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Reviews on Environmental Health

Editor-in-Chief: Carpenter, David O. / Sly, Peter

Editorial Board: Brugge, Doug / Edwards, John W. / Field, R.William / Garbisu, Carlos / Hales, Simon / Horowitz, Michal / Lawrence, Roderick / Maibach, H.I. / Shaw, Susan / Tao, Shu / Tchounwou, Paul B.


IMPACT FACTOR 2018: 1.616

CiteScore 2018: 1.69

SCImago Journal Rank (SJR) 2018: 0.508
Source Normalized Impact per Paper (SNIP) 2018: 0.664

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2191-0308
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Volume 31, Issue 1

Issues

Proteomics of Sphingobium indicum B90A for a deeper understanding of hexachlorocyclohexane (HCH) bioremediation

Aparna Nandavaram
  • Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India
  • Other articles by this author:
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/ Annapoorni Lakshman Sagar
  • Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India
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/ Ashok Kumar Madikonda
  • Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India
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/ Dayananda Siddavattam
  • Corresponding author
  • Department of Animal Biology, School of Life Sciences, Room No- S40, University of Hyderabad, Hyderabad, Telangana 500046, India
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Published Online: 2016-03-08 | DOI: https://doi.org/10.1515/reveh-2015-0042

Abstract

Genome wide expression profiling of Sphingobium indicum B90A revealed induction of lin genes, linA and linB, involved in dechlorination of hexachlorocyclohexane (HCH), in the presence of all four isomers of HCH. Supporting proteomics data, the qPCR and promoter assay showed upregulation of linA transcription in the presence of HCH isomers. Analysis of the upstream region of the linA gene revealed the existence of the GntR binding site overlapping the -10 hexamer of the putative promoter motif. As GntR is a known transcription repressor its dissociation from the linA promoter is expected to induce lin genes in the presence of HCH isomers. Comparison of in situ and in-culture proteomics indicated expression lin genes at the dumpsite, an indication for the in situ HCH degradation.

Keywords: biodegradation; bioremediation; hexachlorocyclohexane (HCH); lin genes; proteomics

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About the article

Corresponding author: Dayananda Siddavattam, Department of Animal Biology, School of Life Sciences, Room No- S40, University of Hyderabad, Hyderabad, Telangana 500046, India, E-mail:


Received: 2015-10-13

Accepted: 2015-10-13

Published Online: 2016-03-08

Published in Print: 2016-03-01


Citation Information: Reviews on Environmental Health, Volume 31, Issue 1, Pages 57–61, ISSN (Online) 2191-0308, ISSN (Print) 0048-7554, DOI: https://doi.org/10.1515/reveh-2015-0042.

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