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Turkish Journal of Biochemistry

Türk Biyokimya Dergisi

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1303-829X
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Association between leptin G-2548A gene polymorphism, plasma leptin levels and lipid profiles in Turkish Cypriot obese subjects / Kıbrıslı Türk obez kişilerde leptin G-2548A gen polimorfizmi ile plazma leptin seviyeleri ve lipid profili arasındaki ilişki

Eda Becer
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  • Near East University, Department of Biochemistry, Faculty of Pharmacy, Nicosia, Turkish Republic of Northern Cyprus
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/ Meral Kızılkanat
  • Near East University, Faculty of Medicine, Department of Medical Genetics, Nicosia, Turkish Republic of Northern Cyprus
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/ Mehtap Tınazlı
  • Near East University, Faculty of Medicine, Department of Internal Medicine, Nicosia, Turkish Republic of Northern Cyprus
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/ Nedime Serakıncı
  • Near East University, Faculty of Medicine, Department of Medical Genetics, Nicosia, Turkish Republic of Northern Cyprus
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Published Online: 2016-01-30 | DOI: https://doi.org/10.1515/tjb-2016-0001

Abstract

Objective: Leptin (LEP) is a metabolic and neuroendocrine hormone which is present in the circulation in amounts proportional to fat mass that acts to reduce food intake and increase energy expenditure thereby regulating body weight homeostasis. Various polymorphisms are shown to be present in LEP gene which play important roles in obesity and obesity-related metabolic biomarkers. The aim of this study was to investigate the association of one of these polymorphisms, leptin gene G-2548A polymorphism, on obesity in association with body mass index (BMI), lipid parameters, plasma leptin levels and homeostasis model assessment of insulin resistance (HOMA-IR).

Methods: The study included 110 obese and 90 non-obese subjects. The LEP G-2548A polymorphism was determined by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). Plasma leptin levels, serum lipid and antropometric parameters were measured.

Results: No association was found between LEP gene G-2548A polymorphism and BMI in both study and control groups. Strikingly study group with obese subjects and with the AA genotype had significantly higher serum total cholesterol (p<0.05) than GA and GG genotypes. In obese group, subjects with the AA genotypes had significantly higher leptin (p<0.05) levels than the GG and GA genotypes.

Conclusion: Our results suggest that the LEP gene G-2548A polymorphism may not be considered as a genetic risk factor for obesity in Turkish Cypriot population. However, the G-2548A polymorphism appear to be important in regulating leptin and total cholesterol levels in obese group through leptin gene expression and signaling.

Özet

Amac: Leptin (LEP), dolaşımdaki miktarı vucuttaki yağ kutlesi ile orantılı olan metabolik ve noroendokrin bir hormondur. Leptin gıda alımını azaltarak ve enerji harcanmasını artırarak vucut ağırlığı homeostazını duzenlemektedir. LEP geninde bulunan bircok polimorfizmin obezite ve obezite ile ilişkili metabolik biyobelirteclerde onemli rol oynadığı gosterilmiştir. Bu calışmanın amacı leptin gen G-2548A polimorfizmi ile obezite, vucut kitle indeksi (VKİ), lipit parametreleri, plazma leptin seviyesi ve homeostatik model değerlendirme indeksi (HOMA-IR) arasındaki ilişkinin belirlenmesidir.

Metod: Calışma 110 obez ve 90 obez olmayan birey icermektedir. LEP G-2548A polimorfizmi belirlenmesi polimeraz zincir reaksiyonu- restriksiyon parca uzunluğu polimorfizmi (PCR-RFLP) ile gercekleştirilmiştir. Calışma kapsamında plazma leptin ve serum lipit seviyeleri, antropometrik parametrelerin olcumu yapılmıştır.

Bulgular: Calışma ve kontrol gruplarında LEP gen G-2548A polimorfizmi ile VKİ arasında ilişki saptanmamıştır. Calışma grubunda AA genotipine sahip obez kişilerin total kolesterol seviyesinin GA ve GG genotipine sahip kişilere gore istatistiksel olarak anlamlı derecede yuksek olduğu gozlemlenmiştir (p<0.05). Ayrıca AA genotipine sahip obez kişilerin leptin seviyelerinin GA ve GG genotiplerine gore daha yuksek olduğu belirlenmiştir (p<0.05).

Sonuc: Sonuclarımız LEP G-2548A gen polimorfizminin Kıbrıslı Turk populasyonunda obezite icin genetik bir risk faktoru olmadığını gostermektedir. Fakat obez kişilerde G-2548A polimorfizminin, leptin gen ekspresyonu ve sinyali uzerine olan etkisi ile bağlantılı olarak leptin ve total kolesterol seviyelerinin duzenlenmesinde onemli bir etkisinin olduğunu duşundurmektedir.

Keywords: Leptin; body mass index; polymorphism; obesity

Anahtar Kelimeler: Leptin; vucut kitle indeksi; polimorfizmi; obezite

About the article

Received: 2015-07-06

Accepted: 2015-12-15

Published Online: 2016-01-30

Published in Print: 2016-02-01


Citation Information: Turkish Journal of Biochemistry, ISSN (Online) 1303-829X, ISSN (Print) 0250-4685, DOI: https://doi.org/10.1515/tjb-2016-0001.

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