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DNA and RNA Nanotechnology

formerly RNA Nanotechnolgy

Emerging Science

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Triggering RNAi with multifunctional RNA nanoparticles and their delivery

Bich Ngoc Dao / Mathias Viard / Angelica N. Martins / Wojciech K. Kasprzak / Bruce A. Shapiro / Kirill A. Afonin
Published Online: 2015-07-27 | DOI: https://doi.org/10.1515/rnan-2015-0001


Proteins are considered to be the key players in structure, function, and metabolic regulation of our bodies. The mechanisms used in conventional therapies often rely on inhibition of proteins with small molecules, but another promising method to treat disease is by targeting the corresponding mRNAs. In 1998, Craig Mellow and Andrew Fire discovered dsRNA-mediated gene silencing via RNA interference or RNAi. This discovery introduced almost unlimited possibilities for new gene silencing methods, thus opening new doors to clinical medicine. RNAi is a biological process that inhibits gene expression by targeting the mRNA. RNAi-based therapeutics have several potential advantages (i) a priori ability to target any gene, (ii) relatively simple design process, (iii) sitespecificity, (iv) potency, and (v) a potentially safe and selective knockdown of the targeted cells. However, the problem lies within the formulation and delivery of RNAi therapeutics including rapid excretion, instability in the bloodstream, poor cellular uptake, and inefficient intracellular release. In an attempt to solve these issues, different types of RNAi therapeutic delivery strategies including multifunctional RNA nanoparticles are being developed. In this mini-review, we will briefly describe some of the current approaches.

Keywords : RNA nanotechnology; RNA nanoparticles; RNA/DNA hybrids; RNA interference; siRNA; delivery


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About the article

Received: 2015-04-06

Accepted: 2015-05-06

Published Online: 2015-07-27

Citation Information: DNA and RNA Nanotechnology, Volume 2, Issue 1, Pages 1–12, ISSN (Online) 2353-1770, DOI: https://doi.org/10.1515/rnan-2015-0001.

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©2015 Bich Ngoc Dao, et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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