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Romanian Neurosurgery

The Journal of Romanian Society of Neurosurgery

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Experimental model of arteriovenous malformation in vitro using biological grafts

Aurelia Mihaela Sandu
  • Corresponding author
  • PhD student, University of Medicine and Pharmacy “Carol Davila”, Bucharest / Fourth Department of Neurosurgery, Emergency Clinical Hospital Bagdasar Arseni, Bucharest
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ A. Giovani
  • PhD student, University of Medicine and Pharmacy “Carol Davila”, Bucharest / Fourth Department of Neurosurgery, Emergency Clinical Hospital Bagdasar Arseni, Bucharest
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ M.R. Gorgan
  • Fourth Department of Neurosurgery, Emergency Clinical Hospital Bagdasar Arseni, Bucharest / University of Medicine and Pharmacy “Carol Davila”, Bucharest
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-06-16 | DOI: https://doi.org/10.1515/romneu-2015-0023


Introduction: Brain arteriovenous malformations (AVMs) represent a serious health problem all around the world. Experimental models help to better understand the pathophysiology of these lesions. Experiment: We performed an experimental model of AVM using biological grafts, arteries and veins harvested from chicken wings at the elbow joint. We used 14 vessels and we performed 20 end-to-end anastomoses to create a nidus with a single feeding artery and a single draining vein. The system was irrigated with colored solution. The experiment was done according with law in force regarding experimental research activity. Conclusions: Experimental models allow us to understand the hemodynamics and predict the outcome of brain AVMs in humans. This experimental model is a useful tool in understanding the hemodynamic properties of brain AVMs. It is very useful in vascular anastomosis training

Keywords: arteriovenous malformations; biologic grafts; chicken wings; experimental model


  • 1. Al-Shahi R, Bhattacharya JJ, Currie DG, Papanastassiou V, Ritchie V, Roberts RC, Sellar RJ & Warlow CP. (2003). Prospective, population-based detection of intracranial vascular malformations in adults: the Scottish Intracranial Vascular Malformation Study (SIVMS). Stroke 34, 1163-1169.CrossrefGoogle Scholar

  • 2. Al-Shahi R & Warlow C. (2001). A systematic review of the frequency and prognosis of arteriovenous malformations of the brain in adults. Brain 124, 1900-1926.CrossrefGoogle Scholar

  • 3. ApSimon HT, Reef H, Phadke RV & Popovic EA. (2002). A population-based study of brain arteriovenous malformation: long-term treatment outcomes. Stroke 33, 2794-2800.CrossrefGoogle Scholar

  • 4. Berman MF, Sciacca RR, Pile-Spellman J, Stapf C, Connolly ES Jr, Mohr JP & Young WL. (2000). The epidemiology of brain arteriovenous malformations. Neurosurgery 47, 389-396.CrossrefGoogle Scholar

  • 5. Carvi Y & Nievas MN. (2005). Experimental cerebral arteriovenous fistulas. Neurol Res 27, 857-863.CrossrefGoogle Scholar

  • 6. Chen W, Choi Ej, McDougall CM & Su H. (2014). Brain arteriovenous malformation modeling, pathogenesis, and novel therapeutic targets. Transl Stroke Res 5, 316-329.CrossrefWeb of ScienceGoogle Scholar

  • 7. Choi JH & Mohr JP. (2005). Brain arteriovenous malformations in adults. Lancet Neurol 2005, 299-308.CrossrefGoogle Scholar

  • 8. Giovani A, Sandu A, Neacșu A & Gorgan RM (2014). Surgical treatment and outcome of cerebral cavernomas - a 10 years’ experience. Rom Neurosurg 21, 394-404.Google Scholar

  • 9. Gorgan MR, Bucur N, Neacșu A, Sandu AM, Brehar FM, Prună VM & Giovani A. (2014). Aspecte clinice și microchirurgicale în cavernoamele cerebrale (Clinical aspects and microsurgery in brain cavernomas. The XVII-th National Conference of Stroke (AVC) with international participation, 15-17 October 2014, Brașov, Romania. Abstract was published in Rev Rom Stroke (AVC) 17, 62-64.Google Scholar

  • 10. Gorgan MR, Ciubotaru VG, Tătăranu LG, Tașcu A, Iliescu A, Bucur N, Neacșu A, Brehar FM & Sandu AM. (2014). Our experience in a series of 277 brain AVMs, microsurgical treatment and outcome. The 40th Congress of the Romanian Society of Neurosurgery with International Participation, 25-27 September 2014, Bucharest, Romania. Abstract was published in Rom Neurosurgery 21, 525-526.Google Scholar

  • 11. Greenberg MS. (2010). Vascular malformations. In Greenberg MS (Ed), Handbook of neurosurgery (1098-1142). New York: Thieme Medical Publisher.Google Scholar

  • 12. Gutierrez-Gonzalez R, Perez-Zamarron A & Rodriguez-Boto G. (2014). Normal perfusion pressure breakthrough phenomenon: experimental models. Neurosurg Rev 37, 559-568.CrossrefWeb of ScienceGoogle Scholar

  • 13. Hademenos GJ, Massoud TF & Vinuela F. (1996). A biomathematical model of intracranial arteriovenous malformations based on electrical network analysis: theory and hemodynamics. Neurosurgery 38, 1005-1014.CrossrefGoogle Scholar

  • 14. Hartmann A, Mast H, Mohr JP, Koennecke HC, Osipov A, Pile-Spellman J, Duong DH & Young WL. (1998). Morbidity of intracranial hemorrhage in patients with cerebral arteriovenous malformation. Stroke 29, 931-934. 1998.CrossrefGoogle Scholar

  • 15. Herman JM, Spetzler RF, Bederson JB, Kurbat JM & Zabramaki JM. (1995). Genesis of a dural arteriovenous malformation in a rat model. J Neurosurg 83, 539-545.CrossrefGoogle Scholar

  • 16. Hillman J. (2001). Population-based analysis of arteriovenous malformation treatment. J Neurosurg 95, 633-637.CrossrefGoogle Scholar

  • 17. Inagawa S, Isoda H, Kougo H, Isogais S & Sakahara H. (2003). In-vitro simulation of NBCA embolization for arteriovenous malformation. Interv Neuroradiol 9, 351-358.Google Scholar

  • 18. Kerber CW, Hecht ST & Knox K. (1997). Arteriovenous malformation model for training and research. AJNR Am J Neuroradiol 18, 1229-1232.Google Scholar

  • 19. Kwok JC, Hunag W, Leung WC, Chan SK, Chan KY, Leung KM, Chu AC & Lam AK. (2014). Human placenta as an ex vivo vascular model for neurointerventional research. J Neurointerv Surg 6, 394-399.Web of ScienceGoogle Scholar

  • 20. Laakso A & Hernesniemi J. (2012). Arteriovenous malformations: epidemiology and clinical presentation. Neurosurg Clin N Am 23, 1-6.CrossrefGoogle Scholar

  • 21. Marciano FF, Vishteh AG, Apostolides PJ & Spetzler RF. (2000). Arteriovenous malformations - supratentorial. In Kaye AH, Black P (Eds), Operative neurosurgery (1079-1091). London: Harcourt Publishers Limited.Google Scholar

  • 22. Massoud TF, Ji C, Guglielmi G & Vinuela F. (1996). Endovascular treatment of arteriovenous malformations with selective intranidal occlusion by detachable platinum electrodes: technical feasibility in a swine model. AJNR Am J Neuroradiol 17, 1459-1466.Google Scholar

  • 23. Massoud TF, Ji C, Vinuela F, Guglielmi G, Robert J, Duckwiler GR & Gobin YP. (1994). An experimental arteriovenous malformation model in swine: anatomic basis and construction technique. AJNR Am J Neuroradiol 15, 1537-1545.Google Scholar

  • 24. Massoud TF, Vinters HV, Chao KH, Vinuela F & Jahan R. (2000). Histopathologic characteristics of a chronic arteriovenous malformation in a swine model: preliminary study. AJNR Am J Neuroradiol 21, 1268-1276.Google Scholar

  • 25. Ondra SL, Troupp H, George ED & Scawab K. (1990). The natural history of symptomatic arteriovenous malformations of the brain: a 24-year follow-up assessment. J Neurosurg 73, 387-391.CrossrefGoogle Scholar

  • 26. Qian Z, Climent S, Maynar M, Uson-Garallo J, Lima- Rodriguez MAV, Calles C, Robertson H & Castaneda- Zuniga WR. (1999). A simplified arteriovenous malformation model in sheep: feasibility study. AJNR Am J Neuroradiol 20, 765-770.Google Scholar

  • 27. Sandu A & Gorgan M. (2015). Microsurgery and outcome in 277 brain AVMs, a common cause of hemorrhagic stroke in young people. The 9th World Congress on Controversies in Neurology (CONy), 26-28 March 2015, Budapest, Hungary.Google Scholar

  • 28. Sandu AM. (2015). Electronic national registry of brain arteriovenous malformations - a useful tool in monitoring patients with cerebral vascular pathology. Congress of University of Medicine and Pharmacy Carol Davila, 28-30 May 2015, Bucharest, Romania.Google Scholar

  • 29. Sandu AM, Ciubotaru VG, Tătăranu LG, Tașcu A, Bucur N, Neacșu A & Gorgan MR. (2014). Clinical aspects, management and oucome of brain arteriovenous malformations - result with microsurgery first policy. Rom Neurosurg 21, 369-383.Google Scholar

  • 30. Schumacher M & Schellhammer F. (1999). Experimental pseudo arteriovenous malformation. A model for training and research. Interv Neuroradiol 5, 213-217.Google Scholar

  • 31. Soderman M, Andersson T, Karlsson B, Wallace MC & Edner G. (2003). Management of patients with brain arteriovenous malformations. Eur J Radiol 46, 195-205.CrossrefGoogle Scholar

  • 32. Spagmolo E. (2012). Surgical management of cerebral arteriovenous malformations. In Quinones-Hinojosa A (Ed), Schmidek & Sweet operative neurosurgical techniques: indications, methods, and results (1003-1018). Philadelphia: Elsevier-Saunders.Google Scholar

  • 33. Tu J, Karunanayaka A, Windsor A & Stoodley MA. (2010). Comparison of an animal model of arteriovenous malformation with human arteriovenous malformation. J Clin Neurosci 17, 96-102.CrossrefGoogle Scholar

  • 34. Weber F & Knopf H. (2006). Incidental findings in magnetic resonance imaging of the brains of healthy young men. J Neurol Sci 240, 81-84.CrossrefGoogle Scholar

  • 35. Yasargil MG. (1987). Microneurosurgery. IIIA. AVM of the brain, history, embryology, pathological considerations, hemodynamics, diagnostic studies, microsurgical anatomy. Stuttgart: Georg Thieme Verlag; New York: Thieme Medical Publishers, Inc.Google Scholar

  • 36. Young WL, Gao E, Hedemenos GJ & Massoud TF. (2007). Use of modeling for the study of cerebral arteriovenous malformations. In Stieg PE, Batjer HH, Samson D (Eds): Intracranial arteriovenous malformations (49-71). New York: Informa Health Care. Google Scholar

About the article

Published Online: 2015-06-16

Published in Print: 2015-06-01

Citation Information: Romanian Neurosurgery, ISSN (Online) 2344-4959, ISSN (Print) 1220-8841, DOI: https://doi.org/10.1515/romneu-2015-0023.

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© 2015. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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