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Turkish Journal of Biochemistry

Türk Biyokimya Dergisi


IMPACT FACTOR 2018: 0.329

CiteScore 2018: 0.28

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Source Normalized Impact per Paper (SNIP) 2018: 0.169

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1303-829X
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Volume 44, Issue 5

Issues

Plasma homocysteine and aminothiol levels in idiopathic epilepsy patients receiving antiepileptic drugs

Antiepileptik ilaç alan idiyopatik epilepsi hastalarında plazma homosistein ve aminotiyol düzeyleri

Dilber Çoban Ramazan / Ülker Anadol
  • Department of Neurology, Ümraniye State Hospital, School of Medicine, Health Sciences University, Ümraniye, İstanbul, Turkey
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/ A. Destina Yalçın
  • Department of Neurology, Ümraniye State Hospital, School of Medicine, Health Sciences University, Ümraniye, İstanbul, Turkey
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/ A. Süha YalçınORCID iD: https://orcid.org/0000-0002-3527-631X
Published Online: 2019-04-15 | DOI: https://doi.org/10.1515/tjb-2018-0218

Abstract

Objective

Homocysteine is a sulfur containing amino acid that is formed during methionine metabolism. Patients under long-term antiepileptic drug treatment often have hyperhomocysteinemia. These patients have low levels of serum folate, vitamin B12 and vitamin B6, all of which are associated with homocysteine metabolism. We have investigated the effects of valproic acid and new generation antiepileptic drugs (lamotrigine and levetiracetam) on plasma levels of homocysteine and aminothiols as well as serum vitamin B12 and folic acid.

Materials and methods

Forty-seven idiopathic epileptic patients on antiepileptic drugs were compared with 38 age-matched healthy controls. Commercial immunoassay methods were used for vitamin B12 and folic acid analyses. Homocysteine, cysteine, cysteinylglycine and glutathione levels were determined by high performance liquid chromatography.

Results

There was no significant difference in patient and control values in terms of vitamin B12, folic acid and homocysteine. Valproic acid and lamotrigine seemed to effect aminothiol redox status. Glutathione levels of epileptic patients receiving valproic acid and lamotrigine were higher than controls.

Conclusion

Our results suggest that redox homeostasis may be impaired and glutathione synthesis increased in response to the oxidative stress caused by antiepileptic drug use.

Öz

Amaç

Homosistein metiyonin metabolizması sırasında oluşan kükürtlü bir amino asittir. Uzun süre antiepileptik ilaç tedavisi alan hastalarda hiperhomosisteinemi görülebilmektedir. Bu hastalarda homosistein metabolizması ile ilişkili olarak serum folat, B12 vitamini ve B6 vitamini düzeyleri de düşüktür. Bu çalışmada valproik asit ile yeni nesil antiepileptiklerden lamotrijin ve levetirasetamın plazma homosistein ve aminotiyol düzeyleri ile serum B12 vitamini ve folik asit düzeyleri üzerine etkisini araştırdık.

Gereç ve Yöntemler

Antiepileptik ilaç alan 47 idiyopatik epilepsi hastasının değerleri ile aynı yaş grubundaki 38 sağlıklı kontrol değerleri karşılaştırıldı. B12 vitamini ve folik asit ticari immunoassay yöntemleri, homosistein, sistein, sisteinilglisin ve glutatyon ise yüksek performanslı sıvı kromatografisi ile analiz edildi.

Bulgular

B12 vitamini, folik asit ve homosistein açısından hasta ve kontrol değerlerinde bir farklılık görülmedi Valproik asit ve lamotrijinin aminotiyol redoks durumunu etkilediği gözlendi. Bu ilaçları alan hastalarda glutatyon değerleri kontrollerden yüksek bulundu.

Sonuç

Bulgularımız antiepileptik ilaç kullanımının oksidatif strese yanıt olarak redoks dengesinde bozukluğa ve glutatyon sentezinde artışa yol açtığını düşündürmektedir.

Keywords: Antiepileptic drugs; Vitamin B12; Folic acid; Homocysteine; Glutathione; Idiopathic epilepsy patients

Anahtar Kelimeler: Antiepileptik ilaçlar; B12 Vitamini; Folik asit; Homosistein; Glutatyon; Idiyopatik epilepsi hastaları

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About the article

Corresponding author: Prof. Dr. A. Süha Yalçın, Department of Biochemistry, School of Medicine, Marmara University, Başıbüyük, Maltepe, 34854 İstanbul, Turkey


Received: 2018-05-29

Accepted: 2018-10-03

Published Online: 2019-04-15

Published in Print: 2019-10-25


Conflict of interest statement: The authors declare that there is no conflict of interests regarding the publication of this article.


Citation Information: Turkish Journal of Biochemistry, Volume 44, Issue 5, Pages 661–666, ISSN (Online) 1303-829X, DOI: https://doi.org/10.1515/tjb-2018-0218.

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