Jump to ContentJump to Main Navigation
Show Summary Details
Weitere Optionen …

Statistical Applications in Genetics and Molecular Biology

Editor-in-Chief: Sanguinetti, Guido


IMPACT FACTOR 2018: 0.536
5-year IMPACT FACTOR: 0.764

CiteScore 2018: 0.49

SCImago Journal Rank (SJR) 2018: 0.316
Source Normalized Impact per Paper (SNIP) 2018: 0.342

Mathematical Citation Quotient (MCQ) 2018: 0.02

Online
ISSN
1544-6115
Alle Formate und Preise
Weitere Optionen …
Band 3, Heft 1

Hefte

Volume 10 (2011)

Volume 9 (2010)

Volume 6 (2007)

Volume 5 (2006)

Volume 4 (2005)

Volume 2 (2003)

Volume 1 (2002)

Statistical Analysis of Adsorption Models for Oligonucleotide Microarrays

Conrad J Burden / Yvonne E Pittelkow / Susan R Wilson
Online erschienen: 09.12.2004 | DOI: https://doi.org/10.2202/1544-6115.1095

Recent analyses have shown that the relationship between intensity measurements from high density oligonucleotide microarrays and known concentration is non linear. Thus many measurements of so-called gene expression are neither measures of transcript nor mRNA concentration as might be expected.

Intensity as measured in such microarrays is a measurement of fluorescent dye attached to probe-target duplexes formed during hybridization of a sample to the probes on the microarray. We develop several dynamic adsorption models relating fluorescent dye intensity to target RNA concentration, the simplest of which is the equilibrium Langmuir isotherm, or hyperbolic response function. Using data from the Affymerix HG-U95A Latin Square experiment, we evaluate various physical models, including equilibrium and non-equilibrium models, by applying maximum likelihood methods. We show that for these data, equilibrium Langmuir isotherms with probe dependent parameters are appropriate. We describe how probe sequence information may then be used to estimate the parameters of the Langmuir isotherm in order to provide an improved measure of absolute target concentration.

Keywords: Gene expression; microarrays; Langmuir adsorption

Artikelinformationen

Online erschienen: 09.12.2004


Quellenangabe: Statistical Applications in Genetics and Molecular Biology, Band 3, Heft 1, Seiten 1–27, ISSN (Online) 1544-6115, DOI: https://doi.org/10.2202/1544-6115.1095.

Zitat exportieren

©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston.Get Permission

Zitierende Artikel

Hier finden Sie eine Übersicht über alle Crossref-gelisteten Publikationen, in denen dieser Artikel zitiert wird. Um automatisch über neue Zitierungen dieses Artikels informiert zu werden, aktivieren Sie einfach oben auf dieser Seite den „E-Mail-Alert: Neu zitiert“.

[1]
Brian Cannon, Antonio R. Campos, Zachary Lewitz, Katherine A. Willets, and Rick Russell
Analytical Biochemistry, 2012, Jahrgang 431, Nummer 1, Seite 40
[2]
Thomas Naiser, Jona Kayser, Timo Mai, Wolfgang Michel, and Albrecht Ott
BMC Bioinformatics, 2008, Jahrgang 9, Nummer 1
[3]
Hans Binder and Stephan Preibisch
Algorithms for Molecular Biology, 2008, Jahrgang 3, Nummer 1
[4]
Y. V. Stirmanov, O. V. Matveeva, and Y. D. Nechipurenko
Journal of Biomolecular Structure and Dynamics, 2018, Seite 1
[5]
Lindsay A. Brown, Steve E. Kalloger, Melinda A. Miller, Ie-Ming Shih, Steven E. McKinney, Jennifer L. Santos, Ken Swenerton, Paul T. Spellman, Joe Gray, C. Blake Gilks, and David G. Huntsman
Genes, Chromosomes and Cancer, 2008, Jahrgang 47, Nummer 6, Seite 481
[6]
Dmitriy Skvortsov, Diana Abdueva, Christina Curtis, Betty Schaub, and Simon Tavaré
Nucleic Acids Research, 2007, Jahrgang 35, Nummer 12, Seite 4154
[7]
Diana Abdueva, Dmitriy Skvortsov, and Simon Tavaré
Nucleic Acids Research, 2006, Jahrgang 34, Nummer 15, Seite e105
[8]
Raad Z. Gharaibeh, Anthony A. Fodor, Cynthia J. Gibas, and Arkady B. Khodursky
PLoS ONE, 2010, Jahrgang 5, Nummer 12, Seite e14464
[9]
Diana Abdueva, Michele R. Wing, Betty Schaub, Timothy J. Triche, and Rodolfo Aramayo
PLoS ONE, 2007, Jahrgang 2, Nummer 9, Seite e913
[10]
Raad Z. Gharaibeh, Joshua M. Newton, Jennifer W. Weller, Cynthia J. Gibas, and Arkady B. Khodursky
PLoS ONE, 2010, Jahrgang 5, Nummer 6, Seite e11048
[11]
Crystal Jaing, Shea Gardner, Kevin McLoughlin, Nisha Mulakken, Michelle Alegria-Hartman, Phillip Banda, Peter Williams, Pauline Gu, Mark Wagner, Chitra Manohar, Tom Slezak, and Ching-Hong Yang
PLoS ONE, 2008, Jahrgang 3, Nummer 5, Seite e2163
[12]
Hans Binder, Mario Fasold, Torsten Glomb, and Cameron Neylon
PLoS ONE, 2009, Jahrgang 4, Nummer 11, Seite e7862
[13]
Conrad J Burden and Hans Binder
Physical Biology, 2009, Jahrgang 7, Nummer 1, Seite 016004
[14]
Daniel R. Noguera, Erik S. Wright, Pamela Camejo, and L. Safak Yilmaz
Applied Microbiology and Biotechnology, 2014, Jahrgang 98, Nummer 23, Seite 9595
[15]
Maral Saadati and Axel Benner
Statistics in Medicine, 2014, Jahrgang 33, Nummer 30, Seite 5347
[16]
Conrad J Burden, Yvonne Pittelkow, and Susan R Wilson
Journal of Physics: Condensed Matter, 2006, Jahrgang 18, Nummer 23, Seite 5545
[17]
Hans Binder
Journal of Physics: Condensed Matter, 2006, Jahrgang 18, Nummer 18, Seite S491

Kommentare (0)