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Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

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2300-2557
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The experimental osteoporosis in sheep – clinical approach

Z. Kiełbowicz
  • Department and Surgery Clinic, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wrocław, Poland
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/ A. Piątek
  • Corresponding author
  • Department and Surgery Clinic, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wrocław, Poland
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/ J. Bieżyński
  • Department and Surgery Clinic, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wrocław, Poland
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/ P. Skrzypczak
  • Department and Surgery Clinic, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wrocław, Poland
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/ P. Kuropka
  • Department of Biostructure and Animal Physiology, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wrocław, Poland
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/ J. Kuryszko
  • Department of Biostructure and Animal Physiology, Faculty of Veterinary Medicine, University of Environmental and Life Sciences in Wrocław, Poland
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/ A. Nikodem
  • Department of Biomedical Engineering and Experimental Mechanics, Wrocław University of Technology
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/ P. Kafarski
  • Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Technology, 50-370 Wroclaw, Poland
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/ C. Pezowicz
  • Department of Biomedical Engineering and Experimental Mechanics, Wrocław University of Technology
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Published Online: 2015-11-27 | DOI: https://doi.org/10.1515/pjvs-2015-0083

Abstract

The implementation of new methods of oseoporotic therapy requires tests on an animal model. One of the best is the sheep, whose numerous advantages over other models are described in the literature. The aim of this study was induction of osteoporosis using steroids and ovariorectomy methods in sheep and description of the change in parameters with regard to healthy sheep. The study was performed on female „merino” breed sheep divided into three groups: Negative control (NC) healthy animals, positive control (PC) ovariorectomised animals and steroid group (SC) where methylprednisolone was implemented. Blood tests, diagnostic arthroscopy, quantitative computed tomography and X-Ray micro-tomography of bone were carried out. Blood tests revealed a decreased level of estrogens, progesterone and increased parathormone and cortisol levels in the SC group. A decrease in bone turnover markers and an increase in bone resorption markers in all groups were also noted. Diagnostic arthroscopy revealed osteoarthrosis in PC and SC groups. Radiological density tests showed a slight decrease in PC and NC groups whereas there was more than a triple decrease in SC. Results obtained from microCT showed quickly developing osteoporosis in the SC group, which is reflected in numerous parameters analysed in this study. The best effects for osteoporosis induction were obtained using ovariorectomised sheep with methylprednisolone injections.

Keywords: osteoporosis; animal model; indentation; mapping of elements; mechanical examinations; glucocorticosteroids

References

  • An YH, Gruber HE (2003) Introduction to Experimental Bone and Cartilage Histology. In: An YH, Martin KL (eds) Handbook of Histology Methods for Bone and Cartilage, 1st ed., Totowa, NJ, Human Press Inc, pp 3-31.Google Scholar

  • Canas CA, Osorio JC, Coronel N, Cepeda CM, Izquierdo HJ, Bonill-Abadia F (2014) Efficacy and safety of oral low-dose glucocorticoids in patients with estrogen-dependent primary osteoarthritis. Rheumatol Int 34: 733-735.Web of ScienceCrossrefGoogle Scholar

  • de Ridder CM, Delemarre-van de Waal HA (1998) Clinical utility of markers of bone turnover in children and. Curr Opin Pediatr 10: 441-448.CrossrefGoogle Scholar

  • Gajewska J, Ambroszkiewicz J, Laskowska-Klita T (1999) Biochemical markers of bone turnover and their use. Med Wieku Rozwoj 3: 421-432.Google Scholar

  • Hacker SA, Suh JK, Marui T, Woo SL (1992) Compressive properties of the human meniscus. Trans Orthop Res 17: 627.Google Scholar

  • Lill CA, Fluegel AK, Schneider E (2002) Effect of ovariectomy, malnutrition and glucocorticoid application on bone properties in sheep: a pilot study. Osteoporosis Int 13: 480-486.CrossrefGoogle Scholar

  • Newman E, Turner AS, Wark JD (1995) The potential of sheep for the study of osteopenia: current status and comparison with other animals models. Bone 16: 277-284.CrossrefGoogle Scholar

  • Newton J, Cooper RC, Gilbert JA, Johnson RB, Zardiachas LD (2004) The ovariectomized sheep as a model for human bone loss. J Comp Path 130: 323-326.Google Scholar

  • Oheim R, Amling M, Ignatius A, Pogoda P (2012) Large animal model for osteoporosis in humans: the ewe. Eur Cell Mater 24: 372-385.Google Scholar

  • Peichl P, Griesmacher A, Marteau R, Hejc S, Kumpan W, Muller MM, Broll H (2001) Serum crosslaps in comparison to serum osteocalcin and urinary bone resorption markers. Clin Biochem 34: 131-139.CrossrefGoogle Scholar

  • Proctor CS, Schmidt MB, Whipple RR, Kelly MA, Mow VC (1989) Material properties of the normal medial bovine meniscus. J Orthop Res 7: 771-782.CrossrefGoogle Scholar

  • Rabiega D, Woźniak J, Olejniczak T, Opala T (2000) Osteoporosis – epidemiology, pathogenesis, diagnostics and treatment. Przew Lek 9: 39-46.Google Scholar

  • Roman-Bias JA, Castaneda S, Largo R, Herrero-Beaumont G (2009) Osteoarthritis associated with estrogen deficiency. Arthritis Res Ther 11: 241.CrossrefGoogle Scholar

  • Schorlemmer S, Gohl Ch, Iwabu S, Ignatius A, Claes Lutz, Augat P (2003) Glucocorticoid Treatment of Ovariectomized Sheep Affects Mineral Density, Structure, and Mechanical Properties of Cancellous Bone. Bone Miner Res 18: 1010-1015.CrossrefGoogle Scholar

  • Sigrist IM, Gerhardt C, Alini M, Schneider E, Egermann M (2007) The long-term effects of ovariectomy on bone metabolism in sheep. J Bone Miner Metab 25: 28-35.CrossrefGoogle Scholar

  • Sweigart MA, Athanasiou KA (2004) Biomechanical characteristics of the normal medial and lateral porcine knee menisci. Proc I Mech E 219 Part H: JEngineering in Medicine.Google Scholar

  • Turner AS, Alvis M, Myers W, Stevens ML, Lundy MW (1995) Changes in bone mineral density and bone-alkaline phosphatase in ovariectomized ewes. Bone 17: 395-402.CrossrefGoogle Scholar

  • Turner AS (2002)The sheep as a model for osteoporosis in humans. The Veterinary Journal 163: 232-239.CrossrefGoogle Scholar

  • Uklejewski R, Winiecki M, Rogala P (2006) Zgodność strukturalno-adaptacyjna połączeń kości z porowatymi implantami na podstawie tradycyjnego jednofazowego I współczesnego dwufazowego porosprężystego modelu biomechaniczngo tkanki kostnej. Engineering of Biomaterials 1-13: 54-55Google Scholar

About the article

Published Online: 2015-11-27

Published in Print: 2015-09-01


Citation Information: Polish Journal of Veterinary Sciences, ISSN (Online) 2300-2557, DOI: https://doi.org/10.1515/pjvs-2015-0083.

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© 2015 Polish Academy of Sciences, Committee of Veterinary Sciences & University of Warmia and Mazury in Olsztyn. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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