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Photonics & Lasers in Medicine


SCImago Journal Rank (SJR) 2018: 0.162
Source Normalized Impact per Paper (SNIP) 2018: 0.346

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High-resolution two-photon imaging of HE-stained samples in dermatohistopathology – A pilot study on skin tumours

Hochauflösende Zwei-Photonen-Bildgebung von H&E-Schnitten in der Dermatohistopathology – Eine Pilotstudie an Hauttumoren

Daniela Göppner
  • Department of Dermatology and Venerology, Medical Faculty Magdeburg, Otto-v.-Guericke University of Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany
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/ Norma Mechow
  • Department of Dermatology and Venerology, Medical Faculty Magdeburg, Otto-v.-Guericke University of Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany
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  • De Gruyter OnlineGoogle Scholar
/ Julia Liebscher / Erik Thiel / Gunter Seewald / Anja Buchholz / Harald Gollnick
  • Department of Dermatology and Venerology, Medical Faculty Magdeburg, Otto-v.-Guericke University of Magdeburg, Leipziger Str. 44, 39120 Magdeburg, Germany
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/ Carsten M. Philipp
  • Department of Laser Medicine, Ev. Elisabeth Hospital, Lützowstr. 24–26, 10785 Berlin, Germany
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/ Karl-Heinz Schönborn

Abstract

Non-invasive two-photon microscopy (TPM) provides a new technique which could become in the future a substitute for hematoxylin and eosin (HE) staining of tissue sections of the epidermis and upper and middle dermis as well. High-resolution imaging, in combination with false-colour representation, allows an accurate reproduction of standard microscopy. The vertical skin viewing of epidermis and upper dermis by means of TPM allows for a new histopathological supportive technique, especially in non-melanoma skin cancer such as squamous cell carcinoma and basal cell carcinoma. If it could be used on fresh tissue samples, it could provide an alternative method to Mohs 3D histology, even though the definitive criteria for melanocytic tumours have not yet been sufficiently evaluated.

Zusammenfassung

Die neue, nicht-invasive Zwei-Photonen-Mikroskopie (two-photon microscopy, TPM) ermöglicht eine qualitativ hohe Reproduktion fast gleichwertig zu histologischen Präparaten exzidierter Tumoren, die die Epidermis und obere und mittlere Dermis erfassen. Mit Hilfe der durch die Scanner-Technik erzeugten hochaufgelösten Bilder in Kombination mit einer zusätzlichen Falschfarbendarstellung können relevante diagnostische wie prognostische Kriterien akkurat beurteilt werden. Die vertikale Bildgebung von Dermis und Epidermis durch die TPM ermöglicht eine ergänzende histopathologische Beurteilung vor allem bei weißem Hautkrebs wie dem Plattenepithel- oder Basalzellkarzinom. Ihr zukünftiger Einsatz an Frischpräparaten verspricht daher eine Alternative zu Mohs 3D-Histologie zu sein, auch wenn entscheidende Diagnosekriterien bei melanozytären Tumoren bislang noch nicht hinreichend beurteilbar sind.

Keywords: basal cell carcinoma; dermatohistopathology; ex-vivo imaging; false-colour representation; malignant melanoma; non-melanoma skin cancer; squamous cell carcinoma; two-photon microscopy; zoom function; Zwei-Photonen-Mikroskopie; Ex-vivo-Bildgebung; Übersicht- und Detailaufnahmen; Falschfarbendarstellung; Dermatohistologie; Plattenepithelkarzinom; Basalzellkarzinom; weißer Hautkrebs; malignes Melanom

About the article

Corresponding author


Received: 2011-10-21

Revised: 2012-03-09

Accepted: 2012-03-15

Published in Print: 2012-05-01


Citation Information: Photonics & Lasers in Medicine, Volume 1, Issue 2, Pages 133–140, ISSN (Online) 2193-0643, ISSN (Print) 2193-0635, DOI: https://doi.org/10.1515/plm-2012-0008.

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[1]
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[3]
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Optik & Photonik, 2015, Volume 10, Number 5, Page 39
[4]
C Czekalla, K H Schönborn, S Markworth, M Ulrich, D Göppner, H Gollnick, J Röwert-Huber, M E Darvin, J Lademann, and M C Meinke
Laser Physics Letters, 2015, Volume 12, Number 8, Page 085602
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Ming Ni and Shuangmu Zhuo
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