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Reviews in Analytical Chemistry

Editor-in-Chief: Schechter, Israel

Editorial Board Member: Pauw, Edwin / Vries, Mattanjah / Grushka, Eli / Laserna, J. / Licht, Stuart / Lubman, David / Mandler, Daniel / Palleschi, Vincenzo / Sigman, Michael / Whitesides, George

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Volume 30, Issue 1 (Apr 2011)


Volume 36 (2017)

Analysis of clenbuterol residues in pig liver using liquid chromatography electrospray ionization tandem mass spectrometry

Jelka Pleadin
  • Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, 10 000 Zagreb, Croatia
  • Email:
/ Igor Bratoš
  • Pliva Research Institute, Prilaz Baruna Filipovića 29, 10 000 Zagreb, Croatia
/ Ana Vulić
  • Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, 10 000 Zagreb, Croatia
/ Nina Perši
  • Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, 10 000 Zagreb, Croatia
/ Jelena Ðugum
  • Ministry of Agriculture, Fisheries and Rural Development, Grada Vukovara 78, 10 000 Zagreb, Croatia
Published Online: 2011-04-12 | DOI: https://doi.org/10.1515/revac.2011.008


The liquid chromatography-electrospray ionization tandem mass spectrometry method was developed for determination of clenbuterol residues in liver tissue as a regulatory matrix for the control of clenbuterol abuse as an anabolic. To investigate the level of clenbuterol residues during the withdrawal period, male food-producing pigs were exposed to subchronic repeat oral administration of a clenbuterol growth-promoting dose for 28 days. The analytical procedure showed acceptable validation results for all liver spiked samples analyzed and proved to be useful as a quantification and confirmation method in supporting regulatory enforcement programs of clenbuterol misuse monitoring. The highest level of clenbuterol in the liver of treated animals was recorded on day 0 of treatment cessation (21.58±14.29 ng/g), followed by 6.59±3.11 ng/g on day 3, 0.83±0.27 ng/g on day 7, and 0.44±0.08 ng/g on day 14 of withdrawal. At the end of the study period (day 35), the concentration of clenbuterol was below the method limit of detection (<0.1 ng/g).

Keywords: clenbuterol residues; liquid chromatography-electrospray ionization tandem mass spectrometry; liver; pigs

About the article

Published Online: 2011-04-12

Published in Print: 2011-04-01

Citation Information: Reviews in Analytical Chemistry, ISSN (Online) 2191-0189, ISSN (Print) 0793-0135, DOI: https://doi.org/10.1515/revac.2011.008. Export Citation

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