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Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

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Usefulness of PCR/RFLP and ERIC PCR techniques for epidemiological study of Haemophilus parasuis infections in pigs

A. Jabłoński
  • Swine Diseases Department, National Veterinary Research Institute, 57 Partyzantow Str., 24-100 Pulawy, Poland
/ S. Zębek
  • Swine Diseases Department, National Veterinary Research Institute, 57 Partyzantow Str., 24-100 Pulawy, Poland
/ R. Kołacz
  • Department of Genetics and Animal Breeding, Wroclaw University of Environmental and Life Sciences, 25/27 C.K. Norwida Str., 50-375 Wroclaw, Poland
/ Z. Pejsak
  • Swine Diseases Department, National Veterinary Research Institute, 57 Partyzantow Str., 24-100 Pulawy, Poland
Published Online: 2011-08-02 | DOI: https://doi.org/10.2478/v10181-011-0016-9

Usefulness of PCR/RFLP and ERIC PCR techniques for epidemiological study of Haemophilus parasuis infections in pigs

Haemophilus parasuis belongs to opportunistic microorganisms of undefined virulence. The purpose of the studies was to compare suitability of PCR/RFLP in our modification and ERIC PCR for epidemiological study of domestic strains of H. parasuis. The results were evaluated taking into account two different aspects: suitability of the tests for isolating the highest possible number of clone groups and subjective evaluation of the method judged with respect to the following criteria: difficulty, availability of equipment and reagents as well as time and cost of the study. The results obtained in the present study show that the two methods used for typing of H. parasuis had high discriminatory power. Taking into account this parameter it can be concluded that ERIC PCR is more suitable than PCR/RFLP. This justifies the use of ERIC PCR for routine epidemiological analyses of mentioned pathogen. Taking into account the complexity of method used, ERIC-PCR based on random amplification of DNA, proved to be comparable to PCR/RFLP. The last mentioned technique is relatively less expensive and labour-consuming, especially when diagnostic PCR method is used for the epidemiological studies.

Keywords: Haemophilus parasuis; PCR/RFLP; ERIC PCR; genotyping

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Published Online: 2011-08-02

Published in Print: 2011-12-01

Citation Information: Polish Journal of Veterinary Sciences. Volume 14, Issue 1, Pages 111–116, ISSN (Print) 1505-1773, DOI: https://doi.org/10.2478/v10181-011-0016-9, August 2011

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