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Reviews in Analytical Chemistry

Editor-in-Chief: Schechter, Israel

Editorial Board Member: Pauw, Edwin / Vries, Mattanjah / Grushka, Eli / Laserna, J. / Licht, Stuart / Lubman, David / Mandler, Daniel / Palleschi, Vincenzo / Sigman, Michael / Whitesides, George


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Top-down proteomic identification of bacterial protein biomarkers and toxins using MALDI-TOF-TOF-MS/MS and post-source decay

1Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA

Corresponding author: Clifton K. Fagerquist, Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, 800 Buchanan Street, Albany, CA 94710, USA

Citation Information: Reviews in Analytical Chemistry. Volume 32, Issue 2, Pages 127–133, ISSN (Online) 2191-0189, ISSN (Print) 0793-0135, DOI: https://doi.org/10.1515/revac-2012-0035, April 2013

Publication History

Received:
2012-11-07
Accepted:
2013-02-28
Published Online:
2013-04-02

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a new tool for rapid identification of bacterial microorganisms. With the development of matrix-assisted laser desorption/ionization time-of-flight-time-of-flight (MALDI-TOF-TOF) instruments, new capabilities for characterizing bacteria and their protein biomarkers and toxins by top-down proteomic identification is now possible. A number of researchers have demonstrated top-down proteomic identification of nondigested proteins using MALDI-TOF-TOF platforms. The current review briefly discusses the use of MS instrumentation for bacterial identification and characterization as well as recent developments in the use of MALDI-TOF-TOF for top-down proteomic identification of protein biomarkers and toxins from unfractionated bacterial cell lysates. This approach provides great promise as a rapid method for identification and characterization of pathogenic microorganisms and their virulence factors.

Keywords: matrix-assisted laser desorption/ionization time-of-flight-time-of-flight (MALDI-TOF-TOF); post-source decay; top-down proteomic identification

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