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Scandinavian Journal of Pain

Official Journal of the Scandinavian Association for the Study of Pain

Editor-in-Chief: Breivik, Harald


CiteScore 2018: 0.85

SCImago Journal Rank (SJR) 2018: 0.494
Source Normalized Impact per Paper (SNIP) 2018: 0.427

Online
ISSN
1877-8879
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Volume 16, Issue 1

Issues

Preoperative downregulation of long-noncoding RNA Meg3 in serum of patients with chronic postoperative pain after total knee replacement

R. Giordano
  • Center for Neuroplasticity and Pain (CNAP), SMI, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark
  • Center for Sensory-Motor Interaction (SMI), Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ K.K. Petersen
  • Center for Sensory-Motor Interaction (SMI), Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ M. Santoro / C. Pazzaglia / M. Valeriani / L. Arendt-Nielsen
  • Center for Sensory-Motor Interaction (SMI), Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2017-07-01 | DOI: https://doi.org/10.1016/j.sjpain.2017.04.025

Abstract

Aims

The incidence of chronic pain after total knee replacement (TKR) is approx. 20%, why preoperative risk factors for the development of chronic postoperative pain are highly warranted. Studies have indicated that preoperative inflammatory markers hold prognostic information for the development of chronic postoperative pain. Long-non-coding-RNA (lncRNA) regulates the expression of genes related with e.g. inflammation. The current study aimed to investigate the preoperative influence of lncRNA on the development of chronic postoperative pain following TKR.

Methods

24 patients, who developed chronic postoperative pain and 12 patients with painfree recovery, were sampled from a larger clinical study. Preoperative serum samples were obtained from all patients and analyzed for potential lncRNA candidates. Briefly, total RNA was extracted from serum using miRNeasy Mini kit.cDNA samples were pre-amplified with RT2lncRNAPreAMP Primer Mix that contained specific set of primers to target genes of Human RT2lncRNA Inflammatory Response & Autoimmunity PCR Array. Further, the reaction (25μl) will be aliquoted into the wells of RT2lncRNA PCR Array Human Inflammatory Response & Autoimmunity. LncRNA-expressions were compared between the two groups using student’s t-test.

Results

19 patients were excluded due to the “cut-off” of the statistical analysis’s software that not included the samples because of genomic contamination, retro transcription and amplification’s low efficiency. A total of 13 patients were included (7 with pain, 6 without pain). The preliminary analysis found that the MEG3-lncRNA (implicated in tumor vascularization suppressing) were downregulated in patients who developed chronic postoperative pain compared to patients with a normal recovery (fold change: − 9.56, P < 0.05).

Conclusions

Preoperative MEG-3 is downregulated in patients in risk of developing chronic postoperative pain following TKR. Future research, in larger cohorts should further investigate the role of MEG3 and hence the improvement of the cartilage vascularization and other lncRNAs as predictive indicators for the development of chronic postoperative pain.

About the article

Published Online: 2017-07-01

Published in Print: 2017-07-01


Citation Information: Scandinavian Journal of Pain, Volume 16, Issue 1, Pages 172–173, ISSN (Online) 1877-8879, ISSN (Print) 1877-8860, DOI: https://doi.org/10.1016/j.sjpain.2017.04.025.

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