Reviews in Analytical Chemistry
Editor-in-Chief: Schechter, Israel
Editorial Board Member: Pauw, Edwin / Vries, Mattanjah / Grushka, Eli / Laserna, J. / Licht, Stuart / Lubman, David / Mandler, Daniel / Palleschi, Vincenzo / Sigman, Michael / Whitesides, George
IMPACT FACTOR 2015: 1.378
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Impact per Publication (IPP) 2015: 1.304
Top-down proteomic identification of bacterial protein biomarkers and toxins using MALDI-TOF-TOF-MS/MS and post-source decay
1Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA
Citation Information: Reviews in Analytical Chemistry. Volume 32, Issue 2, Pages 127–133, ISSN (Online) 2191-0189, ISSN (Print) 0793-0135, DOI: https://doi.org/10.1515/revac-2012-0035, April 2013
- Published Online:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a new tool for rapid identification of bacterial microorganisms. With the development of matrix-assisted laser desorption/ionization time-of-flight-time-of-flight (MALDI-TOF-TOF) instruments, new capabilities for characterizing bacteria and their protein biomarkers and toxins by top-down proteomic identification is now possible. A number of researchers have demonstrated top-down proteomic identification of nondigested proteins using MALDI-TOF-TOF platforms. The current review briefly discusses the use of MS instrumentation for bacterial identification and characterization as well as recent developments in the use of MALDI-TOF-TOF for top-down proteomic identification of protein biomarkers and toxins from unfractionated bacterial cell lysates. This approach provides great promise as a rapid method for identification and characterization of pathogenic microorganisms and their virulence factors.
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