Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Nukleonika

The Journal of Instytut Chemii i Techniki Jadrowej

4 Issues per year


IMPACT FACTOR 2016: 0.760

CiteScore 2016: 0.55

SCImago Journal Rank (SJR) 2015: 0.205
Source Normalized Impact per Paper (SNIP) 2015: 0.461

Open Access
Online
ISSN
0029-5922
See all formats and pricing
More options …

Trace elements in scalp hair of leukaemia patients

Ali Khuder
  • Corresponding author
  • Department of Chemistry, Atomic Energy Commission of Syria (AECS), P. O. Box 6091, Damascus, Syria, Tel.: +963 11 213 2580, Fax: +963 11 611 2289
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mohammad Adel Bakir
  • Department of Radiation Medicine, Atomic Energy Commission of Syria (AECS), P. O. Box 6091, Damascus, Syria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Reem Hasan
  • Department of Chemistry, Atomic Energy Commission of Syria (AECS), P. O. Box 6091, Damascus, Syria, Tel.: +963 11 213 2580, Fax: +963 11 611 2289
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ali Mohammad
  • Department of Radiation Medicine, Atomic Energy Commission of Syria (AECS), P. O. Box 6091, Damascus, Syria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Khozama Habil
  • Department of Radiation Medicine, Atomic Energy Commission of Syria (AECS), P. O. Box 6091, Damascus, Syria
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-09-12 | DOI: https://doi.org/10.2478/nuka-2014-0014

Abstract

The aim of this study was to determine the concentration of Fe, Ni, Cu, Zn and Pb in scalp hair of leukaemia patients and healthy volunteers, using the optimised XRF method. Leukaemia hair samples were classifi ed corresponding to type, growth and age of the participants. The results showed that the studied trace elements (TEs) in both of leukaemia and control groups were positively skewed. In comparison with the control group, lower Fe, Cu, Zn and Pb and higher of Ni medians were found in all studied leukaemia patients. The median rank obtained by Mann-Whitney U-test revealed insignifi cant differences between the leukaemia patients subgroups and the controls. An exact probability (α < 0.05) associated with the U-test showed signifi cant differences between medians in leukaemia patients and controls groups for Pb (lymphatic/control, acute/control), Cu (lymphatic/control, chronic/control), Ni (lymphatic/control, chronic/control) and Fe (chronic/control). Very strong positive and negative correlations (r > 0.70) in the scalp hair of control group were observed between Ni/Fe-Ni, Cu/Fe-Cu, Zn/Fe-Zn, Pb/Fe-Pb, Cu/Ni-Zn/Ni, Cu/Ni-Pb/Ni, Zn/Ni-Pb/Ni, Zn/Fe-Zn/Cu, Pb/Ni-Ni and Ni/Fe-Pb/Ni, whereas only very strong positive ratios in the scalp hair of leukaemia patients group were observed between Ni/Fe-Ni, Cu/Fe-Cu, Zn/Fe-Zn and Pb/Fe-Pb, all correlations were signifi cant at p < 0.05. Other strong and signifi cant correlations were also observed in scalp hair of both groups. Signifi cant differences between grouping of studied TEs in all classifi ed leukaemia groups and controls were found using principal component analysis (PCA). The results of PCA confi rmed that the type and the growth of leukaemia factors were more important in element loading than the age factor.

Keywords: leukaemia; principal component analysis; scalp hair; U-test; X-ray fl uorescence

References

  • 1. Al-Sayer, H., Mathew, T. C., Asfar, S., Khourshed, M., Al-Bader, A., Behbehani, A., & Dashti, H. (2004). Serum changes in trace elements during thyroid cancers. Mol. Cell. Biochem., 260, 1-5.Google Scholar

  • 2. Demir, C., Demir, H., Esen, R., Sehitogullari, A., Atmaca, M., & Alay, M. (2011). Altered serum levels of elements in acute leukemia cases in Turkey. Asian Pac. J. Cancer Prev., 12, 3471-3474.Google Scholar

  • 3. Fukuda, H., Ebara, M., Yamada, H., Arimoto, M., Okabe, S., Obu, M., Yoshikawa, M., Sugiura, N., & Saisho, H. (2004). Trace elements and cancer. Japan Medical Association Journal (JMAJ), 47, 391-395.Google Scholar

  • 4. Karimi, G,. Shahar, S., Homayouni, N., Rajikan, R., Abu Bakar, N. F., & Othman, M. S. (2012). Association between trace element and heavy metal levels in hair and nail with prostate cancer. Asian Pac. J. Cancer Prev., 13, 4249-4253.Google Scholar

  • 5. Kolmogorov, Y., Kovaleva, V., & Gonchar, A. (2000). Analysis of trace elements in scalp hair of healthy people, hyperplasia and breast cancer patients with XRF method. Nucl. Instrum. Methods Phys. Res. Sect. A: Accel. Spectrom. Dect. Assoc. Equip., 448, 457-460.Google Scholar

  • 6. Milde, D., Altmannova, K., Vysloužil, K., & Stužka, V. (2005). Trace element levels in blood serum and colon tissue in colorectal cancer. Chem. Pap., 59, 157-160.Google Scholar

  • 7. Tan, C., & Chen, H. (2011). Screening cancer by analyzing trace elements in hair and chemometrics. Biol. Trace Elem. Res., 144, 97-108.Web of ScienceGoogle Scholar

  • 8. Abdulrahman, F. I., Akan, J. C., Chellube, Z. M., & Waziri, M. (2012). Levels of heavy metals in human hair and nail samples from Maiduguri Metropolis, Borno State, Nigeria. World Environ., 2, 81-89.CrossrefGoogle Scholar

  • 9. Mehra, R., & Juneja, M. (2005). Elements in scalp hair and nails indicating metal body burden in polluted environment. J. Sci. Ind. Res., 64, 119-124.Google Scholar

  • 10. Hicks, J., Bartholomew, J., Ward-Smith, P., & Hutto, C. J. (2003). Quality of life among childhood leukemia patients. J. Pediatr. Oncol. Nurs., 20, 192-200.CrossrefGoogle Scholar

  • 11. Bass, D. A., Hickok, D., Quig, D., & Urek, K. (2001). Trace element analysis in hair: Factors determining accuracy, precision, and reliability. Altern. Med. Rev., 6, 472-481.Google Scholar

  • 12. Dressler, V. L., Pozebon, D., Mesko, M. F., Matuscvh, A., Kumtabtim, U., Wu, B., & Beckert, J. S. (2010). Biomonitoring of essential and toxic metals in single hair using on-line solution-based calibration in laser ablation inductively coupled plasma mass spectrometry. Talanta, 82, 1770-1777.Web of ScienceGoogle Scholar

  • 13. Gellein, K., Lierhagen, S., Brevik, P. S., Teigen, M., Kaur, P., Singh, T., Flaten, T. P., & Syversen, T. (2008). Trace element profi les in single strands of human hair determined by HR-ICP-MS. Biol. Trace Elem. Res., 123, 250-260.Web of ScienceGoogle Scholar

  • 14. Khuder, A., Bakir, M. A., Karjou, J., & Sawan, M. Kh. (2007). XRF and TXRF techniques for multi-element determination of trace elements in whole blood and human hair samples. J. Radioanal. Nucl. Chem., 273, 435-442.Web of ScienceGoogle Scholar

  • 15. Saiki, M., Alves, E. R., Jaluul, O., Sumita, N. M., & Filho, W. J. (2008). Determination of trace elements in scalp hair of an elderly population by neutron activation analysis. J. Radioanal. Nucl. Chem., 276, 53-57.Google Scholar

  • 16. Tadayon, F., Tehrani, M. S., & Nia, S. R. (2012). Determination of toxic and essential elements in the scalp hair of patients with type 2 diabetes. Acad. Res. Int., 2, 11-16.Google Scholar

  • 17. IAEA. (1997). Sampling, storage and sample preparation procedures for X ray fl uorescence analysis of environmental materials. Vienna: International Atomic Energy Agency. (IAEA-TECDOC-950).Google Scholar

  • 18. Khuder, A., Bakir, M. A., Solaiman, A., Issa, H., Habil, Kh., & Mohammad, A. (2012). Major, minor, and trace elements in whole blood of patients with different leukemia patterns. Nukleonika, 57(3), 389-399.Google Scholar

  • 19. IAEA. (2005). Quantitative X-ray analysis system. QXAS, Doc. Version 2.0. Vienna: International Atomic Energy Agency.Google Scholar

  • 20. Konieczka, P., & Namiesnik, J. (2009). Quality assurance and quality control in the analytical chemical laboratory: a practical approach. Boca Raton: CRC Press.Google Scholar

  • 21. Pasha, Q., Malik, S. A., & Shah, M. H. (2008). Statistical analysis of trace metals in the plasma of cancer patients versus controls. J. Hazard. Mater., 153, 1215-1221.Web of ScienceGoogle Scholar

  • 22. Weiss, C. H. (2007) StatSoft, Inc., Tusla, OK.STATISTICA Version 8.0. AStA Adv. Stat. Anal., 91, 339-341.Google Scholar

  • 23. Carvallo, M. L., Brito, J., & Barreiros, M. A. (1998). Study of trace element concentrations in human tissues by EDXRF spectrometry. X-Ray Spectrom., 27, 198-204.CrossrefGoogle Scholar

  • 24. Eltayeb, M. A. H., & Van Grieken, R. E. (1989). Preconcentration and XRF-determination of heavy metals in hair from Sudanese populations. J. Radioanal. Nucl. Chem., 131, 331-342.Google Scholar

  • 25. Khudzari, J. Md., Wagiran, H., Hossain, MdI., Ibrahim, N., & Agam, M. A. (2011). Heavy metals Mn, Fe, Ni, Cu and Zn in human hair samples using energy dispersive X-ray fl uorescence analysis. Int. J. Phys. Sci., 6, 2090-2094.Google Scholar

  • 26. Rao, K. S., Balaji, T., Rao, T. P., Babu, Y., & Naidu, G. R. K. (2002). Determination of iron, cobalt, nickel, manganese, zinc, copper, cadmium and lead in human hair by inductively coupled plasma atomic emission spectrometry. Spectroc. Acta Pt. B-Atom. Spectr., 57, 1333-1338.Google Scholar

  • 27. Sturaro, A., Parvoli, G., Doretti, L., Zanchetta, S., & Allegri, G. (1993). Simultaneous determination of trace metals in human hair by dynamic ion-exchange chromatography. Anal. Chim. Acta, 274, 163-170.Google Scholar

  • 28. Modaressi, A., Hadjibabaie, M., Shamshiri, A. R, Namdar, R., Abdollahi, M., & Ghavamzadeh, A. (2012). Trace elements (Se, Zn, and Cu) levels in patients with newly diagnosed acute leukemia. IJHOSCR, 6, 5-10.Google Scholar

  • 29. Sanaat, Z., Nouri, M., Hajipour, B., Dolatkhah, R., Asvadi, I., Vaez, J., Eivazi, J., Nikanfar, A., Esfahani, A., Chavoshi, Sh., & Biorani, H. (2011). Evaluation of copper, zinc, Cu/Zn, and VEGF in patients with AML in Iran. Iran. J. Cancer Prev., 4, 151-153.Google Scholar

About the article

Received: 2013-07-03

Accepted: 2014-06-13

Published Online: 2014-09-12

Published in Print: 2014-08-01


Citation Information: Nukleonika, Volume 59, Issue 3, Pages 111–120, ISSN (Online) 0029-5922, DOI: https://doi.org/10.2478/nuka-2014-0014.

Export Citation

© by Ali Khuder. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Comments (0)

Please log in or register to comment.
Log in