Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Scientia Agriculturae Bohemica

The Journal of Czech University of Life Sciences Prague

4 Issues per year

CiteScore 2016: 0.78

SCImago Journal Rank (SJR) 2016: 0.398
Source Normalized Impact per Paper (SNIP) 2016: 0.688

Open Access
See all formats and pricing
More options …

Salinity Stress Tolerance Of Camelina Investigated In Vitro

H. Khalid / M. Kumari
  • Defence Institute of Bio-Energy Research (DIBER), Goraparao, Haldwani, Nainital, India
  • Directorate General of Life Sciences, DRDO HQ, DRDO Bhawan, New Delhi, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ A. Grover / M. Nasim
Published Online: 2015-12-30 | DOI: https://doi.org/10.1515/sab-2015-0028


The ability of Camelina sativa to withstand salinity stress in vitro by adding NaCl (0, 25, 50, 75, 100, 125, 150, 175, 200mM) in Murashige and Skoog basal medium was studied. Performance of the plants was measured in terms of various growth parameters and physiological and biochemical tests performed on fully grown plants. The germination capacity, cotyledon unfolding and first true leaf emergence was reduced by 30.6, 17.3, and 28.8%, respectively in 200mM salt treatment with respect to control. The plant height, relative water content, and plant water content were decreased by 85.4, 10.8, and 9.8%, respectively, in stressed plants with respect to control. A decrease in chlorophyll a and b and total chlorophyll contents (by 81.3%), as well as of protein content was registered. Electrical conductivity increased by 52.8% in stressed plants over control, as expected. Other stress indicators like guiacol peroxidase activity and malondialdehyde also increased with respect to control. At salt concentrations lower than 200mM, no clear cut retardation effects were seen. Thus, the present study opens up the scope of further assessment of survivability of camelina in salt contaminated soils.

Keywords: growth; pigments; proteins; lipid peroxidation



= ascorbate peroxidase


= chlorophyll stability index


= dry weight


= electrical conductivity


= fresh weight


= germination percentage


= guiacol peroxidase


= hydrogen peroxide


= malondialdehyde


= Murashige and Skoog


= hydroxyl radical


= photosystem II


= relative humidity


= reactive oxygen species


= relative water content


= superoxide dismutase


= thiobarbituric acid


= trichloroacetic acid


= turgid weight


  • Agarwal A, Pant T, Ahmed Z (2010): Camelina sativa: a new crop with bio-fuel potential introduced in India. Current Science, 99, 1195.Google Scholar

  • Aghaleh M, Niknam V, Ebrahimzadeh H, Razavi K (2009): Salt stress effects on growth, pigments, proteins and lipid peroxidation in Salicornia persica and S. euro-paea. Biologia Plantarum, 53, 243–248.CrossrefGoogle Scholar

  • Ahmad P, Prasad MNV (2012): Abiotic stress responses in plants: metabolism, productivity and sustainability. Springer-Verlag, New York.Google Scholar

  • Ahmad S, Khan NI, Iqbal MZ, Hussain A, Hassan M (2002): Salt tolerance of cotton (Gossypium hirsutum L.). Asian Journal of Plant Sciences, 1, 715–719.CrossrefGoogle Scholar

  • Ali Y, Aslam Z, Ashraf MY, Tahir GR (2004): Effect of salinity on chlorophyll concentration, leaf area, yield and yield components of rice genotypes grown under saline environment. International Journal of Engineering Science and Technology, 1, 221–225.Google Scholar

  • Arnon DI (1949): Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24, 1–15.CrossrefGoogle Scholar

  • Ashraf M, Ahmad S (2000): Influence of sodium chloride on ion accumulation, yield components and fibre characteristics in salt-tolerant and salt-sensitive line of cotton (Gossypium hirsutum L.). Field Crops Research, 66, 115–127.CrossrefGoogle Scholar

  • Ashraf M, Harris PJC (2004): Potential biochemical indicators of salinity tolerance in plants. Plant Science, 166, 3–16.Google Scholar

  • Ayers R, Westcot W (1985): Water quality for agriculture. FAO Irrigation and Drainage Paper No. 29, FAO, Rome.Google Scholar

  • Azza M, Fatma AM, EL-Quensi EM, Farahat MM (2007): Responses of ornamental plants and woody trees to salinity. World Journal of Agricultural Sciences, 3(3), 386-395.Google Scholar

  • Barrs HD, Weatherley PE (1962): A re-examination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Sciences, 15, 413–428.Google Scholar

  • Batool A, Ashraf M, Akram NA, Al-Qurainy F (2013): Salt-induced changes in growth, some key physio-biochemical attributes, activities of enzymatic and levels of non-enzymatic antioxidants in cauliflower (Brassica oleracea L.). The Journal of Horticultural Science and Biotechnology, 88, 231–241.Google Scholar

  • Bolarian M, Fernandez F, Cruz V, Cuartero J (1991): Salinity tolerance in four wild tomato species using vegetative yield-salinity response curves. The Journal of American Society of Horticultural Sciences, 116, 286–290.Google Scholar

  • Bradford MM (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248–254.CrossrefGoogle Scholar

  • Brouwer C, Goffeau A, Heibloem M (1985): Irrigation water management: Training manual No. 1 – Introduction to irrigation. FAO Corporate Document Repository, www.fao.org/docrep/. 13/03/2001.

  • Epstein E (1980): Responses of plants to saline environments. In: DW Rains, RC Valentine, A Hollaender, eds: Genetic Engineering of Osmoregulation. Plenum Press, New York, 7-21.Google Scholar

  • Garg G (2010): In vitro screening of Catharanthus Roseus L. cultivars for salt tolerance using physiological parameters. International Journal of Environmental Science and Development, 1, 24–30.Google Scholar

  • Ghoulam C, Foursy A, Fares K (2002): Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environmental and Experimental Botany, 47, 39–50.CrossrefGoogle Scholar

  • Grattan S, Grieve C (1998): Salinity–mineral nutrient relations in horticultural crops. Scientia Horticulturae, 78, 127–157.Google Scholar

  • Haleem A, Mohammed MA (2007): Physiological aspects of mungbean plant (Vigna radiata L. Wilczek) in response to salt stress and gibberellic acid treatment. Research Journal of Agricultural and Biological Sciences, 3, 200–213.Google Scholar

  • Hasanuzzaman M, Nahar K, Fujita M (2013): Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In: Ahmad P, Azooz MM, Prasad MNV (eds): Ecophysiology and responses of plants under salt stress. Springer-Verlag, New York, 25–87.Google Scholar

  • Heath RL, Packer L (1968): Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125, 189–198.Google Scholar

  • Jaleel CA, Manivannan P, Murali PV, Gomathinayagam M, Panneerselvam R (2008): Antioxidant potential and indole alkaloid profile variations with water deficits along different parts of two varieties of Catharanthus roseus. Colloids and Surfaces B: Biointerfaces, 62, 312–318.CrossrefWeb of ScienceGoogle Scholar

  • Jampeetong A, Brix H (2009): Effects of NaCl salinity on growth, morphology, photosynthesis and proline accumulation of Salvinia natans. Aquatic Botany, 91, 181–186.CrossrefWeb of ScienceGoogle Scholar

  • Kaloyereas SA (1958): A new method for determining drought resistance. Plant Physiology, 33, 232–233.CrossrefGoogle Scholar

  • Kar M, Feierabend J (1984): Metabolism of activated oxygen in detached wheat and rye leaves and its relevance to the initiation of senescence. Planta, 160, 385–939.Google Scholar

  • Kausar F, Shahbaz M (2013): Interactive effect of foliar application of nitric oxide (NO) and salinity on wheat (Triticum aestivum L.). Pakistan Journal of Botany, 45, 7–73.Google Scholar

  • Lokhande VH, Srivastava S, Patade VY, Dwivedi S, Tripathi RD, Nikam TD, Suprasanna P (2011): Investigation of arsenic accumulation and tolerance potential of Sesuvium portulacastrum (L.) L. Chemosphere, 82, 529–534.Web of ScienceCrossrefGoogle Scholar

  • Misra N, Saxena P (2009): Effect of salicylic acid on proline metabolism in lentil grown under salinity stress. Plant Science, 177, 181–189.Web of ScienceGoogle Scholar

  • Murashige T, Skoog F (1962): A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum, 15, 473–497.CrossrefGoogle Scholar

  • Qasim M, Ashraf M, Ashraf Y, Ahmad R, Nazli S (2004): Some growth related characteristics in canola (Brassica napus L.) under salinity stress. International Journal of Agriculture and Biology, 4, 665–668.Google Scholar

  • Rafiq M, Mali M, Khatri A, Dahot MU (2008): Callus induction and regeneration in local mungbean (Vigna radiate L. Wilczek) under salt stress. Journal of Biotechnology, 136, 147–169.Web of ScienceGoogle Scholar

  • Sabir F, Sangwan RS, Kumar R, Neelam S (2012): Salt stress-induced responses in growth and metabolism in callus cultures and differentiating in vitro shoots of Indian ginseng (Withania somnifera Dunal). Journal of Plant Growth Regulation, 31, 537–548.CrossrefGoogle Scholar

  • Shahbaz M, Zia B (2011): Does exogenous application of glycine betaine through rooting medium alter rice (Oryza sativa L.) mineral nutrient status under saline conditions? Journal of Applied Botany and Food Quality, 84, 54–60.Google Scholar

  • Shaheen S, Naseer S, Ashraf M, Akram N (2012): Salt stress affects water relations, photosynthesis and oxidative defense mechanisms in Solanum melongena L. Journal of Plant Interaction, 8, 85–96.Google Scholar

  • Sharma P, Sardana V, Banga SS (2013): Salt tolerance of Indian mustard (Brassica juncea) at germination and early seedling growth. Environmental and Experimental Biology, 11, 39–46.Google Scholar

  • Siler B, Misic D, Filipovic B, Popovic Z, Cvetic T, Mijovic A (2007): Effects of salinity on in vitro growth and photosynthesis of common centaury (Centaurium erythraea Rafn.). Archives of Biological Sciences, 59, 129–134.Google Scholar

  • Simaei M, Khavarinejad A, Saadatmand S, Bernard F, Fahimi H (2011): Interactive effects of salycylic acid and nitric oxide on soybean plants under NaCl salinity. Russian Journal of Plant Physiology, 58, 783–790.CrossrefGoogle Scholar

  • Taffouo VD, Wamba OF, Yombi E, Nono GV, Akoe A (2010): Growth, yield, water status and ionic distribution response of three bambara groundnut (Vigna subterranean (L.) Verdc.) landraces grown under saline conditions. International Journal of Botany, 6, 53–58.CrossrefGoogle Scholar

  • Tort N, Turkyilmaz B (2004): A physiological investigation on the mechanisms of salinity tolerance in some barley culture forms. Journal of Forest Science, 27, 1–16.Google Scholar

  • Turan MA, Kalkat V, Taban S (2007): Salinity-induced stomatal resistance, proline, chlorophyll and ion concentrations of bean. International Journal of Agricultural Research, 2, 483–488.CrossrefGoogle Scholar

  • Turkan I, Demiral T (2009): Recent developments in understanding salinity tolerance. Environmental and Experimental Botany, 67, 2–9.Web of ScienceCrossrefGoogle Scholar

  • Uhvits R (1964): Effects of osmotic pressure on water absorption and germination of alfalfa seeds. American Journal of Botany, 33, 278–285.CrossrefGoogle Scholar

  • Zhu JK (2002): Salt and drought stress signal transduction in plants. Annual Review of Plant Biology, 53, 247–273.CrossrefGoogle Scholar

  • Zubr J (1997): Oil-seed crop: Camelina sativa. Industrial Crop Production, 6, 113–119.Google Scholar

About the article

Received: 2015-05-28

Accepted: 2015-10-02

Published Online: 2015-12-30

Published in Print: 2015-12-01

Citation Information: Scientia Agriculturae Bohemica, Volume 46, Issue 4, Pages 137–144, ISSN (Online) 1805-9430, ISSN (Print) 1211-3174, DOI: https://doi.org/10.1515/sab-2015-0028.

Export Citation

© 2015 H. Khalid et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Blanca María Plaza, Fernando Paniagua, Miguel Rafael Ruiz, Silvia Jiménez-Becker, and María Teresa Lao
Scientia Horticulturae, 2017, Volume 215, Page 157

Comments (0)

Please log in or register to comment.
Log in