Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Oceanological and Hydrobiological Studies


IMPACT FACTOR 2017: 0.461
5-year IMPACT FACTOR: 0.604

CiteScore 2017: 0.64

SCImago Journal Rank (SJR) 2017: 0.249
Source Normalized Impact per Paper (SNIP) 2017: 0.463

Online
ISSN
1897-3191
See all formats and pricing
More options …
Volume 42, Issue 1

Issues

An improved method for efficient isolation and purification of genomic DNA from filamentous cyanobacteria belonging to genera Anabaena, Nodularia and Nostoc

Angelika Kaczyńska / Marcin Łoś / Grzegorz Węgrzyn
Published Online: 2013-04-12 | DOI: https://doi.org/10.2478/s13545-013-0058-y

Abstract

The aim of this work was to develop an improved method for isolation and purification of genomic DNA from filamentous cyanobacteria. The method described here employs a modified phenol extraction-based procedure. It allowed us to obtain a high yield (60–620 μg/g wet weight, depending on the cyanobacterial strain) of pure and undegraded genomic DNA (A260/A280 ratio of about 1.8 and A260/A230 ratio of about 2.0). Genomic DNA, isolated from cyanobacteria belonging to the genera Anabaena, Nodularia and Nostoc has been successfully used for construction of gene libraries. Thus, this method can be used in procedures requiring highly purified cyanobacterial DNA.

Keywords: DNA isolation; DNA purification; filamentous cyanobacteria; genomic DNA; genome library construction

  • [1] Bertozzini, E., Penna, A., Pierboni, E., Bruce, I.J. and Magnani, M. (2005) Development of new procedures for the isolation of phytoplankton DNA from fixed samples. J Appl Phycol 17, 223–229. http://dx.doi.org/10.1007/s10811-005-2130-5CrossrefGoogle Scholar

  • [2] Bollet, C., Gevaudan, M.J., de Lamballerie, X., Zandotti, C. and de Micco, P. (1991) A simple method for the isolation of chromosomal DNA from gram positive or acid-fast bacteria. Nucleic Acids Res 19, 1955. http://dx.doi.org/10.1093/nar/19.8.1955CrossrefGoogle Scholar

  • [3] Burja, A.M., Banaigs, B., Abou-Mansour, E., Burgess, J.G. and Wright, P.C. (2001) Marine cyanobacteria — a prolific source of natural products. Tetrahedron 57, 9347–9377. http://dx.doi.org/10.1016/S0040-4020(01)00931-0CrossrefGoogle Scholar

  • [4] Fiore, M.F., Moon, D.H., Tsai, S.M., Lee, H. and Trevors, J.T. (2000) Miniprep DNA isolation from unicellular and filamentous cyanobacteria. J Microbiol Meth 39, 159–169. http://dx.doi.org/10.1016/S0167-7012(99)00110-4CrossrefGoogle Scholar

  • [5] Hoiczyk, E. and Hansel, A. (2000) Cyanobacterial cell walls: news from an unusual prokaryotic envelope. J Bacteriol 182, 1191–1199. http://dx.doi.org/10.1128/JB.182.5.1191-1199.2000CrossrefGoogle Scholar

  • [6] Kennedy, J., Flemer, B., Jackson, S.A., Lejon, D.P.H., Morrissey, J.P., O’Gara, F. and Dobson, A.D.W. (2010) Marine metagenomics: new tools for the study and exploitation of marine microbial metabolism. Mar Drugs 8, 608–628. http://dx.doi.org/10.3390/md8030608Web of ScienceCrossrefGoogle Scholar

  • [7] Liberton, M. and Pakrasi, H.B. (2008) Membrane systems in cyanobacteria. In: The cyanobacteria: molecular biology, genomics and evolution eds. Herrero, A. and Flores, E., pp 277–285, Norwich: Caister Academic Press. Google Scholar

  • [8] Manchester, K.L. (1995) Value of A260/A280 ratios for measurement of purity of nucleic acids. Biotechniques 19, 208–210. Google Scholar

  • [9] Morin, N., Vallaeys, T., Hendrickx, L., Natalie, L. and Wilmotte, A. (2010) An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira. J Microbiol Meth 80, 148–154. http://dx.doi.org/10.1016/j.mimet.2009.11.012CrossrefWeb of ScienceGoogle Scholar

  • [10] Pope, P.B. and Patel, B.K.C. (2008) Metagenomic analysis of a freshwater toxic cyanobacteria bloom. FEMS Microbiol Ecol 64, 9–27. http://dx.doi.org/10.1111/j.1574-6941.2008.00448.xWeb of ScienceCrossrefGoogle Scholar

  • [11] Saha, S.K., Uma, L. and Subramanian, G. (2005) An improved method for marine cyanobacterial DNA isolation. World J Microbiol Biotechnol 21, 877–881. http://dx.doi.org/10.1007/s11274-004-6187-0CrossrefGoogle Scholar

  • [12] Sambrook, J. and Russell, D.W. (2001) Molecular cloning: a laboratory manual. 3rd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press. Google Scholar

  • [13] Sinha, S., Dadheech, P.K., KrishnaMo, M. (2009) Rapid DNA extraction from cyanobacteria. www.protocol-online.org. Date added: Feb 02, 2009 Google Scholar

  • [14] Sivonen, K. and Börner, T. (2008) Bioactive compounds produced by cyanobacteria. In: The cyanobacteria: molecular biology, genomics and evolution, eds. Herrero, A. and Flores, E. pp. 159–183, Norwich: Caister Academic Press. Google Scholar

  • [15] Sivonen, K., Leikoski, N., Fewer, D.P. and Jokela, J. (2010) Cyanobactins—ribosomal cyclic peptides produced by cyanobacteria. Appl Microbiol Biotechnol 86, 1213–1225. http://dx.doi.org/10.1007/s00253-010-2482-xCrossrefWeb of ScienceGoogle Scholar

  • [16] Wu, X., Zarka, A. and Boussiba, S. (2000) A simplified protocol for preparing DNA from filamentous cyanobacteria. Plant Mol Biol Rep 18, 385–392. http://dx.doi.org/10.1007/BF02825067CrossrefGoogle Scholar

About the article

Published Online: 2013-04-12

Published in Print: 2013-03-01


Citation Information: Oceanological and Hydrobiological Studies, Volume 42, Issue 1, Pages 8–13, ISSN (Online) 1897-3191, ISSN (Print) 1730-413X, DOI: https://doi.org/10.2478/s13545-013-0058-y.

Export Citation

© 2013 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Agnieszka Felczykowska, Aleksandra Dydecka, Małgorzata Bohdanowicz, Tomasz Gąsior, Marek Soboň, Justyna Kobos, Sylwia Bloch, Bożena Nejman-Faleńczyk, and Grzegorz Węgrzyn
Microbial Cell Factories, 2014, Volume 13, Number 1

Comments (0)

Please log in or register to comment.
Log in