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Pteridines

Official Journal of the International Society of Pteridinology

Editor-in-Chief: Fuchs, Dietmar


IMPACT FACTOR 2018: 0.531

CiteScore 2018: 0.67

SCImago Journal Rank (SJR) 2018: 0.195
Source Normalized Impact per Paper (SNIP) 2018: 0.318

ICV 2018: 145.86

Open Access
Online
ISSN
2195-4720
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Volume 6, Issue 3

Issues

Functional Studies on the Promoter Region of the Human GTP Cyclohydrolase I Gene

Markus Gütlich
  • Corresponding author
  • GSF-Institut für Klinische Molekularbiologie, Marchioninistraße 25, 81377 Müchen, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Klaus Witter / Gerd Katzenmeier / Wolfgang Rödl / Thomas Wernert / Irmgard Ziegler / Adelbert Bacher
  • Technische Universität München, Lehrstuhl fur Organische Chemie und Biochemie, 85747 Garching, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2013-05-03 | DOI: https://doi.org/10.1515/pteridines.1995.6.3.108

Summary

We have isolated genomic clones that contained the gene for human GTP cyclohydrolase I. One clone containing the 5'-regulatory region of this gene was further analysed. It encompassed the first exon, parts of the first intron and about 2.6kb of the promoter region. The transcription start site was localised by rapid amplification of cDNA ends (5'-RACE). The 2.6 kb region upstream of the transcription start site showed promoter activity when ligated upstream of a reporter gene. 5'-truncations of the promoter region increased its activity as long as the CAAT and TATA boxes remained unchanged. Several putative Spl responsive elements were located within a GC-rich region close to the transcription start site.

: Keywords: GTP cyclohydrolase I; Promoter; 5'-RACE; Spl

About the article

Published Online: 2013-05-03

Published in Print: 1995-08-01


Citation Information: Pteridines, Volume 6, Issue 3, Pages 108–111, ISSN (Online) 2195-4720, ISSN (Print) 0933-4807, DOI: https://doi.org/10.1515/pteridines.1995.6.3.108.

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