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Turkish Journal of Biochemistry

Türk Biyokimya Dergisi


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1303-829X
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Volume 41, Issue 6

Issues

Expression of antimicrobial peptides in recurrent adenotonsillitis

Rekürren adenotonsillitte antimikrobiyel peptidlerin ekspresyonu

Mehmet Gökhan Demir / Sedat Aydın
  • Corresponding author
  • Kartal Dr. Lutfi Kirdar Education and Research Hospital, Department of Otolaryngology, İstanbul, Turkey
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Banu Atalay Erdoğan
  • Kartal Dr. Lutfi Kirdar Education and Research Hospital, Department of Otolaryngology, İstanbul, Turkey
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Serpil Oğuztüzün / Murat Kılıç / Nagehan Özdemir Barışık
  • Kartal Dr. Lutfi Kirdar Education and Research Hospital, Department of Pahology, İstanbul, Turkey
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-10-20 | DOI: https://doi.org/10.1515/tjb-2016-0145

Abstract

Background:

Recurrent acute tonsillitis is one of the most frequent otorhinolaryngology clinic referrals, yet its pathogenesis remains poorly understood. Antimicrobial cationic peptides are components of the innate system. They are generally small, highly positively charged peptides with broad spectrum antimicrobial activity which function as the body’s “natural antibiotics”. Our aim is to investigate the role of antimicrobial cationic peptides in the susceptibility of patients to recurrent acute tonsillitis.

Materials and methods:

The study is done with 100 children who had a history of recurrent adenotonsillitis as subject group and 100 children with adenotonsillar hypertrophy as control group. Tonsillar and adenoid tissues are dissected into parts as deep and surface epithelium and investigated semiquantitatively with immunohistochemistry. Human beta defensin (hBD) 1–3 and cathelecidin (LL-37) levels are compared with microscopically.

Results:

Immunohistochemistry revealed a strong expression of hBD-1, hBD-2 and hBD-3 in tonsillar tissue. Quantification of hBD-1, hBD-2 and hBD-3 expressions are shown more in tonsillar tissue than in adenoids. LL-37 is one of the antimicrobial peptides found in human tonsillar tissue and adenoids, that participates in the innate immune system of these tissues. Statistically, hBD-1, hBD-3 and LL-37 expressions were different in recurrent tonsillitis tissue than control (p<0.05). Moreover hBD-2 expression was different in adenoid tissue than control (p<0.05).

Conclusion:

Antimicrobial peptides have key role in adenotonsillar infections and this defense mechanism increases susceptibility to recurrent infections in upper respiratory tract.

Özet

Giriş:

Rekürren akut tonsillitis, kulak burun boğaz kliniğinde sıklıkla görülmektedir fakat patogenezi çok az bilinmektedir. Antimikrobiyal katyonik peptidler doğuştan olan bağışıklık sisteminin parçasıdır. Bu peptidler genel olarak küçük, pozitif yüklü peptidlerdir. Ayrıca vücudumuzun doğal antibiyotiği olarak görev yapan geniş spektrumlu antimikrobiyal aktivite göstermektedirler.Amacımız hastaların tekrarlanan akut tonsillitise duyarlılıklarında antimikrobiyal katyonik peptidlerin rolünü incelemektir.

Metod:

çalışma grubunu rekürren adenotosillit öyküsü olan 100 hasta, kontrol grubunu ise adenotonsiller hipertrofisi olan 100 çocuk oluşturmaktadır. tonsil ve adenoid doku yüzeyel ve derin dokulara ayrılacak şekilde diseke edilmiştir ve semikantitatif olarak immunuhistokimyasal olarak incelenmiştir. human beta defensin (hBD) 1–3 ve katlesidin (LL-37) seviyeleri mikroskopik olarak karşılaştırılmıştır.

Bulgular:

İmmunohistokimyasal değerlendirmede tonsil dokusunda yüksek HbD1, HbD2 ve Hbd3 ekspresyonu mevcuttur. tonsil dokusunda adenoid dokusuna göre daha fazla HbD1, HbD2 ve Hbd3 seviyesi mevcuttur. LL-37 insan tonsil ve adenoid dokusunda yeralan ve doğumsal immun sistemin bir parçası antimikrobiyal peptiddir. hBD1, hBD2 ve LL-37 seviyeleri rekürren tonsillit ve kontrol grubunda istatistiki olarak farklıdır (p<0.05). Ayrıca hBd2 ekspresyonu adenoid dokuda kontrol grubuna göre farklıdır (p<0.05).

Sonuç:

Antimikrobiyel peptidler adenotonsiller enfeksiyonlarda anahtar role sahiptir ve bu defans mekanizması üst solunum yolunda rekürren enfeksiyonlarda hassasiyeti artırır.

Keywords: Antimicrobial peptides; Adenotonsillitis; Adenotonsillar hypertrophy; İmmunohistochemistry; Immune system

Anahtar Kelimeler:: Antimikrobiyel peptidler; Adenotonsillit; Adenotonsiller hipertrofi; Immunuhistokimya; Immun sistem

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About the article

Received: 2015-05-25

Accepted: 2016-04-18

Published Online: 2016-10-20

Published in Print: 2016-12-01


Conflict of interest statement: There is no conflict of interest in this paper and the authors have no financial disclosures.


Citation Information: Turkish Journal of Biochemistry, Volume 41, Issue 6, Pages 437–442, ISSN (Online) 1303-829X, ISSN (Print) 0250-4685, DOI: https://doi.org/10.1515/tjb-2016-0145.

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