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Polish Polar Research

The Journal of Committee on Polar Research of Polish Academy of Sciences

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Volume 36, Issue 1


Isolation, identification and screening of Actinobacteria in volcanic soil of Deception Island (the Antarctic) for antimicrobial metabolites

Yoke−Kqueen Cheah
  • Corresponding author
  • Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Learn−Han Lee
  • Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Cheng−Yun Catherine Chieng
  • Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Vui−Ling Clemente Michael Wong
  • Biotechnology Research Institute, Universiti Malaysia Sabah, Locked Bag 2073, 88999 Kota Kinabalu, Sabah, Malaysia
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  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-03-25 | DOI: https://doi.org/10.1515/popore-2015-0001


This project aimed to isolate and characterize volcanic soil Actinobacteria from Deception Island, Antarctic. A total of twenty−four Actinobacteria strains were isolated using four different isolation media (Starch casein agar, R2 agar, Actinomycete isolation agar, Streptomyces agar) and characterized basing on 16S rRNA gene sequences. Tests for second− arymetabolites were performed using well diffusion method to detect antimicrobial activities against eight different pathogens, namely Staphyloccocus aureus ATCC 33591, Bacillus megaterium, Enterobacter cloacae, Klebsiella oxytoca, S. enterica serotype Enteritidis, S. enterica serotype Paratyphi ATCC 9150, S. enterica serotype Typhimurium ATCC 14028 and Vibrio cholerae. Antimicrobial properties were detected against Salmonella paratyphi A and Salmonella typhimurium at the concentration of 0.3092±0.08 g/ml. The bioactive strains were identified as Gordonia terrae, Leifsonia soli and Terrabacter lapilli. Results from this study showed that the soil of Deception Island is likely a good source of isolation for Actinobacteria. The volcanic soil Actinobacteria are potentially rich source for discovery of antimicrobial compounds.

Keywords: Antarctic; Actinobacteria; secondary metabolites; 16S; diffusion assay; selective isolation media


  • ARENSKOTTER M., BROKER D. and STEINBUCHEL A. 2004. Biology of the metabolically diverse ge− nus Gordonia. Applied and Environmental Microbiology 70: 3195-3204.Google Scholar

  • CHUN J., LEE J.H., JUNG Y., KIM M., KIM S., KIM B.K. and LIM Y.W. 2007. EzTaxon: a web−based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Interna− tional Journal of Systematic and Evolutionary Microbiology 57: 2259-2261.Google Scholar

  • DECEPTION ISLAND MANAGEMENT GROUP 2005. http://www.deceptionisland.aq/volcanic.php. Accessed 15 March 2010.Google Scholar

  • DRANCOURT M.,MCNEIL M.M., BROWN J.M., LASKER B.A.,MAURIN M., CHOUX M. and RAOUX D. 1994. Brain abscess due to Gordona terrae in an immunocompromised child: case report and review of infections caused by G. terrae. Clinical Infectious Disease 19: 258-262.Google Scholar

  • DU TOIT E.A. and RAUTENBACH M. 2000. A sensitive standardised micro−gel well diffusion assay for the determination of antimicrobial activity. Journal of Microbiology Methods 42: 159-165.Google Scholar

  • GRAMMER A. 1976. Antibiotic sensitivity and assay test. In: C.H. Collins and P.N. Lyne (eds) Micro− biological Methods Butterworths, London: 235 pp.Google Scholar

  • GRISOLD A.J.,ROLL P.,HOENIGL M., FEIERL G.,VICENZI−MOSER R. andMARTH E. 2007. Isolation of Gordonia terrae from a patient with catheter−related bacteraemia. Journal of Medical Micro− biology 56: 1687-1688.Web of ScienceGoogle Scholar

  • KUBOTA K., KOMA D.,MATSUMIYA Y., CHUNG S.−Y. and KUBO M. 2008. Phylogenetic analysis of long−chain hydrocarbon−degrading bacteria and evaluation of their hydrocarbon−degradation by the 2,6−DCPIP assay. Biodegradation 19: 749-757.CrossrefPubMedWeb of ScienceGoogle Scholar

  • LABEDA D.P. and SHEARER M.C. 1990. Isolation of actinomycetes for biotechnological applica− tions. In: D.P. Labeda (ed.) Isolation of Biotechnological Organisms from Nature. McGraw−Hill Inc, New York: 1-19.Google Scholar

  • LAZZARINI A., CAVALETTI L., TOPPO G. and MARINELLI F. 2000. Rare genera of actinomycetes as potential producers of new antibiotics. Antonie van Leeuwenhoek 78: 399-405.PubMedGoogle Scholar

  • LEE J.E., SEO J.P., LEE D.W.,KO Y.H. and LEE S.D. 2008. Terrabacter lapilli sp. nov., an actinomycete isolated from stone. International Journal of Systematic and Evolutionary Microbiology 58: 1084-1088.Web of ScienceGoogle Scholar

  • MADHAIYAN M., POONGUZHALI S., LEE J.S., SENTHILKUMAR M., LEE K.C. and SUNDARAM S. 2010. Leifsonia soli sp. nov., a novel yellow pigmented actinobacterium isolated from teak rhizosphere soil. International Journal of Systematic and Evolutionary Microbiology 60: 1322-1327.Web of ScienceGoogle Scholar

  • OKORO C.K., BROWN R., JONES A.L., ANDREWS B.A., ASENJO J.A., GOODFELLOW M. and BULL A.T. 2009. Diversity of culturable actinomycetes in hyper−arid soils of the Atacama Desert, Chile. Antonie van Leeuwenhoek 95: 121-133.PubMedGoogle Scholar

  • SANTELLA R.M. 2006. Approaches to DNA/RNA extraction and whole genome amplification. Can− cer Epidemiology, Biomarkers and Prevention 15: 1585-1587.Google Scholar

  • SHEN F.T., HO M.J., HUANG H.R., ARUN A.B., REKHA P.D. and YOUNG C.C. 2008. Molecular de− tection and phylogenetic characterization of Gordonia species in heavily oil−contaminated soils. Research in Microbiology 159: 522-529.Web of ScienceGoogle Scholar

  • SOLANO G., ROJAS−JIMENEZ K., JASPARS M. and TAMAYO−CASTILLO G. 2009. Study of the diver− sity of culturable actinomycetes in the North Pacific and Caribbean coasts of Costa Rica. Antonie van Leeuwenhoek 96: 71-78.Web of ScienceGoogle Scholar

  • SRIVIBOOL R. and SUKCHOTIRATANA M. 2006. Bioperspective of actinomycetes isolates from coastal soils: a new source of antimicrobial producers. Songklanakarin Journal of Science and Technology 28: 493-499.Google Scholar

  • STACKEBRANDT E., SMIDA J. and COLLINS M.D. 1988. Evidence of phylogenetic heterogeneity within the genus Rhodococcus: revival of the genus Gordona (Tsukamura). Journal of General and Applied Microbiology 34: 341-348.Google Scholar

  • THAKUR D., YADAV A., GOGOI B.K. and BORA T.C. 2007. Isolation and screening of Streptomyces in soil of protected forest areas from the states of Assam and Tripura, India, for antimicrobial metabolites. Journal of Medical Mycology 17: 242-249.CrossrefGoogle Scholar

  • TSUKAMURA M. 1971. Proposal of a new genus, Gordona, for slightly acid−fast organisms occurring in sputa of patients with pulmonary disease and in soil. Journal of General Microbiology 68: 15-26.Google Scholar

  • ZARDAWI I.M., JONES F., CLARK D.A. and HOLLAND J. 2004. Gordonia terrae induced suppurative granulomatous mastitis following nipple piercing. Pathology 36: 275-280. CrossrefPubMedGoogle Scholar

About the article

Received: 2011-06-02

Accepted: 2013-05-15

Published Online: 2015-03-25

Published in Print: 2015-03-01

Citation Information: Polish Polar Research, Volume 36, Issue 1, Pages 67–78, ISSN (Online) 2081-8262, DOI: https://doi.org/10.1515/popore-2015-0001.

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© Polish Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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