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Turkish Journal of Biochemistry

Türk Biyokimya Dergisi


IMPACT FACTOR 2018: 0.329

CiteScore 2018: 0.28

SCImago Journal Rank (SJR) 2018: 0.138
Source Normalized Impact per Paper (SNIP) 2018: 0.169

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1303-829X
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Volume 43, Issue 4

Issues

Wendlandia exserta: a pertinent source of antioxidant and antimicrobial agent

Wendlandia exserta: Antioksidan ve antimikrobial maddeler için uygun bir kaynak

Tayyaba ShahzadiORCID iD: http://orcid.org/0000-0002-4395-0490 / Tauheeda Riaz / Muhammad Athar Abbasi / Farhana Mazhar / Muhammad Shahid / Muhammad Ajaib
Published Online: 2018-03-17 | DOI: https://doi.org/10.1515/tjb-2017-0253

Abstract

Objective

The aim of the present investigation was to assess the antioxidant, antibacterial and antifungal activities of various fractions of Wendlandia exserta (Roxbukan Pansara).

Methods

The methanolic extract of W. exserta (Roxbukan Pansara) was dissolved in water (distilled) and then partitioned with solvents e.g. n-hexane, CHCl3, EtOAc and n-BuOH consecutively. Antioxidant potential of above mentioned fractions and remaining aqueous fraction was checked by four methods. Antimicrobial activity was examined by four bacteria and four fungi by the disc diffusion method by using a standard, as positive control.

Results

The aqueous fraction displayed maximum value of diphenyl picryl hydrazyl (DPPH) radical inhibition (85.94%±0.35) with an IC50 value of 415.31±0.26 μg mL−1. The aqueous fraction also displayed the maximum ferric reducing antioxidant power (FRAP) value [64.5±0.15 μg of trolox equivalents (TE)] as well as maximum inhibition of lipid peroxidation (42.32%±0.59) and maximum value of total phenolic contents (87.10±1.10). However, the n-BuOH fraction was found to display the maximum value of total antioxidant activity (0.529±0.76). The fractions also displayed good antimicrobial activity against various microorganisms.

Conclusions

It was concluded that polar fractions of W. exserta showed good antioxidant and antimicrobial activity, so these might be useful in pharmacological preparations.

Özet

Amaç

Bu araştırmanın amacı, Wendlandia exserta (Roxbukan Pansara) ’nın çeşitli fraksiyonlarının antioksidan, antibakteriyel ve antifungal aktivitelerini değerlendirmektir.

Yöntemler

Wendlandia exserta’nın (Roxbukan Pansara) metanolik ekstraktı suda çözülmüş (damıtılmış) ve daha sonra farklı çözücülerde, ör. n-heksan, CHCl3, EtOAc ve n-BuOH sırayla ayrıştırılmıştır. Yukarıda belirtilen fraksiyonların ve kalan sulu fraksiyonun antioksidan etkisi dört yöntemle kontrol edildi. Antimikrobiyal aktivite, standart olarak pozitif kontrol kullanılarak disk difüzyon yöntemi ile dört bakteri ve dört mantar üzerinde incelendi.

Bulgular

Sulu fraksiyon 415,31±0,26 μg mL−1 IC50 değeri ile DPPH radikal inhibisyonunun maksimum değerini (% 85,94±0,35) gösterdi. Sulu fraksiyon ayrıca maksimum lipoproteinasyon inhibisyonu (% 42,32±0,59) ve toplam fenolik içeriğin maksimum değeri (87,10±1,10) yanı sıra maksimum FRAP değeri (64,5±0,15 μg troloks eşdeğerleri) göstermiştir. Bununla birlikte, n-BuOH fraksiyonunun toplam antioksidan etkinliğinin en yüksek (0,529±0.76) olduğu bulunmuştur. Fraksiyonlar ayrıca çeşitli mikroorganizmalara karşı iyi antimikrobiyal etki göstermiştir.

Sonuçlar

Wendlandia exserta’nın polar fraksiyonlarının iyi antioksidan ve antimikrobiyal aktivite gösterdiği sonucuna varılmış, bu nedenle bu fraksiyonların farmakolojik preparatlarda yararlı olabileceği sonucuna varılmıştır.

Keywords: Wendlandia exserta (Roxbukan Pansara); FRAP assay; FTC assay; Total phenolics; Total antioxidant activity; DPPH assay; Antimicrobial activity; Disc diffusion method

Anahtar kelimeler: Wendlandia exserta (Roxbukan Pansara); FRAP testi; FTC testi; Toplam fenolik madde; Toplam antioksidan aktivite; DPPH testi; Antimikrobiyal aktivite; Disk difüzyon yöntemi

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About the article

Received: 2017-09-19

Accepted: 2017-12-26

Published Online: 2018-03-17


Conflict of interest: The authors have no conflict of interest for this manuscript.


Citation Information: Turkish Journal of Biochemistry, Volume 43, Issue 4, Pages 456–463, ISSN (Online) 1303-829X, DOI: https://doi.org/10.1515/tjb-2017-0253.

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