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Vestnik Zoologii

The Journal of National Academy of Sciences of Ukraine, Schmalhauzen Institute of Zoology

6 Issues per year

Cite Score 2016: 0.35

SCImago Journal Rank (SJR) 2016: 0.300
Source Normalized Impact per Paper (SNIP) 2016: 0.496

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Volume 48, Issue 4


Modified Method of Metaphase Plates Obtaining for Polyploid Fish Genera Carassius and Cobitis Karyotyping (Actinopterygii, Cypriniformes)

P. P. Pukhtayevych
Published Online: 2014-08-23 | DOI: https://doi.org/10.2478/vzoo-2014-0045


Modern methods for obtaining metaphase plates from the somatic cells of fish, most effective in the study of polyploid species, were selected and tested. The technique with CoCl2 and colchicine treatment is recommended on the basis of empirical data for representatives of the genera Carassius and Cobitis. Th e detailed description of this modified technique and characteristics of metaphase plates of Carassius auratus Linnaeus, 1758, Carassius carassius Linnaeus, 1758 and triploid form of Cobitis taenia Linnaeus, 1758 received under tested method is presented in the paper.


Ото- браны и апробированы современные методики получения метафазных пластинок соматических клеток рыб, дающих наибольший эффект при исследовании полиплоидных видов. На основании эмпирических данных в отношении представителей родов Carassius и Cobitis рекомендуется ме- тодика с использованием CoCl2 и колхицина. В статье приведено подробное описание модифи- цированной методики и полученные в результате её применения метафазные пластинки Carassius auratus Linnaeus, 1758, Carassius carassius Linnaeus, 1758 и триплоидной формы Cobitis taenia Linnaeus, 1758.

Keywords: karyology; Osteichthyes; metaphase plate; colchicine

Ключевые слова: кариология; костные рыбы; метафазная пластинка; колхицин.


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About the article

Received: 2013-11-20

Accepted: 2014-04-08

Published Online: 2014-08-23

Published in Print: 2014-08-01

Citation Information: Vestnik Zoologii, Volume 48, Issue 4, Pages 371–376, ISSN (Online) 2073-2333, DOI: https://doi.org/10.2478/vzoo-2014-0045.

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© by P. P. Pukhtayevych. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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