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Scientia Agriculturae Bohemica

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Genetic Diversity Of Plukenetia Volubilis L. Assessed By ISSR Markers*

M. Ocelák
  • Corresponding author
  • Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Tropical Crops and Agroforestry, Prague, Czech Republic
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/ P. Hlásná Čepková
  • Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Tropical Crops and Agroforestry, Prague, Czech Republic
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/ I. Viehmannová
  • Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Tropical Crops and Agroforestry, Prague, Czech Republic
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/ Z. Dvořáková
  • Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Tropical Crops and Agroforestry, Prague, Czech Republic
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/ D.C. Huansi / B. Lojka
  • Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Tropical Crops and Agroforestry, Prague, Czech Republic
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Published Online: 2015-12-30 | DOI: https://doi.org/10.1515/sab-2015-0029


The diversity and genetic relationships in 173 sacha inchi samples were analyzed using ISSR markers. Thirty ISSR primers were used, only 8 showed variability in tested samples. ISSR fragments ranged from 200 to 2500 bp. The mean number of bands per primer was 12 and the average number of polymorphic bands per primer was 11. The lowest percentages of polymorphic bands (27%), gene diversity (0.103), and Shannon’s information index (0.15) were exhibited by the Santa Lucia population, which was also geographically most distant. This fact may be attributed to a very small size of this group. In contrast, the Dos de Mayo population exhibited the highest percentage of polymorphic bands (78%), and the Santa Cruz population the highest Nei’s gene diversity index (0.238) and Shannon’s information index (0.357). The obtained level of genetic variability was 36% among tested populations and 64% within populations. Although the diversity indices were low, a cluster analysis revealed 8 clusters containing mainly samples belonging to individual populations. Principal coordinate analysis clearly distinguished Chumbaquihui, Pucallpa, Dos de Mayo, and Aguas de Oro populations, the others were intermixed. The obtained results indicated the level of genetic diversity present in this location of Peru, although it is influenced by anthropological aspects and independent on the geographical distances.

Keywords: genetic variability; molecular markers; populations; Sacha inchi


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About the article

Received: 2015-05-27

Accepted: 2015-08-15

Published Online: 2015-12-30

Published in Print: 2015-12-01

* Supported by the Internal Grant Agency of the Czech University of Life Science Prague (CIGA), Project No. 20135004, by the Internal Grant Agency of the Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague (IGA), Project No. 20145020, and by the Foundation Nadání Josefa, Marie a Zdeňky Hlávkových.

Citation Information: Scientia Agriculturae Bohemica, Volume 46, Issue 4, Pages 145–153, ISSN (Online) 1805-9430, ISSN (Print) 1211-3174, DOI: https://doi.org/10.1515/sab-2015-0029.

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© 2015 M. Ocelák et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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