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Translational Neuroscience

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Protective effect of tea polyphenols on the blood-brain barrier

Rongliang Xue
  • Department of Anesthesia, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China
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/ Jianrui Lv
  • Department of Anesthesia, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China
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/ Jing Gao
  • Department of Anesthesia, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China
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/ Rongguo Fu
  • Department of Nephrology, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, P.R. China
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/ Wei Li
  • Department of Anesthesia, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China
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/ Xiaoming Lei
  • Department of Anesthesia, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China
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/ Gang Wu
  • Department of Anesthesia, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China
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/ Li Xue
  • Department of Anesthesia, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China
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/ Zhenni Zhang
  • Department of Anesthesia, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, Shaanxi Province, P.R. China
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Published Online: 2013-09-13 | DOI: https://doi.org/10.2478/s13380-013-0133-2

Abstract

This study was to investigate the protective effects of tea polyphenols on the blood-brain barrier (BBB) of rats with global cerebral ischemia/reperfusion (GCIR) injury. Sprague Dawley rats underwent four-vessel occlusion to construct the model of GCIR. Half an hour before complete occlusion, they were treated with tea polyphenols (TP) (6.4%; 100 or 200 mg/kg) via tail intravenous injection. 24 h after reperfusion, BBB permeability was evaluated by measuring brain water content (BWC) and residual amount of Evan’s blue dye in cerebral tissue. In addition to this, MMP-9 and collagen IV protein expression in cerebral tissue were also detected using immunohistochemistry. ANOVA and SNK-q were used to do statistical analysis. Statistical significance was considered at P<0.05. Compared to the untreated, the TP-treated rats had significantly decreased BWC (P<0.05), decreased residual amount of Evan’s blue dye in cerebral tissue (P<0.05), down-regulated MMP-9 (P<0.05) and up-regulated collagen IV expression in brain tissue (P<0.05). It can be concluded from these findings that TP may reduce the MMP-9 mediated collagen IV degradation caused by GCIR to protect the BBB.

Keywords: Global cerebral ischemia/reperfusion (GCIR); Blood-brain barrier (BBB); Tea polyphenols (TP); Collagen IV; Matrix metallopeptidase 9 (MMP-9)

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About the article

Published Online: 2013-09-13

Published in Print: 2013-09-01


Citation Information: Translational Neuroscience, Volume 4, Issue 3, Pages 295–301, ISSN (Online) 2081-6936, ISSN (Print) 2081-3856, DOI: https://doi.org/10.2478/s13380-013-0133-2.

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© 2013 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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