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Pure and Applied Chemistry

The Scientific Journal of IUPAC

Ed. by Burrows, Hugh / Stohner, Jürgen


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1365-3075
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Volume 84, Issue 6

Issues

Systematic isolation of microbial metabolites for natural products depository (NPDepo)

Hiroyuki Osada
  • Corresponding author
  • Chemical Biology Core Facility, Chemical Biology Department, RIKEN Advanced Science Institute, Saitama 351-0198, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Toshihiko Nogawa
  • Corresponding author
  • Chemical Biology Core Facility, Chemical Biology Department, RIKEN Advanced Science Institute, Saitama 351-0198, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2011-12-08 | DOI: https://doi.org/10.1351/PAC-CON-11-08-11

A microbial fraction library has been constructed as a part of RIKEN Natural Products Depository (NPDepo) to discover and isolate novel metabolites with unique biological activity from microbial sources efficiently and rapidly. The fraction library was made by a systematic separation method based on basic chromatographic techniques. Each fraction in the library was analyzed by liquid chromatography/mass spectrometry (LC/MS) to reveal physicochemical properties of each metabolite within the fraction, and the results were applied to construct a database for rapid discovery of novel and structurally unique compounds. We developed a new type of metabolite database called MP (microbial products) plot to visualize each metabolite on a 2D area. The combination of the fraction library and the database led to the discovery and isolation of novel metabolites, verticilactam, which was a 16-membered macrolactam with an unprecedented β-keto-amide moiety, and spirotoamides A and B, which had a highly substituted 6,6-spiroaccetal moiety and a carboxamide moiety. Moreover, based on the utilization of the MP plot, the new capacity of a streptomycete to produce specific metabolites was discovered.

Keywords: biosynthesis; fraction library; mass spectrometry; microbial products; MP plot; natural products; spectral database; systematic isolation; UV–vis spectroscopy

Conference

International Symposium on the Chemistry of Natural Products and the 7th International Conference on Biodiversity (ISCNP-27 & ICOB-7), International Conference on Biodiversity, International Symposium on the Chemistry of Natural Products, ICOB, ISCNP, Biodiversity, Natural Products, 27th, Brisbane, Australia, 2011-07-10–2011-07-15

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About the article

Published Online: 2011-12-08

Published in Print: 2011-12-08


Citation Information: Pure and Applied Chemistry, Volume 84, Issue 6, Pages 1407–1420, ISSN (Online) 1365-3075, ISSN (Print) 0033-4545, DOI: https://doi.org/10.1351/PAC-CON-11-08-11.

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