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Scientia Agriculturae Bohemica

The Journal of Czech University of Life Sciences Prague

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1805-9430
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Genetic Diversity Of Plukenetia Volubilis L. Assessed By ISSR Markers*

M. Ocelák
  • Corresponding author
  • Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Tropical Crops and Agroforestry, Prague, Czech Republic
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/ P. Hlásná Čepková
  • Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Tropical Crops and Agroforestry, Prague, Czech Republic
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/ I. Viehmannová
  • Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Tropical Crops and Agroforestry, Prague, Czech Republic
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/ Z. Dvořáková
  • Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Tropical Crops and Agroforestry, Prague, Czech Republic
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/ D.C. Huansi / B. Lojka
  • Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, Department of Tropical Crops and Agroforestry, Prague, Czech Republic
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Published Online: 2015-12-30 | DOI: https://doi.org/10.1515/sab-2015-0029

Abstract

The diversity and genetic relationships in 173 sacha inchi samples were analyzed using ISSR markers. Thirty ISSR primers were used, only 8 showed variability in tested samples. ISSR fragments ranged from 200 to 2500 bp. The mean number of bands per primer was 12 and the average number of polymorphic bands per primer was 11. The lowest percentages of polymorphic bands (27%), gene diversity (0.103), and Shannon’s information index (0.15) were exhibited by the Santa Lucia population, which was also geographically most distant. This fact may be attributed to a very small size of this group. In contrast, the Dos de Mayo population exhibited the highest percentage of polymorphic bands (78%), and the Santa Cruz population the highest Nei’s gene diversity index (0.238) and Shannon’s information index (0.357). The obtained level of genetic variability was 36% among tested populations and 64% within populations. Although the diversity indices were low, a cluster analysis revealed 8 clusters containing mainly samples belonging to individual populations. Principal coordinate analysis clearly distinguished Chumbaquihui, Pucallpa, Dos de Mayo, and Aguas de Oro populations, the others were intermixed. The obtained results indicated the level of genetic diversity present in this location of Peru, although it is influenced by anthropological aspects and independent on the geographical distances.

Keywords: genetic variability; molecular markers; populations; Sacha inchi

REFERENCES

  • Aliyu OM, Awopetu JA (2007): Assessment of genetic diversity in three populations of cashew (Anacardium occidentale L.) using protein-isoenzyme-electrophoretic analysis. Genetic Resources and Crop Evolution, 54, 1489–1497. doi: 10.1007/s10722-006-9138-9.CrossrefWeb of ScienceGoogle Scholar

  • Alvarado CT (2008): Collection, identification, taxonomic determination and analysis of concentration of fatty acids of the sacha inchi ecotypes in the Amazonas region. Peru-biodiverso Project. Lima, Peru. (in Spanish)Google Scholar

  • Arévalo G (1995): Cultivation of sacha inchi (Plukenetia volubilis L.) in Amazon. National Programme of Research in Genetic Resources and Biotechnology – PRONARGEB, Experimental Station El Porvenir, Tarapoto. (in Spanish)Google Scholar

  • Beebee TJC, Rowe G (2008): An introduction to molecular ecology. Oxford University Press, New York.Google Scholar

  • Bekele A, Feyissa T, Tesfaye K (2014): Genetic diversity of anchote (Coccinia abyssinica (Lam.) Cong.) from Ethiopia as revealed by ISSR markers. Genetic Resources and Crop Evolution, 61, 707–719.Web of ScienceCrossrefGoogle Scholar

  • Bondiolli P, Della Bella L, Rettke P (2006): Alpha linolenic acid rich oils: composition of Plukenetia volubilis (Sacha Inchi) oil from Peru. Rivista Italiana delle Sostanze Grasse, 123, 120–123.Google Scholar

  • Bussmann RW, Téllez C, Glenn A (2009): Plukenetia huayllabambana sp. nov. (Euphorbiaceae) from the Upper Amazon of Peru. Nordic Journal of Botany, 27, 313–315. doi: 10.1111/j.1756-1051.2009.00460.x.Web of ScienceCrossrefGoogle Scholar

  • Bussmann RW, Zambrana NP, Téllez C (2013): Plukenetia carolis-vegae (Euphorbiaceae) – a new useful species from Northern Peru. Economic Botany, 67, 387–392. doi: 10.1007/s12231-013-9247-2.Web of ScienceCrossrefGoogle Scholar

  • Corazón-Guivin M, Castro-Ruiz D, Chota-Macuyama W, Rodríguez A, Cachique D, Manco E, Del-Castillo D, Renno JF, García-Dávila C (2008): Genetic characterization of accessions from Sacha Inchi National Germplasm Bank in San Martin region. Folia Amazónia, 18, 23–31. (in Spanish)Google Scholar

  • Dostálek T, Münzbergová Z, Plačková I (2014): High genetic diversity in isolated populations of Thesium ebracteatum at the edge of its distribution range. Conservation Genetics, 15, 75–86. doi: 10.1007/s10592-013-0522-7.CrossrefGoogle Scholar

  • Doyle JJ, Doyle JL (1987): A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15.Google Scholar

  • Fisher M, Husi R, Prati D, Peintinger M, van Kleunen M, Schmid B (2000): RAPD variation among and within small and large populations of the rare clonal plant Ranunculus reptans (Ranunculaceae). American Journal of Botany, 87, 1128–1137. doi: 10.2307/2656649.CrossrefGoogle Scholar

  • Flores D (2010): Data base of Perubiodiverso project. Bibliographic information about history and traditional uses of 3 selected plants. Biocomercioperu Project. Lima, Peru. (in Spanish)Google Scholar

  • Guillen MD, Ruiz A, Cabo N, Chirinos R, Pascual G (2003): Characterization of Sacha Inchi (Plukenetia volubilis L.) oil by FTIR spectroscopy and 1H NMR. Comparison with linseed oil. Journal of the American Oil Chemists’ Society, 80, 755–762. doi: 10.1007/s11746-003-0768-z.CrossrefGoogle Scholar

  • Gupta S, Srivastava M, Mishra GP, Naik PK, Chauhan RS, Tiwari SK, Kumar M, Singh R (2008): Analogy of ISSR and RAPD markers for comparative analysis of genetic diversity among different Jatropha curcas genotypes. African Journal of Biotechnology, 7, 4230–4243.Google Scholar

  • Hadian J, Bigdeloo M, Nazeri V, Khadivi-Khub A (2014): Assessment of genetic and chemical variability in Thymus caramanicus. Molecular Biology Reports, 41, 3201–3210. doi: 10.1007/s11033-014-3180-z.CrossrefWeb of ScienceGoogle Scholar

  • Hamaker BR, Valles C, Gilman R, Hardmeier RM, Clark D, García HH, Gonzales AE, Kohlstad I, Castro M (1992): Amino acid and fatty acid profiles of the Inca peanut (Plukenetia volubilis L.). Cereal Chemistry, 69, 461–463.Google Scholar

  • Hartl DL, Clark AC (eds) (2007): Inbreeding, population subdivision, and migration. In: Principles of population genetics. Sinauer Associates, Inc. Publishers, Sunderland, USA, 257.Google Scholar

  • Heywood VH, Iriondo JM (2003): Plant conservation: old problems, new perspectives. Biological Conservation, 113, 321–335. doi: 10.1016/S0006-3207(03)00121-6.CrossrefGoogle Scholar

  • Nei M (1973): Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America, 70, 3321–3323. doi: 10.1073/pnas.70.12.3321.CrossrefWeb of ScienceGoogle Scholar

  • Nybon H (2004): Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology, 13, 1143–1155.CrossrefGoogle Scholar

  • Rao ES, Kadirvel P, Symonds RC, Geethanjali S, Ebert AW (2012): Using SSR markers to map genetic diversity and population structure of Solanum pimpinellifolium for development of a core collection. Plant Genetic Resources, 10, 38–48. doi: 10.1017/S1479262111000955.CrossrefGoogle Scholar

  • Rodrigues HS, Borem de Oliveira A, Gomes Lopes MT, Cruz CD, Chaves FCM, da Silva Bentes JL (2013): Genetic diversity of Sacha Inchi accessions detected by AFLP molecular markers. Revista de Ciencias Agrárias/Amazonian Journal of Agricultural and Environmental Sciences, 56, 55–60. doi: 10.4322/rca.2013.081. (in Portuguese)CrossrefGoogle Scholar

  • Rodríguez Á, Corazón-Guivin M, Cachique D, Mejía K, Del Castillo D, Renno J-F, García-Dávila C (2010): Morphologic differentiation and ISSR (Inter Simple Sequence Repeats) in genus Plukenetia (Euphorbiaceae) in Peruvian Amazon: a new species proposal. Peruvian Journal of Biology, 17, 325–330. (in Spanish)Google Scholar

  • Sathe SK, Hamaker BR, Sze-Tao KWC, Venkatachalam M (2002): Isolation, purification, and biochemical characterization of a novel water soluble protein from Inca peanut (Plukenetia volubilis L.). Journal of Agricultural and Food Chemistry, 50, 4906–4908. doi: 10.1021/jf020126a.CrossrefGoogle Scholar

  • Shilpha J, Silambarasan T, Pandian SK, Ramesh M (2013): Assessment of genetic diversity in Solanum trilobatum L., an important medicinal plant from South India using RADP and ISSR markers. Genetic Resources and Crop Evolution, 60, 807–818.Web of ScienceCrossrefGoogle Scholar

About the article

Received: 2015-05-27

Accepted: 2015-08-15

Published Online: 2015-12-30

Published in Print: 2015-12-01


* Supported by the Internal Grant Agency of the Czech University of Life Science Prague (CIGA), Project No. 20135004, by the Internal Grant Agency of the Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague (IGA), Project No. 20145020, and by the Foundation Nadání Josefa, Marie a Zdeňky Hlávkových.


Citation Information: Scientia Agriculturae Bohemica, ISSN (Online) 1805-9430, ISSN (Print) 1211-3174, DOI: https://doi.org/10.1515/sab-2015-0029.

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© 2015 M. Ocelák et al., published by De Gruyter Open. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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