Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Turkish Journal of Biochemistry

Türk Biyokimya Dergisi


IMPACT FACTOR 2018: 0.329

CiteScore 2018: 0.28

SCImago Journal Rank (SJR) 2018: 0.138
Source Normalized Impact per Paper (SNIP) 2018: 0.169

Online
ISSN
1303-829X
See all formats and pricing
More options …
Volume 44, Issue 5

Issues

Enteroprotective effect of Tsukamurella inchonensis on streptozotocin induced type 1 diabetic rats

Tsukamurella inchonensis’in streptozotocin kaynaklı Tip 1 diyabetik sıçanlar üzerindeki bağırsak koruyucu etkisi

Mehran Mesgari-Abbasi / Solin Ghaderi
  • Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Islamic Republic of Iran
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Monire KhordadmehrORCID iD: https://orcid.org/0000-0002-4472-3847 / Katayoon Nofouzi
  • Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Islamic Republic of Iran
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hossein Tayefi-Nasrabadi
  • Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Islamic Republic of Iran
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Graham McIntyre
  • Center for Infectious Diseases and International Health, Windeyer Institute for Medical Sciences, University College London, London, UK
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2019-05-16 | DOI: https://doi.org/10.1515/tjb-2018-0309

Abstract

Objectives

Enteropathy is one of the most important complications of diabetes mellitus. The present study determined the possible effects of Tsukamurella inchonensis (Ti) on diabetes enteropathy on rat small intestine.

Materials and methods

A total of 40 rats were divided into four groups of 10. Diabetes was induced by streptozotocin. Oral administration of Ti at dose of 105 and 107 CFU/rat was performed in two groups continuously for 14 days. The third and fourth groups received normal saline as the diabetic and negative control groups, respectively. The blood and intestine tissue samples were taken on 21st day post treatment for biochemical and pathological evaluations.

Results

Significant differences were found in serum glucose, cholesterol and triglycerides values together with in CAT and SOD activities, MDA level and IL-6 concentration in both Ti treated groups in comparison with the diabetic rats. Moreover, there were severe pathological changes including degeneration of intestinal mucosa, mononuclear cell infiltration, decreasing number of goblet cells and villous length associated with increasing in villous thickness on the diabetic rats which markedly attenuated in both Ti recipient groups.

Conclusion

In conclusion, it seems that oral administration of Ti may improve intestinal damage in diabetic patients by modulation of intestinal antioxidant defense system.

Öz

Amaç

Enteropati, diabetes mellitusun en önemli komplikasyonlarından biridir. Bu çalışma Tsukamurella inchonensis’in (Ti) sıçan ince bağırsağında diyabet enteropatisi üzerindeki olası etkilerini belirledi.

Gereç ve Yöntem

Toplam 40 sıçan, her birinde 10 sıçan bulunan dört gruba ayrıldı. Diyabet, streptozotosin tarafından uyarıldı. Ti oral yolla 105 ve 107 CFU/sıçan dozunda iki gruba 14 gün boyunca sürekli olarak verildi. Üçüncü ve dördüncü gruplara sırasıyla diyabetik ve negatif kontrol grubu olarak normal salin verildi. Kan ve bağırsak doku örnekleri, biyokimyasal ve patolojik değerlendirmeler için uygulamadan sonraki 21. günde alındı.

Bulgular

Serum glukoz, kolesterol ve trigliserit değerlerinde, CAT ve SOD aktiviteleri, MDA düzeyi ve IL-6 konsantrasyonlarında her iki Ti grubunda da diyabetik sıçanlara kıyasla anlamlı fark bulundu. Ayrıca, diyabetik sıçanlarda intestinal mukoza dejenerasyonu, mononüklear hücre infiltrasyonu, villöz kalınlığının artmasıyla ilişkili villöz uzunluğunun ve goblet hücrelerinin sayısının azalmasını içeren ciddi patolojik değişiklikler vardı. Bu durum her iki Ti alıcı grubunda da belirgin şekilde azalmıştı.

Sonuç

Sonuç olarak, Ti’nin oral uygulamasının, diyabetik hastalarda bağırsak antioksidan savunma sisteminin modülasyonu yoluyla bağırsak hasarını iyileştirebileceği görülmektedir.

Keywords: Tsukamurella inchonensis; Diabetes mellitus; Streptozotocin; Enteropathy; Oxidative stress

Anahtar kelimeler: Tsukamurella inchonensis; Şeker hastalığı; Streptozotosin; enteropati; Oksidatif stres

References

  • 1.

    Alberti KG, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 1998;15:539–53.PubMedCrossrefGoogle Scholar

  • 2.

    Association AD. Diagnosis and classification of diabetes mellitus. Diabetes Care 2010;33:S62–9.PubMedCrossrefGoogle Scholar

  • 3.

    Boutayeb A. The double burden of communicable and non-communicable diseases in developing countries. Trans R Soc Trop Med Hyg 2006;100:191–9.PubMedCrossrefGoogle Scholar

  • 4.

    Bytzer P, Talley NJ, Leemon M, Young LJ, Jones MP, Horowitz M. Prevalence of gastrointestinal symptoms associated with diabetes mellitus: a population-based survey of 15000 adults. Arch Intern Med 2001;161:1989–96.PubMedCrossrefGoogle Scholar

  • 5.

    Phillips LK, Rayner CK, Jones KL, Horowitz M. An update on autonomic neuropathy affecting the gastrointestinal tract. Curr Diab Rep 2006;6:417–23.CrossrefPubMedGoogle Scholar

  • 6.

    Pimentel M. Review of rifaximine as treatment for small intestinal bacterial overgrowth and irritable bowel syndrome. Expert Opin Investig Drugs 2009;18:349–58.PubMedGoogle Scholar

  • 7.

    Krishnan B, Babu S, Walker J, Walker AB, Pappachan JM. Gastrointestinal complications of diabetes mellitus. World J Diabet 2013;4:51–63.CrossrefGoogle Scholar

  • 8.

    Russo A, Botten R, Kong MF, Chapman IM, Fraser RJ, Horowitz M, et al. Effects of acute hyperglycaemia on anorectal motor and sensory function in diabetes mellitus. Diabet Med 2004;21:176–82.CrossrefPubMedGoogle Scholar

  • 9.

    Stanford J, Stanford C. Mycobacteria and their world. Int J Mycobacteriol 2012;1:3–12.PubMedCrossrefGoogle Scholar

  • 10.

    Fontanella GH, Pascutti MF, Daurelio L, Perez AR, Nocito AL, Wojdyla D, et al. Improved outcome of Trypanosoma cruzi infection in rats following treatment in early life with suspensions of heat-killed environmental Actinomycetales. Vaccine 2007;25:3492–500.Web of SciencePubMedCrossrefGoogle Scholar

  • 11.

    Hansrani M, Stanford J, McIntyre G, Bottasso O, Stansby G. Immunotherapy for the prevention of myointimal hyperplasia after experimental balloon injury of the rat carotid artery. Carotid Artery Dis 2010;61:437–42.Google Scholar

  • 12.

    Tarres MC, del Carmen Gayol M, Picena JC, Alet N, Bottasso O, McIntyre G, et al. Beneficial effects of immunotherapy with extracts derived from Actinomycetales on rats with spontaneous obesity and diabetes. Immunotherapy 2012;4:1–11.Web of ScienceGoogle Scholar

  • 13.

    Nofouzi K, Aghapour M, Baradaran B, Hamidian GR, Zare P, Stanford J, et al. Heat-killed Tsukamurella inchonensis reduces lipopolysaccharide-induced inflammatory responses in activated murine peritoneal macrophages. Vet Med 2017;62:668–73.CrossrefWeb of ScienceGoogle Scholar

  • 14.

    Consoli A. Potential side effects to GLP-1 agonists: understanding their safety and tolerability. Expert Opin Drug Saf 2015;14:207–18.Web of SciencePubMedCrossrefGoogle Scholar

  • 15.

    Tietz NW. Clinical guide to laboratory tests, 3rd ed. Philadelphia: W.B. Saunders Company, 1995:509–80.Google Scholar

  • 16.

    Bhor VM, Raghuram N, Sivakami S. Oxidative damage and altered antioxidant enzyme activities in the small intestine of streptozotocin-induced diabetic rats. Int J Biochem Cell Biol 2004;36:89–97.CrossrefPubMedGoogle Scholar

  • 17.

    Marklund S, Marklund G. Involvement of the superoxide anion radical in autoxidation of pyrogallol as a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469–74.PubMedCrossrefGoogle Scholar

  • 18.

    Aebi HE. Catalase. In: Bergmeyer HU, editor. Methods in enzymatic analysis (Vol. III, pp. 278–82). Weinhiem, Germany: Verlag Chemie, 1983:534–48.Google Scholar

  • 19.

    Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animals and tissue by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8.CrossrefPubMedGoogle Scholar

  • 20.

    Ozdemir O, Akalin PP, Baspinar N, Hatipoglu F. Pathological changes in the acute phase of streptozotocin-induces diabetic rats. Bull Vet Inst Pulawy 2009;53:783–90.Google Scholar

  • 21.

    Sakamoto K, Hirose H, Onizuka A, Hayashi M, Futamura N, Kawamura Y, et al. Quantitative study of changes in intestinal morphology and mucus gel on total par-enteral nutrition in rats. J Surg Res 2000;94:99–106.CrossrefGoogle Scholar

  • 22.

    Maisey A. A Practical approach to gastrointestinal complications of diabetes. Diabetes Ther 2016;7:379–86.CrossrefPubMedWeb of ScienceGoogle Scholar

  • 23.

    Bonnefont-Rousselot D, Bastard JP, Jaudon MC, Delattre J. Consequences of the diabetic status on the oxidant/antioxidant balance. Diabetes Metab 2000;26:163–76.PubMedGoogle Scholar

  • 24.

    Halliwell B, Zhao K, Whiteman M. The gastrointestinal tract: a major site of antioxidant action? Free Radic Res 2000;33:819–30.CrossrefGoogle Scholar

  • 25.

    Kakkar R, Kalra J, Mantha SV, Prasad K. Lipid peroxidation and activity of antioxidant enzymes in diabetic rats. Mol Cell Biochem 1995;151:113–9.PubMedCrossrefGoogle Scholar

  • 26.

    Kakkar R, Mantha SV, Radhi J, Prasad K, Kalra J. Antioxidant defense system in diabetic kidney: a time course study. Life Sci 1997;60:667–79.CrossrefGoogle Scholar

  • 27.

    Chen YL, Qiao YC, Pan YH, Xu Y, Huang YC, Wang YH, et al. Correlation between serum interleukin-6 level and type 1 diabetes mellitus: a systematic review and meta-analysis. Cytokine 2017;94:14–20.CrossrefWeb of SciencePubMedGoogle Scholar

  • 28.

    Chang XW, Qin Y, Jin Z, Xi TF, Yang X, Lu XH, et al. Interleukin-6 (IL-6) mediated the increased contraction of distal colon in streptozotocin-induced diabetes in rats via IL-6 receptor pathway. Int J Clin Exp Pathol 2015;8:4514–24.Google Scholar

  • 29.

    Andersen JK. Oxidative stress in neurodegeneration: cause or consequence? Nat Med 2004;10(Suppl):18–25.CrossrefGoogle Scholar

  • 30.

    Banan A, Choudhary S, Zhang Y, Fields JZ, Keshavarzian A. Ethanol-induced barrier dysfunction and its prevention by growth factors in human intestinal monolayers: evidence for oxidative and cytoskeletal mechanisms. J Pharmacol Exp Ther 1999;291:1075–85.PubMedGoogle Scholar

  • 31.

    Shabanzadeh S, Shapoori M, Sheikhzadeh N, Nofouzi K, Khani-Oushani A, Najafi-Enferadi MH, et al. Growth performance, intestinal histology, and biochemical parameters of rainbow trout (Oncorhynchus mykiss) in response to dietary inclusion of heat-killed Gordonia Bronchialis. Fish Physiol Biochem 2016;42:65–71.PubMedCrossrefWeb of ScienceGoogle Scholar

  • 32.

    Caspary WF. Physiology and pathophysiology of intestinal absorption. Am J Clin Nutr 1992;55:299–308.CrossrefGoogle Scholar

  • 33.

    Shamoto K, Yamauchi K. Recovery responses of chick intestinal villus morphology to different refeeding procedures. Poult Sci 2000;79:718–23.PubMedCrossrefGoogle Scholar

  • 34.

    Xu ZR, Hu CH, Xia MS, Zhan XA, Wang MQ. Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poult Sci 2003;82:1030–6.PubMedCrossrefGoogle Scholar

  • 35.

    Tucker LA, Taylor-Pickard JA. Interfacing, immunity, gut health and performance, 1st ed. UK: Nottingham University Press, 2004:736–52.Google Scholar

  • 36.

    Sklan D. Early gut development. In: Tucker LA, Taylor-Pickard JA, editors. The Interaction between feed, gut health and humanity. Interfacing immunity, gut health and performance. UK: Nottingham University Press, 2004:9–32.Google Scholar

About the article

Received: 2018-07-24

Accepted: 2018-11-27

Published Online: 2019-05-16

Published in Print: 2019-10-25


Conflict of interest statement: There is no conflict of interests regarding the publication of this article.


Citation Information: Turkish Journal of Biochemistry, Volume 44, Issue 5, Pages 683–691, ISSN (Online) 1303-829X, DOI: https://doi.org/10.1515/tjb-2018-0309.

Export Citation

©2019 Walter de Gruyter GmbH, Berlin/Boston.Get Permission

Comments (0)

Please log in or register to comment.
Log in