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Scientia Agriculturae Bohemica

The Journal of Czech University of Life Sciences Prague

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1805-9430
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Effect Of Hydrolyzed Milk On The Adhesion Of Lactobacilli To Intestinal Cells*

T. Volštátová
  • Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Prague, Czech Republic
  • Other articles by this author:
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/ J. Havlík
  • Corresponding author
  • Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Prague, Czech Republic
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/ I. Doskočil
  • Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Prague, Czech Republic
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/ M. Geigerová
  • Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Prague, Czech Republic
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/ V. Rada
  • Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Prague, Czech Republic
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Published Online: 2015-04-04 | DOI: https://doi.org/10.1515/sab-2015-0012

Abstract

Milk is an essential part of the human diet and is undoubtedly a major calcium source in human nutrition, accepted well by most individuals. Knowledge on how the components from dairy products support or reduce the adherence of probiotics to the intestinal epithelium is limited. The purpose of this study was to investigate the effect of acid-hydrolyzed milk on the adhesion ability of two potentially probiotic strains (Lactobacillus plantarum S2, Lactobacillus gasseri R) to in vitro human intestinal epithelial model consisting of Caco-2 and mucus-secreting HT29-MTX co-culture. The adhesion of our tested strains L. gasseri and L. plantarum was 4.74 and 7.16%, respectively, when using inoculum of 2 × 108 CFU ml–1. Addition of acid-hydrolyzed milk to co-culture decreased the adherence by 53.7% for L. gasseri R and by 62.2% for L. plantarum S2. The results of this study evidently indicate the potential importance of the food matrix as a factor influencing probiotic colonization of the gut.

Key words: bacterial adhesion; acid-hydrolyzed milk; Lactobacillus gasseri R; Lactobacillus plantarum S2; cells of the small intestine; Caco-2; HT29-MTX

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About the article

Received: 2014-11-05

Accepted: 2015-01-11

Published Online: 2015-04-04

Published in Print: 2015-03-01


*Supported by the Internal Grant Agency of the Czech University of Life Sciences Prague (CIGA), Project No. 20142013, and by Ministry of Education, Youth and Sports of the Czech Republic, Project No. LD14070.


Citation Information: Scientia Agriculturae Bohemica, ISSN (Online) 1805-9430, ISSN (Print) 1211-3174, DOI: https://doi.org/10.1515/sab-2015-0012.

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