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Reviews in Analytical Chemistry

Editor-in-Chief: Schechter, Israel

Editorial Board: Pauw, Edwin / Vries, Mattanjah / Grushka, Eli / Laserna, J. / Licht, Stuart / Lubman, David / Mandler, Daniel / Palleschi, Vincenzo / Sigman, Michael / Whitesides, George


IMPACT FACTOR 2017: 2.111

CiteScore 2017: 1.67

SCImago Journal Rank (SJR) 2017: 0.505
Source Normalized Impact per Paper (SNIP) 2017: 0.590

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2191-0189
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Volume 36, Issue 1

Issues

Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs

Martin Bartosik
  • Corresponding author
  • Regional Centre for Applied Molecular Oncology (RECAMO), Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech Republic
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Roman Hrstka
  • Regional Centre for Applied Molecular Oncology (RECAMO), Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-12-21 | DOI: https://doi.org/10.1515/revac-2016-0022

Abstract

Dysregulation of gene expression mechanisms has been observed in many tumors, making their analysis of utmost importance. These mechanisms include DNA methylation, an epigenetic mechanism in which 5-carbon of cytosine becomes methylated, leading to gene silencing, and action of short RNA molecules called microRNAs, which regulate protein synthesis at post-transcriptional level by binding to mRNAs. In this review, we describe major roles of both mechanisms in carcinogenesis, offer an overview of currently used methods for their analysis, and summarize most recent advances in electrochemical-based assays and strategies. Advantages of electrochemistry, including favorable cost, time of experiment, or simple instrumentation, are highlighted, along with current challenges that need to be addressed prior to successful application into clinical routine.

Keywords: cancer diagnostics; DNA methylation; electrochemistry; microRNA; molecular oncology

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About the article

Martin Bartosik

Martin Bartosik received his PhD in Biophysics at Masaryk University in Brno, Czech Republic, in 2012. He worked at the Institute of Biophysics (Brno), where he studied the behavior of nucleic acids and proteins on electrode surfaces. Currently, he works as a junior scientist at the Masaryk Memorial Cancer Institute (Brno), where he develops electrochemical bioassays for detection of various cancer biomarkers, including microRNAs or DNA methylation. His doctoral and postdoctoral stays include the Department of Nanoengineering at the University of California, San Diego, where he was engaged in the construction of DNA hybridization chips and arrays, and Departamento de Química Analítica at Universidad Complutense de Madrid, where he participated at the project of microRNA detection.

Roman Hrstka

Roman Hrstka received his PhD in Cellular and Molecular Biology at Masaryk University in Brno, Czech Republic, in 2005. Since 2001, he has been working at the Masaryk Memorial Cancer Institute in Brno. His work is focused predominately on the role of anterior gradient proteins in the biology of cancer cells. His doctoral and postdoctoral stays include Ninewells Hospital, University of Dundee, where he participated at the project focused on detection of p53 isoforms and CR UK, University of Edinburgh, where he contributed to the study of alternative transcription of DAPK-1. He also received EMBO fellowship at Institut de Génétique Moleculaire in Paris where he was engaged in the project dealing with regulation of p53 translation.


Received: 2016-06-20

Accepted: 2016-10-20

Published Online: 2016-12-21

Published in Print: 2017-03-01


Citation Information: Reviews in Analytical Chemistry, Volume 36, Issue 1, 20160022, ISSN (Online) 2191-0189, ISSN (Print) 0793-0135, DOI: https://doi.org/10.1515/revac-2016-0022.

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