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Vestnik Zoologii

The Journal of National Academy of Sciences of Ukraine, Schmalhauzen Institute of Zoology

6 Issues per year


SCImago Journal Rank (SJR) 2015: 0.266
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Online
ISSN
2073-2333
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Volume 48, Issue 4 (Aug 2014)

Issues

Modified Method of Metaphase Plates Obtaining for Polyploid Fish Genera Carassius and Cobitis Karyotyping (Actinopterygii, Cypriniformes)

P. P. Pukhtayevych
  • Franko Zhytomyr State University, V. Berdychivs’ka str., 40, Zhytomyr, 10002 Ukraine
  • Email:
Published Online: 2014-08-23 | DOI: https://doi.org/10.2478/vzoo-2014-0045

Abstract

Modern methods for obtaining metaphase plates from the somatic cells of fish, most effective in the study of polyploid species, were selected and tested. The technique with CoCl2 and colchicine treatment is recommended on the basis of empirical data for representatives of the genera Carassius and Cobitis. Th e detailed description of this modified technique and characteristics of metaphase plates of Carassius auratus Linnaeus, 1758, Carassius carassius Linnaeus, 1758 and triploid form of Cobitis taenia Linnaeus, 1758 received under tested method is presented in the paper.

Abstract

Ото- браны и апробированы современные методики получения метафазных пластинок соматических клеток рыб, дающих наибольший эффект при исследовании полиплоидных видов. На основании эмпирических данных в отношении представителей родов Carassius и Cobitis рекомендуется ме- тодика с использованием CoCl2 и колхицина. В статье приведено подробное описание модифи- цированной методики и полученные в результате её применения метафазные пластинки Carassius auratus Linnaeus, 1758, Carassius carassius Linnaeus, 1758 и триплоидной формы Cobitis taenia Linnaeus, 1758.

Keywords: karyology; Osteichthyes; metaphase plate; colchicine

Ключевые слова: кариология; костные рыбы; метафазная пластинка; колхицин.

References

  • Amemiya, C. T., Bickham, J. W., Gold, J. A cell culture technique for chromosome preparation in cyprinid fi shes // Copeia. - 1984. - 1. - P. 232-235.

  • Banerjee, S. K. Iduction of fi sh mitotic division in vivo by concavalin A // Indian J. Exp. Biol. - 1987. - 25, N 8. - P. 556-558.

  • Baruff aldi, A., Lanzara, V., Cucchi, C. Utilization of phytohaemagglutinin for in vivo karyological studies in teleost fi sh // Cytobios. - 1992. - 70. - P. 49-52.

  • Boron, A. Karyotypes of diploid and triploid silver crucian carp Carassius auratus gibelio (Bloch) // Cytobios. -1994. - 80. - P. 117-124.

  • Cucchi, C., Baruff aldi, A. La fenilidrazina come mitotico stimulante // Boll. Soc. Ital. Biol. Sper. - 1988. - 64, N 5. - P. 419-423.

  • Cucchi, C., Baruff aldi, A. A new method for karyological studies in teleost fi shes // J. Fish. Biol. - 1990. - 37, N 1. - P. 71-75. [Crossref]

  • Fames, P., Barker, B. E., Brownhill, L. E., Fanger, H. Mitogenic activity in Phytolacca americana (pokeweed) // Lancet. - 1964. - 2. - P. 1100-1101.

  • Gold, J. R. Cytogenetics. // Fish Physiology / Eds W. S. Hoar, D. J. Randall, J. R. Bret. - New York : Academic Press, 1979. - P. 353-405.

  • Gold, J. R., Li, Y. C., Shipley, N. S., Powers, P. K. Improved methods for working with fi sh chromosomes with a review of metaphase chromosome banding // J. Fish Biology. - 1990. - 37, N 4. - P. 563-575.

  • Hartley, S. E., Horne, M. T. A method for obtaining mitotic fi gures from blood leucocyte cultures of rainbow trout, Salmo gairdneri // J. Fish Biology. - 1983. - 22, is. 1. - P. 77-82.

  • Hartley, S. E., Horne, M. T. Cytogenetic techniques in fi sh genetics // J. Fish Biology. - 1985. - 26. - P. 575-582.

  • Kalous, L., Knytl, M., Krajakova, L. Usage of non-destructive method of chromosome preparation applied on silver Prussian carp (Carassius gibelio) // Proceedings of the Workshop on Animal Biodiversity (7 July, 2010). - Prague, 2010. - P. 57-60.

  • Kligherman, A. D., Bloom, S. E. Rapid chromosome preparation from solid tissues of fi shes // J. Fisheries Research Board of Canada. - 1977. - 34, N 2. - P. 266-269.

  • Lakra, W. S., Bhondae, R. R. Development of primary cell culture from the caudal fi n of an Indian major carp, Labeo rohita (Ham.) // Asian Fisheries Science Hereditas. - 1996. - 6. - P. 149-152.

  • Levan, A., Fredga, K., Sandberg, A. A. Nomenclature for centromeric position on chromosomes // Hereditas. -1964. - 52. - P. 201-220.

  • Lin, Y.-H. A PHA injection method in vivo for the rapid obtainment of large number of metaphase fi gers from kidney cells of teleosts // J. Fish. China. - 1982. - 3. - P. 201-208.

  • MacGregor, H., Varley, J. Working with animal chromosomes. - Moscow : Mir, 1986. - 272 p. - Russian : Макгрегор Г., Варли Д. Методы работы с хромосомами животных.

  • Manilo, V. V. Karyotypes of gecko from the genera Alsophylax and Grossobamon // Bull. Zoology. - 1986. - 20, N 5. - P. 46-54. - Russian : Манило В.В. Кариотипы гекконов родов Alsophylax и Grossobamon.

  • Ojima, Y., Kurishita, A. A new method to increase the number of mitotic cells in the kidney tissue for fi sh chromosome studies // Proc. Jap. Acad. Ser. B. - 1980. - 56. - P. 610-615.

  • Oliveira, C., Almeida-Toledo, L. F., Foresti, F., Toledo-Filno, S. A. Supernumerary chromosomes, robertsonian rearrangement and multiple NORs in Corydoras aeneus (Pisces, Siluriformes, Callichthyidae) // Caryologia. - 1988. - 41. - P. 227-236.

  • Orlov, V. N., Bulatova, N. Sh. Comparative cytogenetics and karyosystematics of mammals. - Moscow : Nauka, 1983. - 405 p.- Russian : Орлов В. Н., Булатова Н. Ш. Сравнительная цитогенетика и карио- систематика млекопитающих.

  • Rab, P., Roth, P. Studenokrevni obratlovci (Cold-blooded vertebrates) // Methods of chromosome analysis / Eds P. Baliček, J. Forejt, J. Rubeš. - Brno : Cytogenet. Sect. Cs. Biol. Soc., 1988. - P. 115-124.

  • Romanovsky, D. L. Zur Frage der Parasitologie und Th erapie der Malaria // St. Petersburg Med. Wochenschr. -1891. - 34. - S. 297-302.

  • Simon, R. C. Chromosome morphology and species evolution in the fi ve North American species of Pacifi c salmon (Oncorhynchus) // J. Morphol. - 1963. - 112. - P. 77-97.

  • Vasiliev, V. P. Evolutionary karyology of fi sh. - Moscow : Nauka, 1985. - 299 p. - Russian : Васильев В. П. Эволюционная кариология рыб.

  • Volker, M, Kullmann, H. Sequential chromosome banding from single acetic acid fi xed embryos of Chromaphyosemion killifi shes (Cyprinodontiformes, Nothobranchiida) // Cybium. - 2006. - 30, N 2. - P. 171-176.

  • Webb, M. Th e biological action of cobalt and other metals. III. Chelation of cations by dihydrolipoic acid // Biochimica et Biophysica Acta. - 1962. - 65. - P. 47-65.

About the article

Received: 2013-11-20

Accepted: 2014-04-08

Published Online: 2014-08-23

Published in Print: 2014-08-01


Citation Information: Vestnik Zoologii, ISSN (Online) 2073-2333, DOI: https://doi.org/10.2478/vzoo-2014-0045. Export Citation

© by P. P. Pukhtayevych. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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