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Turkish Journal of Biochemistry

Türk Biyokimya Dergisi


IMPACT FACTOR 2018: 0.329

CiteScore 2018: 0.28

SCImago Journal Rank (SJR) 2018: 0.138
Source Normalized Impact per Paper (SNIP) 2018: 0.169

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1303-829X
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Volume 43, Issue 4

Issues

Oxidative stress and response of antioxidant system in Nostoc muscorum exposed to different forms of Zinc

Farklı Çinko formlarına maruz bırakılan Nostoc muscorum‘da oksidatif stres ve antioksidan sistemin tepkisi

Sadaf Mahfooz / Sadaf Jahan / Adeeba Shamim / Arbab Husain / Alvina FarooquiORCID iD: http://orcid.org/0000-0002-7260-0414
Published Online: 2018-01-19 | DOI: https://doi.org/10.1515/tjb-2017-0236

Abstract

Objective

Present study aims to evaluate oxidative stress-mediated toxicity mechanisms of nano-ZnO (N-ZnO) compared to ZnCl2 and bulk-ZnO (M-ZnO), using cyanobacterium as a toxicity assessment tool.

Methods

Nostoc muscorum was used as a test organism and the experiments were performed in parallels after treated with 2–128 μM of N-ZnO, M-ZnO, and ZnCl2.

Results

Low concentrations of ZnCl2 (2–8 μM) and N-ZnO (16–32 μM) led to a significant increase in the carotenoids, which could be ascribed to its role as an antioxidant, whereas higher concentrations were inhibitory to the photosynthetic pigments. Enhanced activities of superoxide dismutase (SOD), peroxidase (POD) and accelerated lipid peroxidation depicted significant accumulation of superoxide and peroxide radicals in N. muscorum exposed to N-ZnO and ZnCl2. The non-enzymatic antioxidants such as proline and total phenols also showed enhanced accumulations up to 16 and 64 μM of ZnCl2 and N-ZnO treatment, respectively.

Conclusion

Our results for the very first time depicted the significant accumulation of stress biomarkers in N. muscorum in response to deleterious effects of oxidative stress caused by N-ZnO, ZnCl2, and M-ZnO. Present results revealed varying levels of toxicity of various forms of zinc, which decreased in an order of ZnCl2>N-ZnO>M-ZnO.

Özet

Amaç

Bu çalışmada, toksisite değerlendirme aracı olarak siyanobakteri kullanarak ZnCl2 ve bulk-ZnO (M-ZnO) ile karşılaştırıldığında, nano-ZnO‘in (N-ZnO) oksidatif stres aracılı toksisite mekanizmasını değerlendirmeyi amaçladık.

Yöntemler

Bir test organizması olarak Nostoc muscorum kullanımıştır, deneyler parallel olarak 2–128 μM N-ZnO, M-ZnO ve ZnCl2 ile muamele edildikten sonra gerçekleştirilmiştir.

Bulgular

Düşük konsantrasyonda ZnCl2 (2–8 μM) ve N-ZnO (16–32 μM) antioksidan rolünü destekleyen bir şekilde, karotenoidlerde belirgin bir artış göstermiştir, bunun yanısıra daha yüksek konsantrasyonlar fotosentetik pigmentleri inhibe etmiştir. Süperoksit dismutaz (SOD), peroksidaz (POD) aktivitelerindeki artış ve hızlandırılmış lipit peroksidasyonu, N-ZnO ve ZnCl2‘ye maruz bırakılan N. muscorum‘da süperoksit ve peroksit radikallerinin önemli bir birikimine işaret etmiştir. 16 μM ZnCl2 ve 64 μM N-ZnO uygulamaları sonucunda Prolin ve toplam fenoller gibi enzimatik olmayan antioksidanlarda da yüksek birikimler gösterilmiştir.

Sonuç

Bu çalışmanın sonuçları ilk kez, N-ZnO, ZnCl2 ve M-ZnO‘nun neden olduğu zararlı etkilere yanıt olarak N. muscorum‘da oksidatif stres biyobelirteçlerinin önemli birikiminin olduğunu göstermiştir. Mevcut sonuçlar ZnCl2>N-ZnO>M-ZnO sıralamasında, çeşitli çinko formlarının, düşen toksisitesinin seviyelerini ortaya koymuştur.

Keywords: Cyanobacteria; Photosynthetic pigments; Oxidative stress; SOD; POD; Proline; Phenolic compounds; Zinc oxide nanoparticles; Zinc chloride

Anahtar kelimeler: Siyanobakteriler; Fotosentetik pigmentler; Oksidatif stres; SOD; POD; Prolin; Fenolik bileşikler; çinko oksit nanopartiküller; Çinko Klorid

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About the article

Received: 2017-08-29

Accepted: 2017-10-31

Published Online: 2018-01-19


Conflict of interest statement: The authors declare that they have no conflict of interest regarding this study.


Citation Information: Turkish Journal of Biochemistry, Volume 43, Issue 4, Pages 352–361, ISSN (Online) 1303-829X, DOI: https://doi.org/10.1515/tjb-2017-0236.

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