Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Reviews in the Neurosciences

Editor-in-Chief: Huston, Joseph P.

Editorial Board: Topic, Bianca / Adeli, Hojjat / Buzsaki, Gyorgy / Crawley, Jacqueline / Crow, Tim / Gold, Paul / Holsboer, Florian / Korth, Carsten / Li, Jay-Shake / Lubec, Gert / McEwen, Bruce / Pan, Weihong / Pletnikov, Mikhail / Robbins, Trevor / Schnitzler, Alfons / Stevens, Charles / Steward, Oswald / Trojanowski, John


IMPACT FACTOR 2018: 2.157
5-year IMPACT FACTOR: 2.935

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 0.980
Source Normalized Impact per Paper (SNIP) 2017: 0.804

Online
ISSN
2191-0200
See all formats and pricing
More options …
Volume 24, Issue 1

Issues

Dissecting circuit mechanisms by genetic manipulation of specific neural pathways

Shigeki Kato
  • Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kenta Kobayashi
  • Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kazuto Kobayashi
  • Corresponding author
  • Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2012-11-12 | DOI: https://doi.org/10.1515/revneuro-2012-0043

Abstract

The major issue in neuroscience is to delineate the structure and function of the neural circuits that mediate a variety of brain functions. One useful approach to resolve this issue is the genetic manipulation of the activity of specific neural circuits, which enables the study of the behavioral and physiological consequences of the manipulation. A novel type of lentiviral vector showing highly efficient retrograde gene transfer (HiRet) can introduce a transgene into neurons that innervate a certain brain region at the vector injection site and confer a fundamental tool for genetically manipulating specific neural pathways. Here, we describe the strategy for the selective targeting of neural pathways by using this HiRet vector, combined with immunotoxin (IT)-mediated cell targeting, which eliminates particular neuronal types genetically engineered to express a receptor for the recombinant IT. Our strategy provides a powerful technology to investigate the framework underlying brain functions and to develop animal models for the dissection of neurological and neuropsychiatric diseases.

Keywords: immunotoxin-mediated cell targeting; neural circuit; retrograde gene transfer; thalamostriatal pathway

About the article

Shigeki Kato

Shigeki Kato completed a BSc and PhD (Agr.) at the Laboratory of Molecular Biology, Graduate School of Agricultural Science in Tohoku University. Then he took a postdoctoral position in Fukushima Medical University with Professor Kazuto Kobayashi, and is now an Assistant Professor. In 2012, he was awarded a PhD (Med.) at Fukushima Medical University. His principal research interests include how the neural circuit is controlled to conduct a variety of behaviors using genetic manipulation of neural circuit with the retrograde gene transfer vector.

Kenta Kobayashi

Kenta Kobayashi graduated from Department of Agricultural Chemistry in Kyoto University, and received a PhD in bioscience from Nara Institute of Science and Technology. He worked as Assistant Professor in Fukushima Medical University, and is an currently Associate Professor at the National Institute of Physiological Sciences, Okazaki, Japan. He is working to clarify the role of Rho family GTPases in brain function using a newly developed double vector system, combining the retrograde gene transfer vector with adeno-associated viral vector.

Kazuto Kobayashi

Kazuto Kobayashi received a PhD in biochemistry from Nagoya University, Japan, and took a post-doc position in Nagoya University School of Medicine, which was supported by the Japan Society for the Promotion of Science. He worked as an Assistant Professor at the Department of Biochemistry in Nagoya University School of Medicine and at the Division of Neurochemistry at the Fujita Health University School of Medicine. He then moved as an Associate Professor to the Division of Molecular Animal Technology at the Nara Institute of Science and Technology and is currently a Professor of Molecular Genetics at the Fukushima Medical University. He is also a Guest Professor at the National Institute of Physiological Sciences, Okazaki, Japan. His laboratory aims to develop new technologies for genetic manipulation of neural circuits and to elucidate the neural mechanism underlying the acquisition, performance, and recovery of instrumental learning.


Corresponding author: Kazuto Kobayashi, Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan


Received: 2012-08-04

Accepted: 2012-08-05

Published Online: 2012-11-12

Published in Print: 2013-02-01


Citation Information: Reviews in the Neurosciences, Volume 24, Issue 1, Pages 1–8, ISSN (Online) 2191-0200, ISSN (Print) 0334-1763, DOI: https://doi.org/10.1515/revneuro-2012-0043.

Export Citation

©2013 by Walter de Gruyter Berlin Boston.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Imran S. Sheikh, Kathleen M. Keefe, Noelle A. Sterling, Ian P. Junker, Chidubem I. Eneanya, Yingpeng Liu, Xiao-Qing Tang, and George M. Smith
Frontiers in Neural Circuits, 2018, Volume 12
[2]
Kenta Kobayashi, Ken-ichi Inoue, Soshi Tanabe, Shigeki Kato, Masahiko Takada, and Kazuto Kobayashi
Frontiers in Neuroanatomy, 2017, Volume 11
[3]
Miyabi Hirano, Shigeki Kato, Kenta Kobayashi, Tomoaki Okada, Hiroyuki Yaginuma, Kazuto Kobayashi, and Joseph Charles Glorioso
PLoS ONE, 2013, Volume 8, Number 9, Page e75896
[4]
Seong-Jin Son, Mansu Kim, and Hyunjin Park
Scientific Reports, 2016, Volume 6, Number 1
[5]
Soshi Tanabe, Ken-ichi Inoue, Hitomi Tsuge, Shiori Uezono, Kiyomi Nagaya, Maki Fujiwara, Shigeki Kato, Kazuto Kobayashi, and Masahiko Takada
Neuroscience Research, 2017, Volume 120, Page 45
[6]
D. Pignataro, D. Sucunza, A. J. Rico, I. G. Dopeso-Reyes, E. Roda, A. I. Rodríguez-Perez, J. L. Labandeira-Garcia, V. Broccoli, S. Kato, K. Kobayashi, and José L. Lanciego
Journal of Neural Transmission, 2017
[7]
Kenta Kobayashi, Shigeki Kato, and Kazuto Kobayashi
Journal of Neural Transmission, 2017
[8]
Yuta Kohro, Emi Sakaguchi, Ryoichi Tashima, Hidetoshi Tozaki-Saitoh, Hideyuki Okano, Kazuhide Inoue, and Makoto Tsuda
Scientific Reports, 2015, Volume 5, Number 1
[9]
Akiya Watakabe, Masafumi Takaji, Shigeki Kato, Kazuto Kobayashi, Hiroaki Mizukami, Keiya Ozawa, Sonoko Ohsawa, Ryosuke Matsui, Dai Watanabe, and Tetsuo Yamamori
Frontiers in Neural Circuits, 2014, Volume 8

Comments (0)

Please log in or register to comment.
Log in