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Turkish Journal of Biochemistry

Türk Biyokimya Dergisi


IMPACT FACTOR 2018: 0.329

CiteScore 2018: 0.28

SCImago Journal Rank (SJR) 2018: 0.138
Source Normalized Impact per Paper (SNIP) 2018: 0.169

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1303-829X
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Volume 44, Issue 1

Issues

Effects of platelet rich plasma on the gastric serosal surface neomucosa formation: an experimental rodent model

Trombositten zengin plazmanın gastrik serozal yüzeydeki neomukoza oluşumuna etkileri: deneysel kemirgen modeli

Binboğa Sinan
  • Corresponding author
  • Department of General Surgery, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, Bakirkoy, Istanbul, Turkey
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/ Kasapoğlu Pınar
  • Department of Biochemistry, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, Bakirkoy, Istanbul, Turkey
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/ Binboğa Elif
  • Department of General Surgery, Bagcilar Training and Research Hospital, Bagcilar, Istanbul, Turkey
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/ Cikot Murat
  • Department of General Surgery, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, Bakirkoy, Istanbul, Turkey
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/ Baytekin Fırat
  • Department of Pathology, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, Bakirkoy, Istanbul, Turkey
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/ Yaprak Saraç Elif
  • Department of Medical Services and Techniques, Istanbul Bilgi University, Şişli, Istanbul, Turkey
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/ Bicer Gencbay Mualla
  • Department of Physical Medicine and Rehabilitation, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, Bakirkoy, Istanbul, Turkey
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/ Halil Alis
  • Department of General Surgery, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, Bakirkoy, Istanbul, Turkey
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/ Işıksaçan NilgünORCID iD: https://orcid.org/0000-0002-0230-6500
Published Online: 2018-09-27 | DOI: https://doi.org/10.1515/tjb-2018-0098

Abstract

Background

Autologous platelet rich plasma (PRP) is the platelet concentration obtained from thrombocytes in the plasma. During the healing process, the platelets are activated and then release the granules which stimulate the inflammatory cascade and healing process. Platelet derived growth factor, vascular endothelial growth factor (VEGF), transforming growth factor β (TGFβ), epidermal growth factor (EGF) and fibroblast growth factor (FGF) are valuable markers used for cell regeneration. The aim of this study was to investigate the potential effects of PRP treatment on the neomucosa formation, a potential technique for increasing the intestinal surface area in patients with short bowel syndrome (SBS).

Materials and methods

Thirty-two male Wistar-Hannover rats were divided into: sham, control, PRP-treated and last group for PRP preparation (n=8). Plasma levels of VEGF, TGFβ, EGF and FGF were quantified by ELISA. En-bloc resection of anastomotic part was performed and stained with hematoxylin-eosin.

Results

VEGF, FGF, TGFβ and EGF levels were found significantly increased in PRP-treated group compared to others (p<0.001). Neomucosa formation was observed in experimental groups but the area increased significantly in PRP group, compared to other groups (p<0.001).

Conclusion

PRP therapy in gastrointestinal anastomoses is truly beneficial and surgically applicable treatment in SBS patients.

Öz

Amaç

Otolog trombositten zengin plazma (TZP), plazmadaki trombositlerden elde edilen trombosit konsantrasyonudur. İyileşme sürecinde, trombositler aktive olur ve sonra inflamatuar kaskad ve iyileşme sürecini uyaran maddeleri granüllerinden salarlar. Trombosit kökenli büyüme faktörü, vasküler endotelyal büyüme faktörü (VEGF), dönüştürücü büyüme faktörü beta (TGFβ), epidermal büyüme faktörü (EGF) ve fibroblast büyüme faktörü (FGF) hücre yenilenmesinde kullanılan değerli belirteçlerdir. Bu çalışmanın amacı, kısa bağırsak sendromlu hastalarda (KBS) intestinal yüzey alanını arttırmak için kullanılan potansiyel bir teknik olan neomukoza oluşumunda TZP uygulamasının olası etkilerini araştırmaktır.

Gereç ve Yöntem

Otuz iki erkek Wistar-Hannover sıçan, şam, kontrol ve TZP uygulanan ve TZP hazırlanan gruplara ayrıldı (n=8). VEGF, TGFβ, EGF, ve FGF plazma düzeyleri ELISA ile belirlendi. Anastomotik parçaların en-blok rezeksiyonu gerçekleştirildi ve hematoksilen-eozin ile boyandı.

Bulgular

VEGF, TGFβ, EGF ve FGF düzeylerinin TZP-uygulanan grupta diğerlerine göre belirgin bir şekilde arttığı bulundu (p<0.001). Neomukoza oluşumu deneysel gruplarda gözlendi fakat diğer gruplarla karşılaştırıldığında neomukoza oluşum alanı TZP grubunda belirgin şekilde arttı (p<0.001).

Sonuç

KBS’lu hastaların gastrointestinal anastomozlarında TZP tedavisi gerçekten faydalı ve cerrahi olarak uygulanabilir bir tedavidir.

Keywords: Growth factor; Neomucosa; Platelet rich plasma; Rat; Short bowel syndrome

Anahtar Kelimeler: Büyüme faktörü; Neomukoza; Trombositten zengin plazma; Sıçan; Kısa bağırsak sendromu

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About the article

Corresponding author: Dr. Binboğa Sinan, Department of General Surgery, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, Zuhuratbaba District. Tevfik Saglam Street, Bakirkoy, Istanbul, Turkey, Phone: +90 506 498 31 45, Fax: +90 212 414 64 94


Received: 2018-03-16

Accepted: 2018-06-26

Published Online: 2018-09-27


Conflict of interest statement: All authors declared that there are no financial relationships with any organizations that might have an interest in the submitted work; no other relationships or activities that could appear to have influenced the submitted work.


Citation Information: Turkish Journal of Biochemistry, Volume 44, Issue 1, Pages 32–40, ISSN (Online) 1303-829X, DOI: https://doi.org/10.1515/tjb-2018-0098.

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