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Turkish Journal of Biochemistry

Türk Biyokimya Dergisi


IMPACT FACTOR 2018: 0.329

CiteScore 2018: 0.28

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Source Normalized Impact per Paper (SNIP) 2018: 0.169

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1303-829X
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Volume 41, Issue 6

Issues

Antioxidant potential of some natural and semi-synthetic flavonoid derivatives and the extracts from Maclura pomifera (Rafin.) Schneider (osage orange) and its essential oil composition

Maclura pomifera’dan (Rafin.) Schneider elde edilen ekstre ve bazı flavonoit türevlerinin antioksidan aktivitesi ve uçucu yağ bileşimi

Ilkay Erdogan Orhan
  • Corresponding author
  • Faculty of Pharmacy, Gazi University – Department of Pharmacognosy, Ankara, Turkey, Tel.: +90 312 202 31 86
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Fatma Sezer Senol / Betül Demirci / Margita Dvorska
  • Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 61242 Brno, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Karel Smejkal
  • Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 61242 Brno, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Milan Zemlicka
  • Department of Pharmacognosy and Botany, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-09-23 | DOI: https://doi.org/10.1515/tjb-2016-0125

Abstract

Objective:

The antioxidant potential of various extracts were obtained from the leaf and fruit of Maclura pomifera (Rafin.) Schneider (Moraceae) along with its major isoflavonoids; osajin and pomiferin, their semi-synthetic derivatives; iso-osajin and iso-pomiferin and macluraxanthone, which were assayed.

Methods:

The extracts and compounds were subjected to six experimental models including 2,2′-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD+), and nitric oxide (NO) radical scavenging activity, metal-chelating capacity, ferric- (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) assays by using ELISA methods.

Results:

The fruit aqueous extract exerted higher scavenging activity against DMPD and NO radicals, while the fruit ethyl acetate extract was the most active against DPPH radical (68.61±2.53%). Among the tested compounds, the highest DPPH (91.74±0.26%) and DMPD (30.63±1.31%) radical scavenging effect was observed with macluraxanthone, while pomiferin and iso-pomiferin exhibited better activity than osajin and iso-osajin in all assays except the metal-chelation capacity assay. Phytol was the major compound in both the leaf oils, while the fruit essential oil contained β-caryophyllene as the main component (69.3%).

Discussion and Conclusion:

The current study covers particularly antioxidant capacity of iso-osajin, iso-pomiferin, and macluraxanthone by the aforementioned methods and, among them; pomiferin seems to be a natural possible antioxidant agent.

Özet

Amaç:

Mevcut çalışmada, Maclura pomifera’nın (Rafin.) Schneider (Moraceae) yaprak ve meyvalarından elde edilen çeşitli ekstreler ile başlıca flavonoitleri olan osajin ve pomiferin, bunların yarı-sentetik türevleri olan izo-osajin ve izo-pomiferin ile makluraksanton’un antioksidan potansiyeli incelenmiştir.

Metot:

Ekstreler ve bileşiklere, 2,2′-difenil-1-pikrilhidrazil (DPPH), N,N-dimetil-p-fenilendiamin (DMPD+) ve nitrik oksit (NO) radikal süpürücü aktivite, metal-şelasyon kapasitesi, demir- (FRAP) ve fosfomolibdenyum-indirgeme antioksidan gücü (PRAP) dahil altı metot ELISA mikrotiter yöntemi ile uygulanmıştır.

Bulgular:

Meyva etil asetat ekstresi DPPH radikaline karşı en aktif ekstre iken (%68.61±2.53), meyvanın sulu ekstresi DMPD ve NO radikallerine karşı daha yüksek süpürücü etki göstermiştir. Test edilen bileşikler içinde, metal-şelasyon haricindeki tüm testlerde, pomiferin ve izo-pomiferin, osajin ve izo-osajin’den daha iyi aktivite gösterirken, en yüksek DPPH (%91.74±0.26) ve DMPD (%30.63±1.31 %) radikal süpürücü etki makluraksanton’da görülmüştür. Erkek ve dişi ağaçların yaprakları ile meyvanın uçucu yağı GC-MS ile analiz edilmiştir. Meyva uçucu yağı β-karyofillen’i (%69.3) ana bileşen olarak taşırken, her iki yaprakta da fitol ana bileşendir.

Tartışma ve Sonuç:

Bu çalışma, bahsigeçen yöntemler kullanılarak özellikle izo-osajin, izo-pomiferin ve makluraksanton’un antioksidan kapasitesini kapsamaktadır ve pomiferin doğal muhtemel bir antioksidan ajan olarak görünmektedir.

Keywords: Maclura pomifera; Antioxidant; Osajin; Pomiferin; Macluraxanthone; Essential oil

Anahtar Kelimeler:: Maclura pomifera; Antioksidan; Osajin; Pomiferin; Makluraksanton; Uçucu yağ

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About the article

Received: 2015-04-29

Accepted: 2016-03-29

Published Online: 2016-09-23

Published in Print: 2016-12-01


Conflict of interest statement: The authors have no conflict of interest.


Citation Information: Turkish Journal of Biochemistry, Volume 41, Issue 6, Pages 403–411, ISSN (Online) 1303-829X, ISSN (Print) 0250-4685, DOI: https://doi.org/10.1515/tjb-2016-0125.

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