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Revista Romana de Medicina de Laborator

Romanian Journal of Laboratory Medicine

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The antioxidant potential of Brassica rapa L. on glutathione peroxidase, superoxide dismutase enzymes and total antioxidant status

Saima Gul / Sagheer Ahmed
  • PAPRSB Institute of Health Sciences, University Brunei Darussalam
  • Department of Pharmacy, Faculty of Biological Sciences, Kohat University of Science and Technology, Pakistan
  • Other articles by this author:
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/ Humaira Gul / Kaneez Fatima Shad / Muhammad Zia-Ul-Haq / Diana Badiu
  • Corresponding author
  • Department of Biochemistry, Faculty of Natural and Agricultural Sciences, Ovidius University of Constanta, 124, Mamaia Blvd, 900527 (Romania), Tel: +40 722776327
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Published Online: 2013-06-21 | DOI: https://doi.org/10.2478/rrlm-2013-0008


Research on antioxidant potential from vegetables is increasingly focused on their effects on human health. However, relatively little work has been done to investigate the antioxidant effect of crude extract and/or different fractions from Brassica rapa L. In the present study, the antioxidant potential of crude extract and its fractions from Brassica rapa L. fruit part was tested for glutathione peroxidase (GPx), superoxide dismutase (SOD) enzymes and total antioxidant status (TAS) in blood samples. Our results reveal the fact that crude extract and each analyzed fraction (i.e. aqueous, ethyl acetate and chloroform) showed a concentration dependent effects on GPx, SOD and TAS in respect with saline solution (0.9% NaCl) used as negative control and vitamin C, as positive control. Therefore, GPx levels showed a highest value in crude extract and chloroform fraction (6981 U/L both at 10 mg/ml), SOD levels showed the same results in aqueous and ethyl acetate fractions (220 U/ml both at 10 mg/ml) and TAS in crude extract and all three fractions (i.e. aqueous, ethyl acetate and chloroform, 1.68 mmol/L at 10 mg/ml for all three fractions) in respect with saline solution (p<0.05). Furthermore, vitamin C showed the highest values on all three analyzed enzymes (8769 U/L for GPx, 223 U/ml for SOD and 1.8 mmol/L for TAS at 100 μg/ml). Our investigations have been proved to be promising in terms of future potential applications of crude extract and its fractions as components in a range of phytochemicals composition and/or different pharmaceutical usage, owing to their antioxidant potential.


Cercetările asupra potenţialului antioxidant din legume prezintă o importanţă crescută a efectelor sale asupra sănătaţii umane. Cu toate acestea, puţine studii au fost realizate pentru a investiga efectul antioxidant al extractului crud şi/sau a diferitelor fracţiuni din Brassica rapa L. În studiul de faţă, potenţialul antioxidant al extractului crud şi a fracţiunilor acestuia din fructul Brassica rapa L. a fost testat pentru enzimele glutation peroxidaza (GPx), superoxid dismutaza (SOD) şi statusul total antioxidant (STA) în probe de sânge. Rezultatele noastre au arătat faptul că atât extractul crud cât şi fiecare fracţiune analizată (apoasă, de acetat de etil şi cloroformică) au arătat efecte dependente de concentraţie asupra GPx, SOD şi STA în comparaţie cu soluţia salină (0,9% NaCl) folosită ca şi control negativ şi vitamina C, ca şi control pozitiv. Prin urmare, nivelurile GPx au prezentat valori crescute în extractul crud şi fracţiunea cloroformică (6981 U/L ambele la 10 mg/ml), nivelurile SOD au prezentat aceleaşi rezultate în fracţiunea apoasă şi cea de acetat de etil (220 U/ml ambele la 10 mg/ml) şi STA în extractul crud şi toate cele trei fracţiuni (apoasă, de acetat de etil şi cloroformică; 1,68 mmol/L la 10 mg/ml pentru toate fracţiunile) în comparaţie cu soluţia salină (p<0,05). Mai mult, vitamina C a prezentat cele mai mari valori asupra celor 3 enzime analizate (8769 U/L pentru PGx, 223U/ml pentru SOD şi 1,8 mmol/L pentru STA la 100 μg/ml). Investigaţiile noastre se dovedesc a fi promiţătoare în ceea ce privesc viitoarele aplicabilităţi ale extractului crud şi fracţiunile acestuia ca şi componenţi în diferite preparate fitochimice şi/sau de uz farmaceutic datorită potenţialului lor antioxidant.

Keywords : Brassica rapa L.; glutathione peroxidase; superoxide dismutase; total antioxidant status; enzymes

Cuvinte cheie : Brassica rapa L.; glutation peroxidaza; superoxid dismutaza; status total antioxidant; enzime

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About the article

All authors contributed equally to this work.

Published Online: 2013-06-21

Published in Print: 2013-06-01

Citation Information: Romanian Review of Laboratory Medicine, Volume 21, Issue 2, Pages 161–169, ISSN (Print) 2284-5623, DOI: https://doi.org/10.2478/rrlm-2013-0008.

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