

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CiteScore 2016: 0.42

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Metagenomic Analysis of Slovak Bryndza Cheese Using Next-Generation 16S rDNA Amplicon Sequencing

Matej Planý
  • Corresponding author
  • Department of Biology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic
  • Department of Microbiology and Molecular Biology, Food Research Institute NAFC, Priemyselná 4, 824 75 Bratislava 26, Slovak Republic
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Tomáš Kuchta
  • Department of Microbiology and Molecular Biology, Food Research Institute NAFC, Priemyselná 4, 824 75 Bratislava 26, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Katarína Šoltýs / Tomáš Szemes / Domenico Pangallo
  • Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 845 51 Bratislava, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Peter Siekel
  • Department of Biology, University of SS. Cyril and Methodius, J. Herdu 2, Trnava, SK-917 01, Slovak Republic
  • Department of Microbiology and Molecular Biology, Food Research Institute NAFC, Priemyselná 4, 824 75 Bratislava 26, Slovak Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-08-04 | DOI: https://doi.org/10.1515/nbec-2016-0003


Knowledge about diversity and taxonomic structure of the microbial population present in traditional fermented foods plays a key role in starter culture selection, safety improvement and quality enhancement of the end product. Aim of this study was to investigate microbial consortia composition in Slovak bryndza cheese. For this purpose, we used culture-independent approach based on 16S rDNA amplicon sequencing using next generation sequencing platform. Results obtained by the analysis of three commercial (produced on industrial scale in winter season) and one traditional (artisanal, most valued, produced in May) Slovak bryndza cheese sample were compared. A diverse prokaryotic microflora composed mostly of the genera Lactococcus, Streptococcus, Lactobacillus, and Enterococcus was identified. Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris were the dominant taxons in all tested samples. Second most abundant species, detected in all bryndza cheeses, were Lactococcus fujiensis and Lactococcus taiwanensis, independently by two different approaches, using different reference 16S rRNA genes databases (Greengenes and NCBI respectively). They have been detected in bryndza cheese samples in substantial amount for the first time. The narrowest microbial diversity was observed in a sample made with a starter culture from pasteurised milk. Metagenomic analysis by high-throughput sequencing using 16S rRNA genes seems to be a powerful tool for studying the structure of the microbial population in cheeses.

Keywords: Lactic acid bacteria; Bryndza cheese; Next-generation sequencing; metagenomics; 16S rRNA genes


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About the article

Received: 2015-11-27

Accepted: 2016-05-05

Published Online: 2016-08-04

Published in Print: 2016-06-01

Citation Information: Nova Biotechnologica et Chimica, Volume 15, Issue 1, Pages 23–34, ISSN (Online) 1338-6905, DOI: https://doi.org/10.1515/nbec-2016-0003.

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© by Matej Planý. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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