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Turkish Journal of Biochemistry

Türk Biyokimya Dergisi


IMPACT FACTOR 2018: 0.329

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1303-829X
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Band 41, Heft 6

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Association between MTHFR C677T polymorphism and folate, vitamin B12, homocysteine, and DNA fragmentation in patients with ovarian cancer

Over kanserli hastalarda MTHFR C677T polimorfizmi ile folat, vitamin B12, homosistein düzeyleri ve DNA kırıkları arasındaki ilişki

Anıl Çağla Özkılıç / Ahmet Çetin
  • Haseki Education and Research Hospital, Department of Obstetrics and Gynecology, Istanbul, Turkey
  • Weitere Artikel des Autors:
  • De Gruyter OnlineGoogle Scholar
/ Burcu Bayoğlu
  • Korrespondenzautor
  • Istanbul University, Cerrahpaşa Medical Faculty, Department of Medical Biology, Istanbul, Turkey, Phone: +90 0212 414 30 00/22657, Fax: +90 0212 586 15 48
  • E-Mail
  • Weitere Artikel des Autors:
  • De Gruyter OnlineGoogle Scholar
/ Huriye Balcı
  • Istanbul University, Cerrahpaşa Medical Faculty, Fikret Biyal Central Research Laboratory, Istanbul, Turkey
  • Weitere Artikel des Autors:
  • De Gruyter OnlineGoogle Scholar
/ Müjgan Cengiz
  • Korrespondenzautor
  • Istanbul University, Cerrahpaşa Medical Faculty, Department of Medical Biology, Istanbul, Turkey, Phone: +90 0212 414 30 00/22657, Fax: +90 0212 586 15 48
  • E-Mail
  • Weitere Artikel des Autors:
  • De Gruyter OnlineGoogle Scholar
Online erschienen: 08.10.2016 | DOI: https://doi.org/10.1515/tjb-2016-0151

Abstract

Objective:

Methylenetetrahydrofolate reductase (MTHFR) is an important enzyme that regulates the metabolism of methionine and folate. MTHFR C677T polymorphism was reported to be associated with breast and ovarian cancer. The aim of this study was to evaluate the association between the MTHFR C677T (rs1801133) polymorphism and homocysteine, vitamin B12, and folate levels, and DNA fragmentation in patients with ovarian cancer and healthy controls.

Materials and methods:

This case-control study was conducted in Istanbul University Cerrahpasa Medical Faculty. We studied 50 ovarian cancer patients and 54 healthy controls. The MTHFR C677T polymorphism was determined by PCR followed by restriction fragment length polymorphism (RFLP) and agarose gel electrophoresis. DNA fragmentation was assessed by the comet assay. Homocysteine levels were measured by ELISA, whereas vitamin B12 and folate levels were measured by chemiluminescence methods.

Results:

We found no correlation between the MTHFR C677T polymorphism and ovarian cancer. No significant difference was found in homocysteine, folate, and vitamin B12 levels between patient and control groups. Increased DNA fragmentation was detected in patients with ovarian cancer.

Conclusion:

Our findings suggest that MTHFR C677T polymorphism, as well as homocysteine, folic acid, and vitamin B12 levels, are not associated with an increased risk for ovarian cancer.

Özet

Amaç:

Metilentetrahidrofolat redüktaz (MTHFR), metionin ve folat metabolizmasını düzenleyen önemli bir enzimdir. Spesifik folat metabolitleri; DNA sentezi, tamiri ve metilasyonu için metil grubu vericisi olduğundan, folat metabolizması, DNA sentez ve tamirinde önemli bir role sahiptir ve kanser gelişimini etkileyebilmektedir. MTHFR C677T (rs1801133) polimorfizminin meme ve over kanseri ile ilişkili olduğu bildirilmiştir. Çalışmamızda, over kanserli hastalar ile sağlıklı kontrollerde, MTHFR geni C677T (rs1801133) polimorfizmi ile homosistein, B12 vitamini, folat düzeyleri ve DNA kırık düzeylerinin belirlenerek karşılaştırılması amaçlanmıştır.

Gereç ve Yöntemler:

Çalışmamızda 50 over kanserli hasta ile 54 sağlıklı kontrolde MTHFR C677T (rs1801133) polimorfizmi genotipleri, polimeraz zincir reaksiyonu (PZR) işlemini takiben restriksiyon parça uzunluk polimorfizmi (RFLP) ve agaroz jel elektroforezi yöntemleri ile belirlendi. Over kanserli hastalarda meydana gelen DNA kırıkları Comet deneyi ile gösterildi. Homosistein düzeyleri ELİZA testi, B12 vitamini ve folat düzeyleri ise kemilüminesans yöntemi ile tayin edildi.

Bulgular:

Çalışmamızda MTHFR C677T (rs1801133) polimorfizmi ile over kanseri arasında bir ilişki bulunmadı. Hasta ve kontrol grubunda homosistein, folat ve B12 vitamini düzeyleri karşılaştırıldığında aralarında istatistiksel olarak anlamlı bir fark bulunmadı. Over kanserli hastalarda DNA kırık sayısının hastalık şiddeti ile birlikte arttığı belirlendi.

Sonuçlar:

Sonuç olarak, MTHFR C677T polimorfizmi ile homosistein, folat ve B12 vitamini düzeylerinin hasta ve kontrol grubu arasında farklı bulunmaması, MTHFR C677T polimorfizmi ile homosistein, folat ve B12 vitamini düzeylerinin over kanseri patogenezi ile ilişkili olmadığını düşündürmektedir. Ancak sonuçların anlamlı bulunmaması hasta sayımızın azlığına bağlı olabilir.

Keywords: Ovarian cancer; MTHFR gene; C677T polymorphism; Homocysteine; Folic acid; Vitamin B12; Comet assay

Anahtar kelimeler:: Over kanseri; MTHFR geni; C677T polimorfizmi; Homosistein; Folik asit; B12 vitamini; Comet testi

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Artikelinformationen

Erhalten: 01.07.2015

Angenommen: 19.04.2016

Online erschienen: 08.10.2016

Erschienen im Druck: 01.12.2016


Conflict of interest statement: Authors have no conflict of interest regarding this study.


Quellenangabe: Turkish Journal of Biochemistry, Band 41, Heft 6, Seiten 459–465, ISSN (Online) 1303-829X, ISSN (Print) 0250-4685, DOI: https://doi.org/10.1515/tjb-2016-0151.

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