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Physical Sciences Reviews

Ed. by Giamberini, Marta / Jastrzab, Renata / Liou, Juin J. / Luque, Rafael / Nawab, Yasir / Saha, Basudeb / Tylkowski, Bartosz / Xu, Chun-Ping / Cerruti, Pierfrancesco / Ambrogi, Veronica / Marturano, Valentina / Gulaczyk, Iwona

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Micro-Raman spectroscopy in medicine

Christoph Krafft / Jürgen Popp
  • Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745 Jena, Germany
  • Institute of Physical Chemistry and Abbe Center of Photonics, University Jena, Helmholtzweg 4, 07743 Jena, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2019-08-30 | DOI: https://doi.org/10.1515/psr-2017-0047


A potential role of optical technologies in medicine including micro-Raman spectroscopy is diagnosis of bacteria, cells and tissues which is covered in this chapter. The main advantage of Raman-based methods to complement and augment diagnostic tools is that unsurpassed molecular specificity is achieved without labels and in a nondestructive way. Principles and applications of micro-Raman spectroscopy in the context of medicine will be described. First, Raman spectra of biomolecules representing proteins, nucleic acids, lipids and carbohydrates are introduced. Second, microbial applications are summarized with the focus on typing on species and strain level, detection of infections, antibiotic resistance and biofilms. Third, cytological applications are presented to classify single cells and study cell metabolism and drug–cell interaction. Fourth, applications to tissue characterization start with discussion of lateral resolution for Raman imaging followed by Raman-based detection of pathologies and combination with other modalities. Finally, an outlook is given to translate micro-Raman spectroscopy as a clinical tool to solve unmet needs in point-of-care applications and personalized treatment of diseases.

Keywords: Biomedical spectroscopy; biophotonics; microbiology; cytology; histopathology


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Published Online: 2019-08-30

Citation Information: Physical Sciences Reviews, Volume 4, Issue 10, 20170047, ISSN (Online) 2365-659X, DOI: https://doi.org/10.1515/psr-2017-0047.

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