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Scientia Agriculturae Bohemica

The Journal of Czech University of Life Sciences Prague

4 Issues per year


CiteScore 2016: 0.78

SCImago Journal Rank (SJR) 2016: 0.398
Source Normalized Impact per Paper (SNIP) 2016: 0.688

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Online
ISSN
1805-9430
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Analysis of European Honeybee (Apis Mellifera) Wings Using ATR-FTIR and Raman Spectroscopy: A Pilot Study

V. Machovič
  • University of Chemistry and Technology Prague, Prague, Czech Republic a
  • Institute of Rock Structure and Mechanics of the Czech Academy of Sciences, Prague, a
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/ L. Lapčák / M. Havelcová / L. Borecká / Mi. Novotná / Ma. Novotná / I. Javůrková / I. Langrová
  • Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Prague, a
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/ Š. Hájková
  • Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Prague, a
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/ A. Brožová
  • Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Prague, a
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/ Ing. Dalibor Titěra
  • Corresponding author
  • Bee Research Institute, Dol, Czech Republic a
  • Czech University of Life Sciences Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Zoology and Fisheries, Kamýcká 129, 165 00 Prague-Suchdol, Prague, a
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Published Online: 2017-04-12 | DOI: https://doi.org/10.1515/sab-2017-0004

Abstract

The infrared (ATR-FTIR) and Raman spectroscopy was used for the structural characterization of honeybee wings. Protein, chitin, and lipid characteristic spectral features were detected using both methods. The protein secondary structure was predominantly composed of the β-sheet molecular conformation with β-turns or coil contributions. The vibration modes of the side-chain aromatic amino acid residues (tyrosine, phenylalanine, tryptophan) occurred in the wing spectra. The results of discriminant analysis showed that the infrared spectroscopy of the wing in combination with a multivariate analysis seemed promising for a resolution of the chemical structure of the wings based on lipid, proteins, and chitin content.

Keywords: vibrational spectroscopy; protein; lipid; chitin

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About the article

Received: 2016-01-07

Accepted: 2016-10-06

Published Online: 2017-04-12

Published in Print: 2017-03-01


Citation Information: Scientia Agriculturae Bohemica, ISSN (Online) 1805-9430, DOI: https://doi.org/10.1515/sab-2017-0004.

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© 2017. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. BY-NC-ND 4.0

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