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Turkish Journal of Biochemistry

Türk Biyokimya Dergisi


IMPACT FACTOR 2018: 0.329

CiteScore 2018: 0.28

SCImago Journal Rank (SJR) 2018: 0.138
Source Normalized Impact per Paper (SNIP) 2018: 0.169

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1303-829X
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Volume 43, Issue 4

Issues

Evaluation of resveratrol organogels prepared by micro-irradiation: fibroblast proliferation through in vitro wound healing

Mikrodalga yöntemi ile hazırlanan resveratrol içeren organojellerin fibroblast proliferasyonuna etkisinin in vitro yara iyileşme modeliyle değerlendirilmesi

Çiğdem Çelen / Ceren Keçeciler / Evren Alğın Yapar / Evren Homan Gökçe
  • Corresponding author
  • Faculty of Pharmacy, Ege University, Bornova-İzmir 35100, Turkey, Phone: 00902323115132, Fax: 00902323115132
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/ Ayşe Nalbantsoy
  • Corresponding author
  • Faculty of Engineering, Department of Bioengineering, Ege University, Bornova-İzmir 35100, Turkey, Phone: 00902323884955, Fax: 00902323884955
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Published Online: 2017-09-09 | DOI: https://doi.org/10.1515/tjb-2016-0283

Abstract

Objective

Resveratrol (RSV) has therapeutic potential with several biological activity. The aim of this study was to develop organogel formulations of RSV and to investigate the proliferation and migration via in vitro wound model of primary normal human skin fibroblasts (NHDF).

Methods

The optimum RSV concentration was determined by MTT assay. Three different types of polyethylene glycol (200, 400 and 600) were used to prepare Carbopol 940 based organogels by micro-irradiation method. Differential scanning microscopy (DSC) and rheological analyses were conducted. Proliferation activity and migration of fibroblast cells were determined with the Giemsa staining.

Results

The percentage of migration rate obtained with RSV-PEG-400 organogel was the highest, as 59.7%. The values obtained with RSV-PEG-200 and RSV-PEG-600 were 36.6% and 48.7%, respectively.

Conclusion

This study showed that RSV organogels produced by micro-irradiation method could be a model for active molecules that have low water solubility for different applications as pharmaceuticals, and cosmeceuticals.

Özet

Amaç

Resveratrol (RSV) pek çok biyolojik aktivite ve terapötik potansiyele sahip bir moleküldür. Çalışmanın amacı RSV içeren organojel formülasyonları geliştirmek ve NHDF (Normal Human Dermal Fibroblast) hücreleri ile oluşturulan in vitro yara modeli kullanılarak proliferasyon ve migrasyon üzerindeki etkinliğini belirlemektir.

Yöntemler

Organojel içeriğindeki en uygun RSV konsantrasyonu MTT testi ile belirlenmiştir. Üç farklı tip polietilen glikol (200, 400 ve 600) ve Carbopol 940 kullanılarak mikrodalga uygulaması ile organojeller elde edilmiştir. Oluşturulan organojel formülasyonlarının DSC ve reolojik analizleri gerçekleştirilmiştir. İn vitro yara modeli üzerinde Giemsa boyama ile RSV içeren organojellerin fibroblast hücrelerinin proliferasyon aktivitesi ve migrasyonu üzerindeki etkinliği belirlenmiştir.

Sonuçlar

En yüksek migrasyon oranı PEG-400 ile oluşturulan RSV içeren organojellerde %59.7 oranında belirlenmiştir. RSV-PEG-200 ve RSV-PEG-600 ise sırasıyla 36.6% ve 48.7% olarak belirlenmiştir.

Tartışma

Bu çalışma mikrodalga yöntemi ile geliştirilen RSV içeren organojel formülasyonları çözünürlüğü düşük aktif moleküllerin farmasötik ve kozmetik gibi farklı uygulama alanlarında kullanımı için model olarak kullanılabilir.

Keywords: Resveratrol; Organogel; Polyethylene glycol; Proliferation; Wound healing; CytoSelect™

Anahtar kelimeler: Resveratrol; Organojel; Polietilen glikol; Proliferasyon; Yara modeli; CytoSelect™

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About the article

Received: 2016-12-20

Accepted: 2017-04-04

Published Online: 2017-09-09


Conflict of interest statement: There are no conflicts of interest among the authors.


Citation Information: Turkish Journal of Biochemistry, Volume 43, Issue 4, Pages 385–392, ISSN (Online) 1303-829X, DOI: https://doi.org/10.1515/tjb-2016-0283.

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