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Turkish Journal of Biochemistry

Türk Biyokimya Dergisi


IMPACT FACTOR 2018: 0.329

CiteScore 2018: 0.28

SCImago Journal Rank (SJR) 2018: 0.138
Source Normalized Impact per Paper (SNIP) 2018: 0.169

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1303-829X
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Volume 41, Issue 6

Issues

Investigation of ischemia modified albumin and coenzyme Q10 levels in obese children with metabolic syndrome

Metabolik sendromlu obez çocuklarda iskemi modifiye albumin ve koenzim Q10 düzeylerinin incelenmesi

Sabahattin Muhtaroğlu / Selda Özkan Koçak / İhsan Çetin / Didem Barlak Keti / Mustafa Kendirci
Published Online: 2016-10-12 | DOI: https://doi.org/10.1515/tjb-2016-0147

Abstract

Introduction:

The aim of this study was to analyze serum ischemia modified albumin (IMA) and plasma CoQ10 levels and to evaluate their correlation with insulin resistance (homeostatic model assessment, HOMA) and lipid profile in obese children with and without metabolic syndrome (MS).

Methods:

Thirty-one obese with MS, 30 obese without MS and 34 healthy children aged 6–18 years were included in the study. Serum IMA was measured by colorimetric method, plasma CoQ10 levels were measured by HPLC. Serum glucose, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol and insulin were analyzed.

Results:

IMA levels were found to be significantly higher (p<0.001) while the CoQ10 levels were significantly lower (p<0.001) in obese children with and without MS compared to controls. IMA and CoQ10 significantly correlated with each other and metabolic parameters. Furthermore, IMA and CoQ10 levels did not significantly differ between obese children with and without MS, while glucose, insulin levels and HOMA were significantly higher (p<0.001) in obese children with MS than obese without MS and controls.

Conclusions:

Based on the high levels of IMA, low CoQ10 and association with HOMA and lipid profile; we suggest that obese children may have oxidative damage, lipid peroxidation and cardiometabolic risk.

Özet

Amaç:

Bu çalışmanın amacı; metabolik sendromu (MS) olan ve olmayan obez çocuklarda; serum iskemi modifiye albümin (İMA), plazma koenzim Q10 (CoQ10) düzeylerinin ölçülmesi ve bu parametrelerin insülin direnci (homeostatic model assessment, HOMA) ve lipid profiliyle ilişkisinin değerlendirilmesidir.

Yöntem:

Yaşları 6 ile 15 arasında olan 31 MS’li obez, 30 metabolik sendromu olmayan obez ve 34 sağlıklı çocuk çalışmaya dahil edildi. Serum İMA kolorimetrik yöntemle, plazma CoQ10 seviyeleri HPLC ile ölçüldü. Serum glukoz, total kolesterol (TC), trigliserid (TG), yüksek dansiteli lipoprotein kolesterol (HDL-C), düşük dansiteli lipoprotein kolesterol (LDL-C) ve insülin düzeyleri analiz edildi.

Bulgular:

Obez ve MS’li obez çocuklarda, kontrol grubuna göre İMA seviyeleri istatistiksel olarak anlamlı yüksek (p<0.001), CoQ10 seviyeleri istatistiksel olarak anlamlı düşük (p<0.001) bulundu. İMA ve CoQ10 birbirleriyle ve metabolik parametrelerle anlamlı korelasyon gösterdi. Ayrıca glukoz, insülin düzeyleri ve HOMA MS’li obez çocuklarda, MS’li olmayan obez ve kontrol grubuna göre anlamlı yüksek (p<0.001) iken, İMA ve CoQ10 düzeyleri MS’li obez ve MS’li olmayan obez çocuklar arasında farklılık göstermedi.

Sonuç:

Yüksek İMA, düşük CoQ10 düzeylerine, HOMA ve lipid profiliyle ilişkisine dayanarak; MS’li obez çocukların oksidatif hasar, lipid peroksidasyonu ve kardiyometabolik açıdan risk altında olduğunu söyleyebiliriz.

Keywords: Ischemia modified albumin; Coenzyme Q10; Childhood obesity; Metabolic syndrome; Lipid profile

Anahtar kelimeler:: İskemi modifiye albümin; Koenzim Q10; Çocukluk obezitesi; Metabolik sendrom; Lipit profili

References

  • 1.

    Nadeau KJ, Maahs DM, Daniels SR, Eckel RH. Childhood obesity and cardiovascular disease: links and prevention strategies. Nat Rev Cardiol 2011;8:513–25.Google Scholar

  • 2.

    Lakka HM, Laaksonen DE, Lakka TA, Niskanen LK, Kumpusalo E, Tuomilehto J, et al. The metabolic syndrome and total and cardiovascular disease mortality in middle-aged men. J Am Med Assoc 2002;288:2709–16.Google Scholar

  • 3.

    Liese AD, D’Agostino RB Jr, Hamman RF, Kilgo PD, Lawrence JM, Liu LL, et al. The burden of diabetes mellitus among US youth: prevalence estimates from the SEARCH for Diabetes in Youth Study. Pediatrics2006;118:1510–8.Google Scholar

  • 4.

    Beauloye V, Zech F, Tran HP, Clapuyt P, Maes M. Determinants of early atherosclerosis in obese children and adolescents. J Clin Endocrinol Metab2007;92:3025–32.Google Scholar

  • 5.

    Bijari B, Taheri F, Chahkandi T, Kazemi T, Namakin K, Zardast M. The relationship between serum lipids and obesity among elementary school in Birjand: a case control study. J Res Health Sci 2015;15:83–7.Google Scholar

  • 6.

    Bourdon E, Loreau N, Blache D. Glucose and free radicals impair the antioxidant properties of serum albumin. FASEB J 1999;13:233–44.Google Scholar

  • 7.

    Duarte MM, Rocha JB, Moresco RN, Duarte T, Da Cruz IB, Loro VL, et al. Association between ischemia-modified albumin, lipids and inflammation biomarkers in patients with hypercholesterolemia. Clin Biochem 2009;42:666–71.Google Scholar

  • 8.

    Valle Gottlieb MG, da Cruz IB, Duarte MM, Moresco RN, Wiehe M, Schwanke CH, et al. Associations among metabolic syndrome, ischemia, inflammatory, oxidatives, and lipids biomarkers. J Clin Endocrinol Metab 2010;95:586–91.Google Scholar

  • 9.

    Piva SJ, Tatsch E, De Carvalho JA, Bochi GV, Kober H, Duarte T, et al. Assessment of inflammatory and oxidative biomarkers in obesity and their associations with body mass index. Inflammation 2013;36:226–31.Google Scholar

  • 10.

    Rosenfeldt FL, Haas SJ, Krum H, Hadj A, Ng K, Leong JY, et al. Coenzyme Q10 in the treatment of hypertension: a meta-analysis of the clinical trials. J Hum Hypertens 2007;21:297–306.Google Scholar

  • 11.

    Singh U, Devaraj S, Jialal I. Coenzyme Q10 supplementation and heart failure. Nutr Rev 2007;65:286–93.Google Scholar

  • 12.

    Miles MV, Morrison JA, Horn PS, Tang PH, Pesce AJ. Coenzyme Q10 changes are associated with metabolic syndrome. Clin Chim Acta 2004;344:173–9.Google Scholar

  • 13.

    Crane FL, Hatefi Y, Lester RL, Widmer C. Isolation of aquinone from beef heart mitochondria. Biochim Biophys Acta 1957;25:220–1.Google Scholar

  • 14.

    Frei B, Kim MC, Ames BN. Ubiquinol-10 is an effective lipid soluble antioxidant at physiological concentrations. Proc Natl AcadSci USA 1990;87:4879–83.Google Scholar

  • 15.

    Ernster L, Dallner G. Biochemical, physiological and medical aspects of ubiquinone function. Biochim Biophys Acta 1995;1271:195–204.Google Scholar

  • 16.

    Quintero P, Milagro FI, Campión J, Martínez JA. Impact of oxygen availability on body weight management. Med Hypotheses 2010;74:901–7.Google Scholar

  • 17.

    Hermsdorff HH, Zulet MA, Puchau B, Martínez JA. Central adiposity rather than total adiposity measurements are specifically involved in the inflammatory status from healthy young adults. Inflammation 2011;34:161–70.Google Scholar

  • 18.

    Wood IS, de Heredia FP, Wang B, Trayhurn P. Cellular hypoxia and adipose tissue dysfunction in obesity. Proc Nutr Soc 2009;68:370–7.Google Scholar

  • 19.

    Piva SJ, Duarte MM, Da Cruz IB, Coelho AC, Moreira AP, Tonello R, et al. Ischemia-modified albumin as an oxidative stress biomarker in obesity. Clin Biochem 2011;44:345–7.Google Scholar

  • 20.

    Topaloglu N, Yıldırım S, Tekin M, Binnetoglu FK, Cakir DU, Erdem F, et al. Ischemia-modified albumin as an oxidative stress biomarker in obese children. Nobel Med 2015;11:80–4.Google Scholar

  • 21.

    Baysal T, Alp H, Koc N, Atabek ME, Eklioglu BS, Karaarslan S. Serum ischemia-modified albumin level and its association with cardiovascular risk factors in obese children and adolescents. J Pediatr Endocrinol Metab 2012;25:935–44.Google Scholar

  • 22.

    Gvozdjakova A, Kucharska J, Tkacov M, Singh RB, HlavataA. Ratio of lipid parameters to coenzyme Q10 could be used as biomarker of the development of early complications of obesity in children. Bratisl Lek Listy 2012;113:21–5.Google Scholar

  • 23.

    Bundak R, Furman A, Gunoz H, Darendeliler F, Bas F, Neyzi O. Body mass index for Turkish children. Acta Pediatr 2006;95:194–8.Google Scholar

  • 24.

    Zimmet P, Alberti G, Kaufman F, Tajima N, Silink M, Arslanian S, Di Rosa G, et al. IDF consensus Group. The metabolic syndrome in children and adolescents an IDF consensus report. Pediatr Diabetes 2007;8:299–306.Google Scholar

  • 25.

    Bar-Or D, Lau E, Winkler JV. A novel assay for cobalt-albumin binding and its potential as a marker for myocardial ischemia-a preliminary report. J Emerg Med 2000;19:311–5.Google Scholar

  • 26.

    Keskin M, Kurtoglu S, Kendirci M, Atabek ME, Yazici C. Homeostasis model assessment is more reliable than the fasting glucose/insulin ratio and quantitative insulin sensitivity check index for assessing insulin resistance among obese children and adolescents. Pediatrics 2005;115:500–3.Google Scholar

  • 27.

    Vincent HK, Taylor AG. Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans. Int J Obes 2006;30:400–18.Google Scholar

  • 28.

    Marseglia L, Manti S, D’Angelo G, Nicotera A, Parisi E, et al. Oxidative stress in obesity: a critical component in human diseases. Int J Mol Sci 2015;16:378–400.Google Scholar

  • 29.

    Grzebyk E, Piwowar A. Glycoxidative modification of albumin in medical research. Pol MerkurLekarski2013;34:239–42.Google Scholar

  • 30.

    Zurawska-Płaksej E, Grzebyk E, Marciniak D, Szymańska-Chabowska A, Piwowar A. Oxidatively modified forms of albumin in patients with risk factors of metabolic syndrome. J Endocrinol Invest 2014;37:819–27.Google Scholar

  • 31.

    Sohet FM, Neyrinck AM, Pachikian BD, de Backer FC, Bindels LB, Niklowitz P, et al. Coenzyme Q10 supplementation lowers hepatic oxidative stress and inflammation associated with diet-induced obesity in mice. Biochem Pharmacol 2009;78: 1391–400.Google Scholar

  • 32.

    Menke T, Niklowitz P, de Sousa G, Reinehr T, Andler W. Comparison of coenzyme Q10 plasma levels in obese and normal weight children. Clin Chim Acta 2004;349:121–7.Google Scholar

  • 33.

    Hughes K, Lee BL, Feng X, Lee J, Ong CN. Coenzyme Q10 and differences in coronary heart disease risk in Asian Indians and Chinese. Free Radic Biol Med 2002;32:132–8.Google Scholar

  • 34.

    Kavey RE. Combined dyslipidemia in childhood. J Clin Lipidol 2015;9(5 Suppl):41–56.Google Scholar

  • 35.

    Burke V. Obesity in childhood and cardiovascular risk. Clin Exp Pharmacol Physiol 2006;33:831–7.Google Scholar

  • 36.

    Lannuzzi A, Licenziati MR, Acampora C, Salvatore V, Auriemma L, Romano ML, et al. Increased carotid intima-media thickness and stiffness in obese children. Diabetes Care 2004;27:2506–8.Google Scholar

  • 37.

    Kelishadi R, Sharifi M, Khosravi A, Adeli K. Relationship between C-reactive protein andatherosclerotic risk factors and oxidative stress markers among young persons 10–18 years old. Clin Chem2007;53:456–64.Google Scholar

  • 38.

    Codoner-Franch P, Boix-Garcia L, Simo-Jorda R, Del Castillo-Villaescusa C, Maset-Maldonado J, Valls-Bellés V. Is obesity associated with oxidative stress in children? Int J Pediatr Obes 2010;5:56–63.Google Scholar

  • 39.

    Pravst I, Zmitek K, Zmitek J. Coenzyme Q10 contents in foods and fortification strategies. Crit Rev Food Sci Nutr 2010;50:269–80.Google Scholar

About the article

Received: 2014-12-07

Accepted: 2016-04-19

Published Online: 2016-10-12

Published in Print: 2016-12-01


Ethical issues: The subjects were consecutively registered into the study between January 2010 and April 2010. Informed parental consent was attained to be eligible for registration into the study. The study protocol was certified by the Ethics Committee of XXX, Scientific Research Evaluation Ethical Committee (Date: 08.10.2009/Decision No: 2009-73).

Conflict of interest: The authors declare that there is no conflict of interest in this work.


Citation Information: Turkish Journal of Biochemistry, Volume 41, Issue 6, Pages 443–449, ISSN (Online) 1303-829X, ISSN (Print) 0250-4685, DOI: https://doi.org/10.1515/tjb-2016-0147.

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